A5016445865- Immune Response and Inflammation
- Neonatal and fetal brain pathology
- Planarian Biology and Electrostimulation
- Neonatal Respiratory Health Research
- Spinal Cord Injury Research
- Neurogenesis and neuroplasticity mechanisms
- Spinal Dysraphism and Malformations
- Proteoglycans and glycosaminoglycans research
- Microbial Inactivation Methods
- Head and Neck Surgical Oncology
- Magnetic and Electromagnetic Effects
- Nerve injury and regeneration
- Mesenchymal stem cell research
Centro de Investigacion Principe Felipe
2019-2024
Hospital Nacional de Parapléjicos
2024
Université Paris-Saclay
2023-2024
Institut Gustave Roussy
2023-2024
Centre National de la Recherche Scientifique
2023-2024
Abstract Chondroitin sulfate proteoglycans (CSPGs) act as potent inhibitors of axonal growth and neuroplasticity after spinal cord injury (SCI). Here we reveal that CSPGs also play a critical role in preventing inflammation resolution by blocking the conversion pro-inflammatory immune cells to pro-repair phenotype rodent models SCI. We demonstrate enzymatic digestion CSPG glycosaminoglycans enhances cell clearance reduces protein gene expression profiles at key time points. Analysis...
Abstract Background Toll-like receptors (TLRs) represent critical effectors in the host defense response against various pathogens; however, their known function during development has also highlighted a potential role cell fate determination and neural differentiation. While glial cells precursor (NPCs) of spinal cord express both TLR2 TLR4, influence on self-renewal differentiation remains incompletely described. Methods TLR2, TLR4 knock-out wild type mice were employed for tissue analysis...
Spinal cord injuries (SCIs) result in the loss of sensory and motor function with massive cell death axon degeneration. We have previously shown that transplantation spinal cord-derived ependymal progenitor cells (epSPC) significantly improves functional recovery after acute chronic SCI experimental models, via neuronal differentiation trophic glial support. Here, we propose an improved procedure based on epSPC a tubular conduit hyaluronic acid containing poly (lactic acid) fibres creating...
Myelomeningocele (MMC) is a congenital birth defect of the spine and spinal cord, commonly treated clinically through prenatal or postnatal surgery by repairing unclosed canal. Having previously developed PLA/PCL polymer smart patch for this condition, we aim to further expand potential therapeutic options providing additional cellular biochemical support in addition its mechanical properties. Bone morphogenetic proteins (BMPs) are large class secreted factors that serve as modulators...
In the tissue regeneration field, stem cell transplantation represents a promising therapeutic strategy. To favor their implantation, proliferation and differentiation need to be controlled. Several studies have demonstrated that fate can controlled by applying continuous electric field stimulation. This study aims characterize effect of specific microsecond pulse stimulation (bipolar pulses 100 µs + µs, delivered for 30 min at an intensity 250 V/cm) induce increase in on mesenchymal cells...
In the tissue regeneration field, stem cell transplantation and control of their differentiation represent a promising therapeutic strategy. The second aspect can be achieved using electrical stimulation.
 This study aims to characterize effect microsecond stimulation on MSCs iNSCs, in context RISEUP FET-OPEN project (n. 964562) that seeks these cells for spinal cord injury treatments.
 Here, specific electric pulses stimulation, characterized by bipolar 100 µs + µs, delivered 30...
Abstract Background: Toll-Like Receptors (TLRs) represent critical effectors in the host defense response against various pathogens; however, their known function during development has also highlighted a potential role cell fate determination and neural differentiation. While glial cells precursor (NPCs) of spinal cord express both TLR2 TLR4, influence on self-renewal differentiation remains incompletely described. Methods: TLR2, TLR4 knock-out wild type mice were employed for tissue...