A5058421297- Fermentation and Sensory Analysis
- Plant Gene Expression Analysis
- Lignin and Wood Chemistry
- Enzyme-mediated dye degradation
- Polysaccharides and Plant Cell Walls
- Cancer, Hypoxia, and Metabolism
- Mitochondrial Function and Pathology
- Polysaccharides Composition and Applications
- Plant biochemistry and biosynthesis
- Plant Pathogens and Fungal Diseases
- Biochemical and biochemical processes
- Advanced biosensing and bioanalysis techniques
- Protein Degradation and Inhibitors
- Glioma Diagnosis and Treatment
- Peptidase Inhibition and Analysis
- Horticultural and Viticultural Research
- Plant Reproductive Biology
Kyoto University
2021-2024
The University of Tokyo
2024
Nagoya University
2013-2019
Single-molecule enzyme activity-based profiling (SEAP) is a methodology to globally analyze protein functions in living samples at the single-molecule level. It has been previously applied detect functional alterations phosphatases and glycosidases. Here, we expand potential for biomarker discovery by developing semi-automated synthesis platform fluorogenic probes that can various peptidases protease activities The peptidase/protease were prepared on basis of 7-amino-4-methylcoumarin...
The woody stems of coniferous gymnosperms produce specialised compression wood to adjust the stem growth orientation in response gravitropic stimulation. During this process, tracheids develop a compression-wood-specific S2 L cell wall layer with lignins highly enriched p-hydroxyphenyl (H)-type units derived from H-type monolignol, whereas produced walls normal are exclusively composed guaiacyl (G)-type G-type monolignol trace amount units. We show that laccases, class lignin polymerisation...
We developed a single-molecule enzyme activity assay platform for NAD(P)+-dependent oxidoreductases, leveraging new NAD(P)H-responsive fluorogenic probe optimized microdevice-based fluorometric detection. This enabled the detection of activities in blood and cerebrospinal fluid (CSF), including lactate dehydrogenase, glucose-6-phosphate hexokinases. demonstrate its potential activity-based diagnosis by detecting altered populations species CSF from liver damage brain tumor patients.
Special xylem tissue called “compression wood” is formed on the lower side of inclined stems when gymnosperms grow a slope. We investigated molecular mechanism compression wood formation. Transcriptome analysis by next-generation sequencing (NGS) was applied to Chamaecyparis obtusa develop catalog general gene expression in differentiating during and normal The output generated 234,924,605 reads 40,602 contigs (mean size = 529 bp). Based sequence similarity search with known proteins, 54.2%...
The secondary cell wall of compression wood tracheids has a highly lignified region (S2 L) in its outermost portion. To better understand the mechanism S2 L formation, we focussed on activity laccase (a monolignol oxidase) and performed situ studies this enzyme differentiating wood. Staining demonstrated that began all layers before onset lignification. We detected no peroxidase (another any layer. Thus, likely plays major role oxidisation during differentiation. Laccase was higher than...
Woody plants develop a specialized secondary xylem known as reaction wood to enable formation of an ideal shape. Reaction in coniferous species is compression wood, and that woody angiosperms tension wood. However, the genus Buxus which classified angiosperm, forms compression-wood-like We investigated mechanism lignification wood: 1) Several lignin synthesis genes were upregulated differentiating microphylla; 2) B. microphylla possesses specific laccase gene expressed specifically (BmLac4);...
SUMMARY The distributions of monolignol glucosides (MLGs) in compression and opposite woods Pinus thunbergii were assessed using cryo‐time‐of‐flight secondary ion mass spectrometry to investigate their involvement lignification. p ‐Glucocoumaryl alcohol (PG) was identified the region differentiating xylem adjacent cambial zone only wood, whereas coniferin (CF) similarly localized both woods. Their distribution from phloem evaluated by high‐performance liquid chromatography (HPLC) serial...
Lignin is a major component of plant cell walls and synthesised through oxidative polymerisation monolignols. The transcription level laccase, an enzyme implicated in monolignol polymerisation, higher the tissue forming compression wood than normal wood. Compression wood, which special xylem that develops to reorient inclined stems, also has lignin content In present study, Chamaecyparis obtusa Endl. saplings were grown on incline following variables tracked for 10 weeks: posture recovery...
The transverse section of compression wood tracheids has a circular shape and intercellular spaces. cause not been determined yet; however, we hypothesized that peeling the cell wall adhesion would cellular intervals, resulting in circularity tracheids. Homogalacturonan, type pectin, functions adhesion. Further, pectin methylesterase (PME) is involved functionalization homogalacturonan. We quantitated PME gene expression levels differentiating xylem cells using different degrees samples...