A5038790107- Birth, Development, and Health
- Pregnancy and preeclampsia studies
- Neuroinflammation and Neurodegeneration Mechanisms
- Adipose Tissue and Metabolism
- Multiple Sclerosis Research Studies
- Lipid metabolism and biosynthesis
- Assisted Reproductive Technology and Twin Pregnancy
- Reproductive Biology and Fertility
- Barrier Structure and Function Studies
- Ovarian function and disorders
- Neurogenesis and neuroplasticity mechanisms
- Diet and metabolism studies
- Cardiovascular and Diving-Related Complications
- Diet, Metabolism, and Disease
- Cerebrospinal fluid and hydrocephalus
- Mitochondrial Function and Pathology
- Adipokines, Inflammation, and Metabolic Diseases
- Neuroscience of respiration and sleep
- High Altitude and Hypoxia
- Gestational Diabetes Research and Management
- Immunotherapy and Immune Responses
- Metabolism, Diabetes, and Cancer
- Chronic Obstructive Pulmonary Disease (COPD) Research
University of Bern
2016-2023
University Hospital of Bern
2013-2017
University Hospital of Basel
2010
Children conceived by assisted reproductive technologies (ART) display a level of vascular dysfunction similar to that seen in children mothers with preeclamspia. The long-term consequences ART-associated disorders are unknown and difficult investigate healthy children. Here, we found vasculature from mice generated ART endothelial increased stiffness, which translated into arterial hypertension vivo. Progeny male also exhibited dysfunction, suggesting underlying epigenetic modifications....
Abstract Meninges cover the surface of brain and spinal cord contribute to protection immune surveillance central nervous system (CNS). How meningeal layers establish CNS compartments with different accessibility cells mediators is, however, not well understood. Here, using 2-photon imaging in female transgenic reporter mice, we describe VE-cadherin at intercellular junctions arachnoid pia mater that form leptomeninges border subarachnoid space (SAS) filled cerebrospinal fluid (CSF)....
Abstract Signaling by insulin-like growth factor-1 (IGF-1) is essential for the development of central nervous system (CNS) and regulates neuronal survival myelination in adult CNS. In neuroinflammatory conditions including multiple sclerosis (MS) its animal model experimental autoimmune encephalomyelitis (EAE), IGF-1 can regulate cellular activation a context-dependent cell-specific manner. Notwithstanding importance, functional outcome signaling microglia/macrophages, which maintain CNS...
Abstract Blood-brain barrier (BBB) breakdown and immune cell infiltration into the central nervous system (CNS) are early hallmarks of multiple sclerosis (MS). High numbers CD8 + T cells found in MS lesions, antigen (Ag) presentation at BBB has been proposed to promote entry CNS. Here, we show that brain endothelial process cross-present Ag, leading effector differentiation. Under physiological flow vitro, Ag prevented crawling diapedesis resulting apoptosis breakdown. Brain vivo was limited...
Abstract Oligodendrocytes (ODCs) are myelinating cells of the central nervous system (CNS) supporting neuronal survival. Oxidants and mitochondrial dysfunction have been suggested as main causes ODC damage during neuroinflammation observed in multiple sclerosis (MS). Nonetheless, dynamics this process remain unclear, thus hindering design neuroprotective therapeutic strategies. To decipher spatio‐temporal pattern oxidative mitochondria vivo, we created a novel mouse model which ODCs...
High fat diet-induced endotoxaemia triggers low-grade inflammation and lipid release from adipose tissue. This study aims to unravel the cellular mechanisms leading lipopolysaccharide (LPS) effects in human adipocytes. Subcutaneous pre-adipocytes surgically isolated patients were differentiated into mature adipocytes vitro. Lipolysis was assessed by measurement of glycerol mRNA expression pro-inflammatory cytokines evaluated real-time PCR. Treatment with LPS for 24 h induced a dose-dependent...
Assisted reproductive technology (ART) alters glucose homeostasis in mice and humans, but the underlying mechanisms are incompletely understood. ART induces endothelial dysfunction arterial hypertension by epigenetic alteration of nitric oxide synthase (eNOS) gene. In eNOS-deficient mice, insulin resistance is related to impaired stimulation muscle blood flow substrate delivery defective intrinsic skeletal uptake. We therefore assessed tolerance, sensitivity (euglycemic clamp), vivo, uptake...
We previously demonstrated that the sodium/hydrogen exchanger NHA2, also known as NHEDC2 or SLC9B2, is critical for insulin secretion by β–cells. To gain more insights into role of NHA2 on systemic glucose homeostasis, we studied impact loss during physiological aging process and in setting diet-induced obesity. While tolerance was normal at 2 months age, KO mice displayed a significant intolerance 5 12 respectively. An obesogenic high fat diet further exacerbated mice. Insulin levels...
Objective: To study the effects of metformin on lipolysis and hormone sensitive lipase (HSL) phosphorylation in human adipocytes treated with lipolytic inflammatory agents.Methods: Lipolysis status HSL were assessed subcutaneous pre-adipocytes surgically isolated from patients differentiated into mature vitro.Results: Stimulation for 1 h forskolin, isoproterenol IBMX or stimulation 24 LPS, IL-1β TNF-α increased (p < 0.05 vs. basal). The at Ser-554 was decreased while Ser-552 increased....
Assisted reproductive technologies (ART) induce vascular dysfunction and premature ageing in young apparently healthy children mice (Scherrer et al. Circulation 2012; Rexhaj JCI 2013). In adult ART mice, translates into arterial hypertension. Given the age of human population, evolution long‐term consequences induced alterations are unknown. We speculated that persist adolescents hypertension represents first clinically relevant end‐point this problem humans. We, therefore, 5 years after...
Re‐entry high altitude pulmonary edema (HAPE) is a live‐threatening problem that occurs on return from low in otherwise healthy high‐altitude dwellers. The underlying mechanism poorly understood. Circumstantial data suggest vascular dysfunction facilitating hypoxic vasoconstriction plays role. There increasing evidence at dwellers, often associated with alterations of the systemic circulation related to altered redox regulation. We speculated re‐entry HAPE prone subjects display sustained...
In humans, offspring of mothers with preeclampsia display generalized vascular dysfunction and premature cardiovascular morbidity, but underpinning mechanisms possibilities for prevention are unknown. mice, restrictive diet pregnancy (RDP), a model mimicking in insulin resistance offering an opportunity to study interventions aimed at preventing these problems. Sildenafil administration preeclamptic rats increases birth weight the offspring. We assessed function vitro (mesenteric artery...
There is abundant evidence indicating that pathological events during early life predispose to cardiovascular and metabolic diseases later in life. Recent suggests assisted reproductive technologies represent a novel important example of this problem.
In mice, restrictive diet pregnancy (RDP), a model mimicking preeclampsia in humans, induces vascular dysfunction the offspring that is related to decreased nitric oxide (NO) bioavailability. There abundant evidence NO plays an important role regulation of insulin sensitivity animals and humans. We speculated RDP mice are insulin‐resistant. We, therefore, assessed (euglycemic‐hyperinsulinemic clamp studies) control fed with high‐fat (HFD) for 8 wks. The main new finding was HFD induced much...