A5007732408- T-cell and B-cell Immunology
- Immunodeficiency and Autoimmune Disorders
- Immune Cell Function and Interaction
- Rheumatoid Arthritis Research and Therapies
- Monoclonal and Polyclonal Antibodies Research
- Systemic Lupus Erythematosus Research
- Immunotherapy and Immune Responses
- Glycosylation and Glycoproteins Research
- Amyloidosis: Diagnosis, Treatment, Outcomes
- Head and Neck Cancer Studies
- Hepatitis C virus research
- Immune Response and Inflammation
- Lymphoma Diagnosis and Treatment
- Cytomegalovirus and herpesvirus research
- Chronic Lymphocytic Leukemia Research
- T-cell and Retrovirus Studies
- Head and Neck Surgical Oncology
- Pancreatitis Pathology and Treatment
- Diabetes and associated disorders
- Cell Adhesion Molecules Research
- Viral-associated cancers and disorders
- Adipokines, Inflammation, and Metabolic Diseases
- CAR-T cell therapy research
- Cytokine Signaling Pathways and Interactions
- Pediatric health and respiratory diseases
Higashi Osaka City General Hospital
2013-2017
Sanofi (United States)
2017
Center for Rheumatology
2014
Osaka Prefecture University
2007
Tuen Mun Hospital
1998-2005
Osaka Minami Medical Center
1995-2002
Osaka University
1985-1995
Ehime University
1989-1993
Saba University School of Medicine
1992
Osaka University Hospital
1987-1989
The aim of this analysis was to evaluate the outcomes patients with nasopharyngeal carcinoma (NPC) treated primarily by external beam irradiation (ERT) and explore for possible ways improve treatment results.One thousand seventy nonmetastatic NPC from 1990 1998 were retrospectively analyzed. distribution according Union Internationale Contre le Cancer (UICC) (1997 edition) staging system at initial diagnosis as follows: stage I, n = 113; IIA, 38; IIB, 360; III, 306; IVA, 136; IVB, 117; T1,...
B-lymphocyte function of 15 patients with primary common varied immunodeficiency or related disease were examined. All had low serum levels IgM, IgG, and IgA, but 12 nearly normal numbers peripheral blood B lymphocytes. Mononuclear cells from assayed for B-cell mitogenic responses to anti-Ig mu chain antibodies Staphylococcus aureus strain Cowan I (referred as I), differentiation Ig-secreting IgA classes in the presence pokeweed mitogen T-cell factor. The all showed profound defects one more...
Persistent diabetes mellitus with marked hyperglycemia was induced in mice by the administration of streptozotocin. In these streptozotocin-induced diabetic mice, resistance to tubercle bacillus challenge and primary as well secondardy humoral immune responses against foreign erythrocytes were markedly depressed. The T-cell function delayed hypersensitivity 2,4-dinitro-1-fluorobenzene bacterial phagocytic activity or peritoneal macrophages contrast, B-cell antibody production T-independent...
ABSTRACT Objectives: We previously found that discrepancy between serum amyloid A (SAA) and C-reactive protein (CRP) levels linked to the difference of SAA/CRP ratio in patients with early rheumatoid arthritis, varied among different patients. This study aimed determine why differed Methods: The (n=466) most inflammatory diseases were enrolled. After examined CRP SAA concentrations, we compared ratio, concentrations men women. Results: dramatically (33.6–0.140). 10 highest ratios all women,...
Stimulation of human B lymphoblastoid cell lines, CESS and SKW6-CL4 with BCDF induced an increase in IgG- IgM-secreting cells, as well a slight IgG IgM expression on their respective surfaces. Biosynthetic labeling demonstrated that stimulation synthesis secretory gamma- mu-chains, precursors. A but significant membrane-bound mu-chains precursors was also observed by stimulation. However, heavy chains much higher than both lines. Pulse-chase experiments showed increased not due to decrease...
