A5101782929- Alzheimer's disease research and treatments
- Complement system in diseases
- Neuroscience and Neuropharmacology Research
- Parkinson's Disease Mechanisms and Treatments
- Platelet Disorders and Treatments
- Renal Diseases and Glomerulopathies
- Metabolomics and Mass Spectrometry Studies
- Cholinesterase and Neurodegenerative Diseases
- Neuroinflammation and Neurodegeneration Mechanisms
- Computational Drug Discovery Methods
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Nuclear Receptors and Signaling
- Lymphoma Diagnosis and Treatment
- Blood groups and transfusion
- Pancreatic and Hepatic Oncology Research
- Prion Diseases and Protein Misfolding
- Immune cells in cancer
- Pediatric Urology and Nephrology Studies
- Pain Management and Opioid Use
- Trypanosoma species research and implications
- Hemoglobinopathies and Related Disorders
- Cervical and Thoracic Myelopathy
- Lung Cancer Diagnosis and Treatment
- Urinary Tract Infections Management
- Glycosylation and Glycoproteins Research
Beijing University of Chinese Medicine
2024-2025
Sun Yat-sen University
2024
Brigham and Women's Hospital
2011-2023
Harvard University
2011-2023
University of Illinois Chicago
2017-2021
BioLegend (United States)
2020-2021
The Ohio State University Wexner Medical Center
2006-2018
Helmholtz Zentrum München
2017
The Ohio State University
2005-2016
Wonkwang University
2014
Alzheimer disease is a major cause of cognitive failure, and pathogenically related but more subtle process accounts for many cases mild memory symptoms in older humans. Insoluble fibrillar plaques amyloid β-proteins (Aβ) neurofibrillary deposits hyperphosphorylated tau proteins are the diagnostic lesions AD, their temporal mechanistic relationship has long been debated. The recent recognition that small, diffusible oligomers may be principal bioactive form Aβ raises key question whether...
In Alzheimer's disease (AD), dementia severity correlates strongly with decreased synapse density in hippocampus and cortex. Numerous studies report that hippocampal long-term potentiation (LTP) can be inhibited by soluble oligomers of amyloid β-protein (Aβ), but the synaptic elements mediate this effect remain unclear. We examined field EPSPs whole-cell recordings wild-type mouse slices. Soluble Aβ from three distinct sources (cultured cells, AD cortex, or synthetic peptide) LTP, was...
Copy number mutations implicate excess production of α-synuclein as a possibly causative factor in Parkinson's disease (PD). Using an unbiased screen targeting endogenous gene expression, we discovered that the β2-adrenoreceptor (β2AR) is regulator (SNCA). β2AR ligands modulate SNCA transcription through histone 3 lysine 27 acetylation its promoter and enhancers. Over 11 years follow-up 4 million Norwegians, agonist salbutamol, brain-penetrant asthma medication, was associated with reduced...
Complement components and their receptors are found within around amyloid β (Aβ) cerebral plaques in Alzheimer's disease (AD). Microglia defend against pathogens through phagocytosis via complement component C3 and/or engagement of cleavage product iC3b with receptor type 3 (CR3, Mac-1). Here, we provide direct evidence that Mac-1 mediate, part, clearance fibrillar amyloid-β (fAβ) by murine microglia vitro vivo. took up not only synthetic fAβ(42) but also cores from patients AD, transporting...
Recent evidence suggests that tau aggregation may spread via extracellular release and subsequent uptake by synaptically connected neurons, but little is known about the processes which released or molecular forms of tau. To gain insight into nature tau, we used highly sensitive ELISAs, which, when in tandem, are capable differentiating between full-length (FL) mid-region-bearing fragments, C-terminal (CT) fragments. We applied these assays to systematic study conditioned media N2a cells,...
Idiopathic thrombotic thrombocytopenic purpura (TTP) is characterized by frequent recurrences. Effective screening for relapses will enable intervention prior to overt episodes of TTP. The present study used a modified assay detect ADAMTS13 (a disintegrin and metalloprotease with thrombospondin type 1 motif, 13) activity as low 0.5%. This analytical improvement permits adequate measurement levels in 97% remission samples statistical modelling. antibody (IgG) were measured 157 serial...
