A5029943434- Antibiotic Resistance in Bacteria
- Brucella: diagnosis, epidemiology, treatment
- Salmonella and Campylobacter epidemiology
- Pharmaceutical and Antibiotic Environmental Impacts
- Microbial infections and disease research
- Veterinary medicine and infectious diseases
- Marine animal studies overview
- Mycobacterium research and diagnosis
- Bacterial Identification and Susceptibility Testing
- Gut microbiota and health
- Burkholderia infections and melioidosis
- Galectins and Cancer Biology
- Bacteriophages and microbial interactions
- Escherichia coli research studies
- Genomics and Phylogenetic Studies
- Humic Substances and Bio-Organic Studies
- Pneumonia and Respiratory Infections
- Animal Diversity and Health Studies
- Identification and Quantification in Food
- Antimicrobial Resistance in Staphylococcus
- Aquaculture disease management and microbiota
- Advanced machining processes and optimization
- Animal Disease Management and Epidemiology
- Plant Pathogenic Bacteria Studies
- Streptococcal Infections and Treatments
Animal and Plant Health Agency
2011-2024
Veterinary Medicines Directorate
2006-2010
To determine the occurrence of mcr-1 and mcr-2 genes in Gram-negative bacteria isolated from healthy pigs Great Britain. (n = 657) between 2014 2015 were examined by WGS. Variants identified Moraxella spp. pooled caecal contents at slaughter collected six farms Other bacteria, including Escherichia coli same farms, not detected harbouring or mcr-2. A porci-like isolate, MSG13-C03, harboured MCR-1.10 with 98.7% identity to MCR-1, we describe first detection mcr-6.1, found on chromosome...
Illumina sequencing allows rapid, cheap and accurate whole genome bacterial analyses, but short reads (<300 bp) do not usually enable complete assembly. Long-read greatly assists with resolving complex genomes, particularly when combined short-read data (hybrid assembly). However, it is clear how different long-read methods affect hybrid assembly accuracy. Relative automation of the process also crucial to facilitating high-throughput reconstruction, avoiding multiple bespoke filtering...
ABSTRACT Naturally acquired infection of humans with a marine mammal-associated Brucella sp. has only been reported once previously in study describing infections two patients from Peru. We report the isolation and characterization strain New Zealand patient that appears most closely related to strains identified mammals. The isolate was preliminarily as suis using conventional bacteriological tests our laboratory. However, results profile not an exact match, forwarded four international...
The pangenome of Escherichia coli from nearby farms shows how livestock host and local geography shape bacterial populations.
ABSTRACT Illumina sequencing allows rapid, cheap and accurate whole genome bacterial analyses, but short reads (<300 bp) do not usually enable complete assembly. Long read greatly assists with resolving complex genomes, particularly when combined short-read data (hybrid assembly). However, it is clear how different long-read methods impact on assembly accuracy. Relative automation of the process also crucial to facilitating high-throughput reconstruction, avoiding multiple bespoke...
Combatting antimicrobial resistant (AMR) using a One-Health approach is essential as various bacteria, including Escherichia coli , common are becoming increasingly and livestock may be reservoir. The AMR gene content of 492 E. isolated from 56 pig farms across Great Britain in 2014–2015, purified on antibiotic selective non-selective plates, was determined whole genome sequencing (WGS). were phylogenetically diverse harboring variety profiles with widespread resistance to “old” antibiotics;...
Background Surveillance of commensal Escherichia coli , a possible reservoir antimicrobial resistance (AMR) genes, is important as they pose risk to human and animal health. Most surveillance activities rely on phenotypic characterisation, but whole genome sequencing (WGS) presents an alternative. Aim In this retrospective study, we tested 515 E. isolated from pigs evaluate the use WGS predict phenotype. Methods Minimum inhibitory concentration (MIC) was determined for nine antimicrobials...
Enterobacterales from livestock are potentially important reservoirs for antimicrobial resistance (AMR) to pass through the food chain humans, thereby increasing AMR burden and affecting our ability tackle infections. In this study 168 isolates four genera of order , primarily Escherichia coli were purified (cattle, pigs sheep) faeces 14 farms in United Kingdom. Their genomes resolved using long- short-read sequencing analyse genes their genetic context, as well explore relationship between...
There are concerns that antimicrobial usage (AMU) is driving an increase in multi-drug resistant (MDR) bacteria so treatment of microbial infections becoming harder humans and animals. The aim this study was to evaluate factors, including usage, affect resistance (AMR) on farm over time.A population 14 cattle, sheep pig farms within a defined area England were sampled three times year collect data AMR faecal Enterobacterales flora; AMU; husbandry or management practices. Ten pooled samples...
Extension of known ecological niches Brucella has included the description two novel species from marine mammals. pinnipedialis is associated predominantly with seals, while major ceti clades, most commonly porpoises or dolphins respectively, have been identified. To date there limited characterisation isolates obtained mammals outside Northern European waters, including North American waters. address this gap, and extend knowledge global population structure host associations these species,...