Synergistic effects of B cell mitogen Staphylococcus bacteria strain Cowan I (Cowan I) plus T mitogens in generating immunoglobulin-secreting cells (ISC) from human peripheral blood mononuclear (MNC) were investigated. ISC assayed by reverse plaque-forming with protein A-coated red cells. Low concentrations pokeweed (PWM) gave superadditive on induction, 3 to 10 times as many optimal amounts either alone. The together and separately showed similar kinetics ISC; synergy was observed at every...
Abstract In vitro stimulation of the B leukemic cells (B-CLL cells) with normal allogeneic T plus PWM induced IgM secretion and a switching from to IgG production. Induction production in B-CLL was demonstrated by presence same idiotype Ig as that present monoclonal protein patient's serum. Both were required for induction cells, x-irradiated showed comparable helper effect. not only but proliferation cells. Cell division essential differentiation Ig-producing PWM-induced,...
Abstract A number of human B lymphoblast cell lines were tested to find a suitable model for induction immunoglobulin‐secreting cells (ISC) by T replacing factor (TRF). Partially purified TRF was size fractionated from the conditioned medium irradiated (1000 rds) spleen stimulated with pokeweed mitogen. IgM line SKW 6 showed high levels TRF‐stimulated immunoglobulin secretion and chosen cloning experiments. Clone 11 had very low level ISC or without (less than 0.1% ISC). 4 background numbers...
Abstract Statistical analysis of serum immunoglobulin levels in 54 systemic lupus erythematosus (SLE) patients showed a decrease IgM (normal 77 ± 26 mg/dl; P < 0.001) that was closely related to disease duration. The longer the duration SLE, more striking (r = −0.74, P<0.01). Selective deficiency (<47 mg/dl) occurred SLE whose clinically inactive or less active.
The ligand for CD40 (CD40L) is a membrane protein on activated T cells that induces B cell proliferation and differentiation. Several mutations of the CD40L gene were reported responsible defective class switching in an X-linked immunodeficiency with hyper IgM (X-HIM). We studied four affected males from three families found independent including new gene. In every X-HIM patient tested, however, anti-CD40 plus IL-10 did not induce to IgG or IgA, even presence Staphylococcus aureus Cowan I...
Expression of p70/75 IL-2-binding molecules and their functional roles in induction Ig secretion by IL-2 were examined human B cells. IL-2, at high concentrations induced higher levels Staphylococcus aureus strain Cowan I (SAC)-activated cells than low concentrations. About 50% SAC-activated cells, lacking Tac antigen, also responsive to although the required dose was that for Tac-positive H-31 antibody which recognizes antigen did not inhibit both Tac-negative suggesting might induce...
In this report, we examined whether novel interleukin 2 (IL-2) binding molecules (p70/75) are responsible for signal transduction and internalization of IL-2 in T cells by using a monoclonal antibody H-31 to Tac antigens. We found that inhibited the antigens but not molecules. Scatchard plot analysis revealed presence H-31, intermediate affinity sites (Kd = 1 1.5 nM) were detectable number them was similar high receptor (IL-2R) 10 15 pM) absence H-31. Furthermore, kinetics endocytosis via...
Abstract In the course of an analysis lymphocyte functions common variable immunodeficiency patients, we found one patient whose T lymphocytes released B cell differentiation factor (BCDF) and growth (BCGF) without IL 2 production upon stimulation with Con A. The was a 68-yr-old woman hypo-gamma-globulinemia (IgM: 31 mg/dl, IgG: 223 IgA: 23 mg/dl); she suffered from cryptococcal meningitis pulmonary tuberculosis negative result skin tests to PPD antigen. number cells ratio subsets (Leu-2a...
Abstract Using radiolabeled interleukin‐2 (IL‐2), affinity cross‐linking and binding assays revealed that the expression of intermediate‐affinity IL‐2 molecules (p70/75) on freshly prepared T cells from patients with systemic lupus erythematosus (SLE) was significantly decreased in comparison normal subjects. The proliferative response to high doses also reduced SLE. p70/75 reflects hyporespon‐siveness