Elevated temperature and reduced water availability are frequently linked abiotic stresses that may provoke distinct as well interacting molecular responses. Based on non-targeted metabolomic transcriptomic measurements from Arabidopsis rosettes, this study aims at a systematic elucidation of relevant components in different drought heat scenarios relationships between players stress response. In combined drought-heat stress, the majority single responses maintained. However, interaction...
Abstract The mechanisms by which parkin protects the adult human brain from Parkinson disease remain incompletely understood. We hypothesized that cysteines participate in redox reactions and these are reflected its posttranslational modifications. found post mortem brain, including Substantia nigra , is largely insoluble after age 40 years; this transition linked to oxidation, such as at residues Cys95 Cys253. In mice, oxidative stress induces modifications of lower solubility vivo....
Pharmacogenomics, the study on genetic contributions to drug action may help in certifying fentanyl toxicity. Fentanyl is used clinically as an adjunct surgical anesthesia and for chronic pain management. Its toxicity be partially due cytochrome P450 (CYP) 3A4*1B 3A5*3 variant alleles, resulting variable metabolism. In this study, we examined 25 fentanyl-related deaths (22 Caucasians, 1 African-American, 2 Native-Americans) from Milwaukee County Medical Examiner's Office referral cases....
Abstract Although the amyloid β-protein (Aβ) is believed to play an initiating role in Alzheimer’s disease (AD), molecular characteristics of key pathogenic Aβ forms are not well understood. As a result, it has proved difficult identify optimal agents that target disease-relevant Aβ. Here, we combined use Aβ-rich aqueous extracts brain samples from AD patients as source human and live-cell imaging iPSC-derived neurons develop bioassay capable quantifying relative protective effects multiple...
Pathologic, biochemical and genetic evidence indicates that accumulation aggregation of amyloid β-proteins (Aβ) is a critical factor in the pathogenesis Alzheimer's disease (AD). Several therapeutic interventions attempting to lower Aβ have failed ameliorate cognitive decline patients with clinical AD significantly, but most such approaches target only one or two facets production/clearance/toxicity do not consider heterogeneity human species. As synaptic dysfunction may be among earliest...
Summary We present the results of two consecutively performed cohort studies that evaluated clinical effects corticosteroids or ciclosporin as an adjunct to plasma exchange (PE) for treatment acute episode thrombotic thrombocytopenic purpura (TTP). In comparing 12 corticosteroid‐treated patients with eight ciclosporin‐treated patients, none suffered exacerbation recurrence TTP in first 30 d after discontinuing PE compared 6/10 (60%) ( P = 0·042). These data suggest may have advantages over...
Summary We hypothesized that ciclosporin (CSA) as adjunct to plasma exchange (PE) improves the efficacy of PE in idiopathic thrombotic thrombocytopenic purpura (TTP) via suppression antibody inhibitor ADAMTS13. Our preliminary findings with CSA and upfront treatment TTP suggested addition significantly decreased exacerbation (disease recurrence within 30 d last PE) rates compared a cohort received corticosteroids their therapy TTP. present an updated analysis long‐term follow‐up 18 patients...
Alveolar macrophages (AMs) are a subset of tissue situated in the alveolar milieu. Compared with their precursor blood monocytes, AMs exhibit distinct physiologic functions unique to anatomic location. However, molecular details that control monocyte differentiation into remain unknown. This study employed proteomic approach define protein characteristics distinguish from monocytes. and monocytes were obtained six nonsmoking, healthy donors. Whole cell lysates each donor's analyzed by...
Background The assay for ADAMTS13 activity helps clinicians to confirm the clinical diagnosis of idiopathic thrombotic thrombocytopenic purpura. value testing antigen level protein is, however, less clear.Design and Methods In this study, both levels were measured in 835 sequential samples from 40 consecutive patients who followed an average 29 months throughout course acute episode plasma exchange treatment remission.Results During episodes, was severely deficient while more variable,...