A5101834670- dental development and anomalies
- Dental Trauma and Treatments
- Salivary Gland Disorders and Functions
- Oral and Maxillofacial Pathology
- MicroRNA in disease regulation
- Periodontal Regeneration and Treatments
- Bacteriophages and microbial interactions
- Lipid metabolism and biosynthesis
- Fatty Acid Research and Health
- Cancer-related molecular mechanisms research
- Transgenic Plants and Applications
- Mesenchymal stem cell research
- Bone Metabolism and Diseases
- Cancer, Hypoxia, and Metabolism
- Crystal structures of chemical compounds
- Autophagy in Disease and Therapy
- Nitric Oxide and Endothelin Effects
- Amino Acid Enzymes and Metabolism
- Antimicrobial Peptides and Activities
- Cleft Lip and Palate Research
- Cancer-related gene regulation
- Neurological Disease Mechanisms and Treatments
- Phosphodiesterase function and regulation
- RNA modifications and cancer
- Wnt/β-catenin signaling in development and cancer
Stomatology Hospital
2022-2024
Zhejiang University
2022-2024
Capital Medical University
2015-2023
Beijing Obstetrics and Gynecology Hospital
2023
The Third Affiliated Hospital of Guangzhou University of Traditional Chinese Medicine
2018-2022
Guangzhou University of Chinese Medicine
2017-2022
Weatherford College
2021
Cells Therapy (Poland)
2021
Nanjing General Hospital of Nanjing Military Command
2016
Nanjing University
2016
microRNAs (miRNAs) act as regulatory signals for maintaining stemness, self-renewal, and differentiation of mesenchymal stem cells (MSCs), but whether miRNAs modulate the immunoregulatory function MSCs remains largely unknown. Here, we show that miR-21 negatively regulates activity cytokine transforming growth factor-β1 (TGF-β1) in MSCs. Consistently, bone marrow (BMMSCs) from miR-21(-/-) mice enhanced immunosuppressive by more TGF-β1 secretion induce CD4(+) Foxp3(+) T compared with...
Management of salivary gland hypofunction caused by irradiation (IR) therapy for head and neck cancer remains lack effective treatments. Salivary glands, especially the parotid gland, actively uptake dietary nitrate secrete it into saliva. Here, we investigated effect on prevention treatment IR-induced in miniature pigs, elucidated underlying mechanism human cells. We found that administration prevented damage a dose-dependent manner, maintaining function irradiated tissue. Nitrate could...
Inorganic nitrate is an indispensable nutrient that has been used in experimental studies for the prevention and treatment of several diseases. However, short half-life limits its clinical application. To increase usability overcome challenges traditional combination drug discovery through large-scale high-throughput biological experiments, we developed a swarm learning-based prediction system identified vitamin C as choice to be combined with nitrate. Employing microencapsulation...
Abstract Objectives Whole‐tooth regeneration for tooth loss has long been a goal of dentistry. There is also an increasing demand to carry out pre‐clinical in vitro and vivo research methods large animal model similar human. The miniature pig proven be alternative as mammal owing its many similarities However, whole‐tooth remains challenge. Here, we investigated the feasibility cell re‐association‐based pigs. Materials Single cells from forth deciduous molar germs (p4) were reconstituted...
Although mouse models of epithelial–mesenchymal interaction-based whole-tooth regeneration have been generated, whether this strategy can be applied to humans remains unknown and preclinical investigations using a large animal model are required. In study, we describe the development feasible methods for in swine model. We characterized odontogenesis dissociated single cells from early-stage tooth germs miniature pigs (minipigs) vitro by tracking morphogenesis reassociated cell pellets an...
Bushen Huoxue decoction (BHD) has a significant effect on fracture rehabilitation, yet its underlying mechanism is still unknown. The purpose of this study was to explore whether BHD promotes bone marrow mesenchymal stem cell (BMSC) migration through the Wnt5a signalling pathway. extracted by petroleum, and composition analysed. Cell viability in presence various concentrations for 24, 48 72 h measured using Counting Kit-8 assay. Transwell assays wound healing were used observe ability...
Bone marrow mesenchymal stem cell (BMSC) transplants are promising for the treatment of certain central nervous system diseases. However, oxidative stress is one major factors that may limit survival transplanted BMSCs. The present study investigated effect pretreatment with gigantol on hydrogen peroxide (H2O2)‑induced apoptosis in rat BMSCs (rBMSCs) and potential underlying mechanisms. results demonstrated significantly inhibited H2O2‑induced rBMSCs. rBMSCs were incubated 600 µM H2O2...
Abnormalities of tooth number in humans, such as agenesis and supernumerary formation, are closely related to diphyodont development. There is an increasing demand understand the molecular cellular mechanisms behind development through use large animal models, since they most similar mechanism human However, attempting study remains challenging due size, prolonged growth stage, embryo manipulation. Here, we characterized expression possible genes for odontogenesis organoid bud from single...
Dens invaginatus (DI) is a developmental anomaly, Oehlers Type III DI the most complex type and early diagnosis treatment complicated challenging. This report presents rare case of IIIb associated with periapical lesion in bilateral immature permanent mandibular central incisors.An eight-year-old boy referred to our clinic manifesting pain along swelling incisors for past one month. Radiographic examination showed radiolucency exhibiting scantly defined border, as well an invagination which...
Bone fractures are very common, and above 5% of the impaired, leading to nonunions severe disablilities.The traditional Chinese medicine Bushen Huoxue decoction (BHD) has been used treat fracture in China.Our previous report found that BHD promotes migration rat mesenchymal stem cells (rMSCs) by activating Wnt5a signaling pathway.However, whether how miRNAs involved modulating rMSCs induced not explored.In present study, miRNA microarray analysis further validation real-time quantitative...
Phosphodiesterase 4 (PDE4) has four subtypes: PDE4A, PDE4B, PDE4C and PDE4D. The expression of PDE4 subtypes in microglial cells the specific contribution each subtype to inflammation remain unclear. In this study, primary was assayed. Primary were then transfected with small interfering RNA (siRNA) against subtype. A-D knockdown confirmed by quantitative polymerase chain reaction. Secreted cytokines supernatant intracellular cyclic adenosine monophosphate (cAMP) levels measured. effect...
Miniature pigs, a valuable alternative model for understanding human tooth development, have deciduous teeth from all four families that are replaced once by permanent molars. The extracellular matrix (ECM) supports cells and maintains the integrity of germs during development. However, details on role ECM in development poorly understood. Here, we performed long noncoding RNA (lncRNA) messenger (mRNA) expression profiles components sequencing miniature pigs. From early cap to late bell...
Background Hip fractures are a common type of fracture in the elderly and characterized by many complications high mortality. Many topics concerning postoperative rehabilitation hip people still remain controversial, global research trend this field has not yet been well studied. The aim present study was to illustrate overall knowledge structure, development trends, hot spots people. Methods Articles reviews regarding from 2001 2021 were identified Web Science database. An online...
Infraocclusion refers to a tooth located below the occlusal surface compared adjacent teeth long after it should have reached occlusion. Ankylosis is frequently associated with infraocclusion of primary molars, but challenging distinguish between two conditions. Surgical luxation and orthodontic traction been applied for treatment ankylosed, infraoccluded permanent teeth, reports on molars are scarce.