F-type plasmids are diverse and of great clinical significance, often carrying genes conferring antimicrobial resistance (AMR) such as extended-spectrum β-lactamases, particularly in Enterobacterales. Organising this plasmid diversity is challenging, current knowledge largely based on from settings. Here, we present a network community analysis large survey environmental (influent, effluent upstream/downstream waterways surrounding wastewater treatment works) livestock We use tractable...
Introduction: Brucellosis is a zoonotic disease that has significant economic, social and public health impact in many parts of the world. The causative agents are members genus Brucella currently comprising 11 species with an expanding known host range recent years. Case presentation: One pair White’s tree frogs ( Litoria caerulea ) developed skin lesions from which pure growth haemolytic organism was obtained. isolate identified as melitensis by matrix‐assisted laser desorption/ionization...
Brachyspira hyodysenteriae is the aetiological agent of swine dysentery, a globally distributed disease that causes profound economic loss, impedes free trade and movement animals, has significant impact on pig health. Infection generally treated with antibiotics which pleuromutilins, such as tiamulin, are widely used for this purpose, but reports resistance worldwide threaten continued effective control. In pleuromutilin been associated mutations in chromosomal genes encoding...
Bacteria of the genus Brucella are causative organisms brucellosis in animals and man. Previous characterisation strains originating from marine mammals showed them to be distinct terrestrial species likely comprise one or more new taxa. Recently two comprising isolates mammals, B. pinnipedialis ceti, were validly published. Here we report on an extensive study molecular phenotypic characteristics mammal how these relate newly described species.In this study, 102 eleven characterised....
Adult female nematodes identified as Pseudalius inflexus were collected from the lungs of a juvenile male harbor porpoise (Phocoena phocoena) found dead on beach in Cornwall, UK. Classic and molecular typing methods, immunologic electron microscopy immunolabeling techniques, provided evidence Brucella sp. infection within uterine tissue this marine mammal. This finding presents further to suggest parasites should be considered potential means transfer bacterial mammals highlights zoonotic...
ABSTRACT Brucella taxonomy is perpetually being reshuffled, at both the species and intraspecies levels. Biovar 7 of abortus was suspended from Approved Lists Bacterial Names classification in 1988, because unpublished evidence that reference strain 63/75 a mixture B. biovars 3 5. To formally clarify situation, all isolates previously identified as bv. AHVLA ANSES collections were characterized by classical microbiological multiple molecular approaches. Among 14 investigated strains,...
Brucella ceti is an emerging zoonotic pathogen that has been recovered from several species of cetaceans in the world's oceans over past 20 yr. We report recovery B. a Sowerby's beaked whale (Mesoploden bidens) and long-finned pilot (Globicehala melas). Recovery testis provides further evidence potential for infection to impact reproductive success cetaceans, many which are threatened species. The addition another two cetacean growing number also emphasizes concern human infections with this...
Eight bottlenose dolphins ( Tursiops truncatus ) that stranded in Cornwall, south‐west England, between June 2004 and December 2007 were examined using standardised postmortem examination bacteriological methods. Evidence of Brucella species infection was found four these on culture. In addition, the eight dolphins, positive two weakly for antibodies to serological analyses pericardial other fluids a competitive ELISA indirect ELISAs. High or very high levels sum 25 individual chlorobiphenyl...
Introduction Surveillance is vital for monitoring the increasing risk of antimicrobial resistance (AMR) in bacteria leading to failures humans and animals treat infections. In a One Health context, AMR from livestock food can transfer through chain humans, vice versa, which be characterized detail genomics. We investigated critical aspects dynamics poultry UK. Methods this study, we performed whole genome sequencing genomic characterization 761 extended-spectrum cephalosporinases (ESCs)...
DAO Diseases of Aquatic Organisms Contact the journal Facebook Twitter RSS Mailing List Subscribe to our mailing list via Mailchimp HomeLatest VolumeAbout JournalEditorsSpecials 126:13-23 (2017) - DOI: https://doi.org/10.3354/dao03163 Exposure harbour seals Phoca vitulina Brucella in declining populations across Scotland Joanna L. Kershaw1,*, Emma J. Stubberfield2, Geoffrey Foster3, Andrew Brownlow3, Ailsa Hall1, Lorraine Perrett2 1Sea Mammal Research Unit, Scottish Oceans Institute,...
A monophasic group B Salmonella enterica 4,12:a:‐ was first isolated in harbour porpoises ( Phocoena phocoena ) Scotland 1991. This paper reports the isolation of same S from porpoise carcases found stranded along Cornwall and Devon coastlines. Between 1991 2002, 80 were submitted for postmortem examination subjected to bacteriological under UK Cetacean Strandings Investigation Programme. total 28 isolates recovered several tests, including biochemical, molecular serological analysis.
Brachyspira hyodysenteriae and pilosicoli cause economically important enteric disease in pigs. Treatment of these infections often includes antimicrobial administration, which can be most effective when therapeutic options are informed by susceptibility testing data. Here we describe a method for broth dilution bacteria, both difficult to culture vitro. The protocol was evaluated its fitness use an inter-laboratory ring trial involving eight laboratories from seven countries, employing...