A5080986767- Single-cell and spatial transcriptomics
- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Neonatal Respiratory Health Research
- Veterinary Medicine and Surgery
- Congenital Diaphragmatic Hernia Studies
- Microfluidic and Bio-sensing Technologies
- Parvovirus B19 Infection Studies
- Pluripotent Stem Cells Research
- Lymphoma Diagnosis and Treatment
- CAR-T cell therapy research
- Prenatal Screening and Diagnostics
- Reproductive Physiology in Livestock
- Cancer Immunotherapy and Biomarkers
- Peatlands and Wetlands Ecology
- Heart Rate Variability and Autonomic Control
- Congenital heart defects research
- Scientific Computing and Data Management
- Cardiovascular Function and Risk Factors
- Cardiovascular and exercise physiology
- Kawasaki Disease and Coronary Complications
- Systemic Lupus Erythematosus Research
- ATP Synthase and ATPases Research
- Animal Behavior and Welfare Studies
- Vasculitis and related conditions
University of Chicago
2024
NorthShore University HealthSystem
2024
University of Rochester Medical Center
2008-2024
The Ohio State University
2022-2023
University of Rochester
2009-2018
Wellborn Road Veterinary Medical Center
2014
Human lung morphogenesis begins by embryonic life and continues after birth into early childhood to form a complex organ with numerous morphologically functionally distinct cell types. Pulmonary organogenesis involves dynamic changes in proliferation, differentiation, migration of specialized cells derived from diverse lineages. Studying the molecular cellular processes underlying formation fully functional requires isolating pulmonary populations during development. We now report novel...
Abstract Cell type-resolved proteome analyses of the brain, heart and liver have been reported, however a similar effort on lipidome is currently lacking. Here we applied liquid chromatography-tandem mass spectrometry to characterize major lung cell types isolated from human donors, representing first map any organ. We coupled this with proteomics same samples (available at Lungmap.net). Complementary substantiated functional identity cells. Lipidomics showed significant variations in across...
The purpose of this document is to define minimal standards for a flow cytometry shared resource laboratory (SRL) and provide guidance best practices in several important areas. This effort driven by the desire International Society Advancement Cytometry (ISAC) members SRLs maintain excellence cytometry, act as repository key elements information (e.g. example SOPs/training material, etc.). These are not intended specifically how implement these recommendations, but rather establish goals an...
Cell sorting is a commonly used technology to isolate highly purified cell populations for downstream applications. Because the sorted cells are destined further analysis, i.e., gene expression assays or functional assays, ensuring that process itself has little effect on of utmost importance. Previous studies examining effects cellular function have primarily focused specific type condition. One goals Flow Cytometry Research Group Association Biomolecular Resource Facilities establish best...
Epizootic hemorrhagic disease virus (EHDV) causes a highly infectious noncontagious in wild and captive deer (Cervidae) populations the US. Although rapid accurate identification of is important, serotype equally important for understanding epidemiology white-tailed (Odocoileus virginianus) populations. We developed one-step multiplex reverse transcriptase PCR assay differentiation EHDV serotypes 1, 2, 6 cell culture clinical samples by targeting viral gene segment 2 (L2) that encodes...
Postnatal lung development results in an increasingly functional organ prepared for gas exchange and pathogenic challenges. It is achieved through cellular differentiation migration. Changes the tissue architecture during this process are well-documented increasing diversity associated with it reported recent years. Despite progress, transcriptomic molecular pathways human postnatal yet to be fully understood. In study, we investigated gene expression patterns healthy pediatric four major...
Aortitis is an uncommon cause of aortic aneurysms. Arterial inflammation can lead to irreversible vascular damage. Early recognition necessary for treatment prevent permanent consequences vessel inflammation.
Cell sorting is a commonly used technology to isolate highly purified cell populations for downstream applications. Because the sorted cells are destined further analysis,
A complete invasive hemodynamic assessment, including both standard and advanced parameters improves risk stratification in patients with heart failure. Specifically, the novel parameters, aortic pulsatility index (API) cardiac power output (CPO), have shown robust prognostic potential cardiogenic shock However, incremental benefit of a simultaneous utilization composite across spectrum left- right- sided dysfunction currently remains unknown. We performed retrospective study all failure...
Background: Cardiopulmonary exercise testing (CPET) remains an important test for risk assessment in ambulatory heart failure patients to identify those who may benefit from advanced therapies, such as left ventricular assist device (LVAD) or transplantation (HT). The prognostic strength of cardiac performance variables at peak exercise, including oxygen consumption (VO 2 ), pulse (O pulse) and circulatory power (CP) is known. We explored the utility augmentation these parameters between...
Endoscopic-assisted transcervical inseminations (TCIs) have become increasingly popular. The aim of this retrospective clinical study was to evaluate data from the performed at our facility. We evaluated January 2018 through December 2021. This included 137 cases with fresh, 67 chilled, and 63 using frozen-thawed semen. All bitches underwent breeding management determine ideal period. semen samples were for total number sperm, motility, progressive motility. Pregnancy determined by B-mode...