ABSTRACT Several actinomycetes isolated from nature were able to use both natural rubber (NR) and synthetic cis -1,4-polyisoprene (IR) as a sole source of carbon. According their degradation behavior, they divided into two groups. Representatives the first group grew only in direct contact substrate led considerable disintegration material during cultivation. The second consisted weaker decomposers that did not grow adhesively, indicated by formation clear zones (translucent halos) around...

A cis-1,4-polyisoprene-degrading bacterium (strain Kb2T) was isolated from foul water taken the inside of a deteriorated automobile tyre found on farmer's field in Westfalia, Germany. The strain aerobic, Gram-positive, exhibited orange smooth and rough colonies complex nutrient agar, produced elementary branching hyphae that fragmented into rod/coccus-like elements showed chemotaxonomic markers which were consistent with its classification within genus Gordonia, i.e. presence...

During the last few decades there is growing interest for use of nanoparticles in water and wastewater treatment technology, due to their distinctive physiochemical characteristics.This review outlines conceivable opportunities regarding interaction with biological systems during processes.An overview on recent advances application biofouling prevention disinfection provided.Recent nanoscience allow development nanosensors biomonitoring diagnostic purposes.Due remarkable nanoparticles,...

Twenty-six different bacterial strains were isolated from samples taken locations Dammam, Saudi Arabia, for screening of their polyhydroxyalkanoate (PHA) production capability. The initial was conducted by staining with Sudan Black B and Nile Red, followed examination under fluorescence electron microscopes to characterize PHA granule formation. PHA-producing isolates identified using 16S rRNA gene analyses; the most potent strain as Pseudomonas sp. strain-P(16). capability this in presence...

Background. Lack of knowledge about appropriate handwashing practices has caused great concerns for human health, especially in the risk many communicable diseases. The objective current study is to determine level knowledge, attitudes, and among school students Eastern Province Schools, Saudi Arabia. A cross-sectional survey was recruited from November 2019 March 2020 assess students’ knowledge. reliable questionnaire prepared (Cronbach’s alpha = 0.608) conducted using a two-stage sampling...

Background and Objectives: Despite extensive research on the sources transmission pathways of Hospital-Acquired Infections (HAIs), role cleaning staff as potential vectors has been under-explored. This study addresses gap by examining staff’s in nosocomial infection transmission, focusing pathogenic bacteria fungi colonization. Identifying pathogens harbored that carry risk causing HAIs, especially methicillin-resistant Staphylococcus aureus (MRSA) Enterobacteriaceae. Materials Methods:...

The effect of pretreatment several cis-1,4-polyisoprene containing rubbers on their biodegradability was examined. Tests were carried out with six recently isolated and characterized rubber degrading bacteria belonging to the genera Gordonia (strains Kb2, Kd2 VH2), Mycobacterium, Micromonospora Pseudomonas. All strains able use natural (NR) as well NR latex gloves sole carbon source. Extraction by organic solvents resulted in an enhancement growth for three selected strains. On other hand,...

ABSTRACT Among several bacterial species belonging to the general Gordonia , Mycobacterium Micromonospora Pseudomonas and Rhodococcus only two mycobacterial isolates, fortuitum strain NF4 new isolate ratisbonense SD4, which was isolated from a sewage treatment plant, were capable of utilizing multiply branched hydrocarbon squalane (2,6,10,15,19,23-hexamethyltetracosane) its analogous unsaturated squalene as sole carbon source for growth. Detailed degradation studies high-pressure liquid...

Two cis-1,4-polyisoprene (isoprene rubber) degrading bacteria, strains VH2 and Y2K, were identified as of the species Gordonia polyisoprenivorans belonging to Corynebacterineae, a suborder order Actinomycetales. Both showed characteristic growth degradation isoprene rubber described previously for type strain G. Kd2 (DSM 44302T). For chemotaxonomic properties investigated, DNA–DNA hybridization experiments with revealed affiliation polyisoprenivorans. The comparison 16S rDNA sequences,...

Silver Phosphate, Ag3PO4, being a highly capable clinical molecule, an ultrasonic method was employed to synthesize the M-Ag3PO4, (M = Se, Ag, Ta) nanoparticles which were evaluated for antibacterial and cytotoxicity activities post-characterization. Escherichia coli Staphylococcus aureus used testing effects of sonication on bacterial growth with sub-MIC values M-Ag3PO4 examined. The effect human colorectal carcinoma cells (HCT-116) cervical (HeLa cells) examined by MTT...

High levels of potentially toxic metals and microbes in the atmosphere, especially indoor air, may severely threaten human health. Therefore, concentration associated health risks heavy (Cd, Cr, Pb, Cu, Fe, Mn, Zn), biological pollutants, their risk to were assessed using air condition (AC) filter dust samples. Samples collected from five locations representing agricultural, industrial, residential settings Eastern Province, Kingdom Saudi Arabia. The trace varied considerably among sampling...

ABSTRACT Gordonia westfalica Kb1 and polyisoprenivorans VH2 induce the formation of an extracellular superoxide dismutase (SOD) during poly( cis -1,4-isoprene) degradation. To investigate function this enzyme in G. VH2, sodA gene was disrupted. The mutants exhibited reduced growth liquid mineral salt media containing as sole carbon energy source, no SOD activity detectable supernatants cultures. Growth experiments revealed that SodA is required for optimal on -1,4-isoprene), whereas has...

Bacillus megaterium SW1-2 producing polyhydroxalkanoate (PHA), previously isolated from activated sewage sludge was selected for the study of PHB biopolymer production. Preliminary experiment showed that strain capable 36% cell dry weight (CDW) polymer during growth on basal E2 medium supplemented with glucose, followed by 28, 15 and 8% CDW Na-acetate, lactose or Na-succinate, respectively. Spectroscopic analysis FT-IR revealed characteristic peaks at 1722.8 1276 cm-1 corresponding to C=O...

A drastic level of resource degradation was revealed through a preliminary evaluation (completed in 2016) water quality recreational lake the second industrial city Dammam, Saudi Arabia. The primary signs were foul smell, algal bloom, high turbidity, and lack aquatic life. This study aims to evaluate influence reverse osmosis (RO) on quality. consists two connected lakes (Lakes 1 2), which receive treated effluent from an wastewater treatment plant. Composite samples collected analyze their...

Background: Air pollution, both indoor and outdoor, is often considered as one of the major causes environmental health problems. Usually, air in slaughterhouses heavily contaminated by large quantities dust particles biological nonbiological origin, toxic gases, odors. However, quality was rarely investigated. Objective: To evaluate slaughterhouse environment parameters such particulate, bioaerosol levels. Materials Methods: Indoor outdoor CO, O3, CO2, VOCs, PM10, PM2.5, SO2, NO2 were...