A5056410179- Brucella: diagnosis, epidemiology, treatment
- Salmonella and Campylobacter epidemiology
- Burkholderia infections and melioidosis
- Streptococcal Infections and Treatments
- Escherichia coli research studies
- Bacteriophages and microbial interactions
- Animal Diversity and Health Studies
- Microbial infections and disease research
- Galectins and Cancer Biology
- Antimicrobial Resistance in Staphylococcus
- Infective Endocarditis Diagnosis and Management
- Neonatal and Maternal Infections
- Animal Disease Management and Epidemiology
- Pneumonia and Respiratory Infections
- Rabies epidemiology and control
- Genomics and Phylogenetic Studies
- Bacterial Identification and Susceptibility Testing
- Viral gastroenteritis research and epidemiology
- Bacterial Infections and Vaccines
- Monoclonal and Polyclonal Antibodies Research
- Mycobacterium research and diagnosis
- Yersinia bacterium, plague, ectoparasites research
- HIV Research and Treatment
- Marine animal studies overview
- Viral Infections and Vectors
Animal and Plant Health Agency
2015-2024
Veterinary Medicines Directorate
2006-2021
Inserm
2011
Université de Montpellier
2011
University of Warwick
1997-2009
National Public Health Laboratory
2001
Freeman Hospital
2000
Public Health Laboratory
2000
North Tyneside General Hospital
2000
Defence Science and Technology Laboratory
1994-1998
Two Gram-negative, non-motile, non-spore-forming, coccoid bacteria (strains CCM 4915T and 4916), isolated from clinical specimens of the common vole Microtus arvalis during an epizootic in Czech Republic 2001, were subjected to a polyphasic taxonomic study. On basis 16S rRNA (rrs) recA gene sequence similarities, both isolates allocated genus Brucella. Affiliation Brucella was confirmed by DNA–DNA hybridization studies. Both strains reacted equally with M-monospecific antiserum lysed...
A Gram-negative, non-motile, non-spore-forming coccoid bacterium (strain BO1(T)) was isolated recently from a breast implant infection of 71-year-old female patient with clinical signs brucellosis. Affiliation strain BO1(T) to the genus Brucella confirmed by means polyamine pattern, polar lipid profile, fatty acid quinone system, DNA-DNA hybridization studies and insertion sequence 711 (IS711)-specific PCR. Strain harboured four five copies Brucella-specific element IS 711, displaying unique...
Two Gram-negative, non-motile, non-spore-forming coccoid bacteria (strains F8/08-60 T and F8/08-61) isolated from clinical specimens obtained baboons ( Papio spp.) that had delivered stillborn offspring were subjected to a polyphasic taxonomic study. On the basis of 16S rRNA gene sequence similarities, both strains, which possessed identical sequences, assigned genus Brucella . This placement was confirmed by extended multilocus analysis (MLSA), where strains whole-genome sequencing...
Two slow-growing, Gram-negative, non-motile, non-spore-forming, coccoid bacteria (strains F60T and F965), isolated in Austria from mandibular lymph nodes of two red foxes (Vulpes vulpes), were subjected to a polyphasic taxonomic analysis. In recent study, both isolates assigned the genus Brucella but could not be attributed any existing species. Hence, we have analysed strains further detail determine their exact position genetic relatedness other members Brucella. The genome sizes F965 3...
The oral streptococcal group (mitis phylogenetic group) currently consists of nine recognized species, although the has been traditionally difficult to classify, with frequent changes in nomenclature over years. pneumococcus (Streptococcus pneumoniae), an important human pathogen, is distinguished from most closely related species Streptococcus mitis and oralis on basis three differentiating characteristics: optochin susceptibility, bile solubility, agglutination antipneumococcal...
The effects of hypersaline treatment (osmotic upshock) on solute accumulation have been studied in the Gram-positive bacterium Bacillus subtilis. Natural abundance 13C NMR spectroscopy studies revealed only proline as a major organic osmoticum cells grown defined medium (no exogenous solutes) and this finding was confirmed by amino acid analysis. Intracellular concentrations both K+ rose markedly after osmotic upshock. influx from rapid (< 1 h) but synthesis slower process (5–9 h). Proline...
Streptococcus suis is an important pathogen of pigs and occasionally causes serious human disease. However, little known about the S. population structure, clonal relationships between strains, potential particular clones to cause disease, relevance serotype as a marker for epidemiology. Here we describe multilocus sequence typing (MLST) scheme developed in order begin address these issues. Seven housekeeping gene fragments from each 294 isolates obtained various diseases asymptomatic...
Brucella species include economically important zoonotic pathogens that can infect a wide range of animals. There are currently six classically recognised although, as yet unnamed, isolates from various marine mammal have been reported. In order to investigate genetic relationships within the group and identify potential diagnostic markers we sequenced multiple loci large sample representing known diversity genus. Nine discrete genomic corresponding 4,396 bp sequence were examined 160...
Streptococcus suis is a zoonotic pathogen that infects pigs and can occasionally cause serious infections in humans. S. occur sporadically human Europe North America, but recent major outbreak has been described China with high levels of mortality. The mechanisms pathogenesis humans are poorly understood.
The effects of hypersaline treatment (osmotic upshock) on cell water relations were examined in the Gram-positive bacterium Bacillus subtilis using particle size analysis. Application Boyle-van't Hoflf relationship (cell volume versus reciprocal external osmolality) permitted direct determination turgor pressure, which was approximately 0·75 osmol kg−1 (1·9 MPa) exponentially growing bacteria a defined medium. abolition pressure immediately after upshock and subsequent recovery...
ABSTRACT Naturally acquired infection of humans with a marine mammal-associated Brucella sp. has only been reported once previously in study describing infections two patients from Peru. We report the isolation and characterization strain New Zealand patient that appears most closely related to strains identified mammals. The isolate was preliminarily as suis using conventional bacteriological tests our laboratory. However, results profile not an exact match, forwarded four international...
An extended multilocus sequence analysis (MLSA) scheme applicable to the Brucella, an expanding genus that includes zoonotic pathogens severely impact animal and human health across large parts of globe, was developed. The scheme, which extends a previously described 9 locus by examining sequences at 21 independent genetic loci in order increase discriminatory power, applied globally temporally diverse collection over 500 isolates representing all twelve known Brucella species providing...
Brucella species include important zoonotic pathogens that have a substantial impact on both agriculture and human health throughout the world. Brucellae are thought of as "stealth pathogens" escape recognition by host innate immune response, modulate acquired evade intracellular destruction. We analyzed genome sequences members family Brucellaceae to assess its evolutionary history from likely free-living soil-based progenitors into highly successful pathogens. Phylogenetic analysis split...
Bacteria of the genus Brucella are facultative intracellular parasites that cause brucellosis, a severe animal and human disease. Recently, group taxonomists merged brucellae with primarily free-living, phylogenetically related
Experimental evidence from the simian immunodeficiency virus (SIV) model of AIDS has shown that nef gene is critical in pathogenesis AIDS. Consequently, considerable interest both antiviral drug and vaccine development. Preliminary findings two rhesus macaques indicated a deletion only 12 bp found overlapping nef/3' long terminal repeat (LTR) region (9501 to 9512) SIVmacC8 molecular clone was associated with reduced isolation frequency. We show this can be repaired vivo by sequence...
ABSTRACT Members of the genus Brucella infect many domesticated and wild animals cause serious zoonotic infection in humans. The availability discriminatory molecular typing tools to inform assist conventional epidemiological approaches would be invaluable controlling these infections, but efforts have been hampered by genetic homogeneity genus. We report here on a subtyping system based 21 variable-number tandem-repeat (VNTR) loci consisting 13 previously unreported 8 reported elsewhere....
ABSTRACT The com operon of naturally transformable streptococcal species contains three genes, comC , comD and comE involved in the regulation competence. gene encodes a competence-stimulating peptide (CSP) thought to induce competence bacterial population at critical extracellular concentration. genes are believed encode transmembrane histidine kinase response regulator proteins, respectively, two-component regulator, with -encoded protein being receptor for CSP. Here we report on genetic...
Summary To examine the molecular population genetics of M protein family Streptococcus pyogenes (group A Streptococcus) , 5′ regions polymerase chain reaction‐amplified emm products from 79 serotypes were sequenced and phylogeny was compared to estimates overall genetic relationships among strains determined by multilocus enzyme electrophoresis. Although sequences several designated as distinct types identical or almost identical, pattern is characterized very extensive variation. The...