A5018911898- Muscle Physiology and Disorders
- Pluripotent Stem Cells Research
- Tissue Engineering and Regenerative Medicine
- 3D Printing in Biomedical Research
- Retinal Development and Disorders
- MicroRNA in disease regulation
- Extracellular vesicles in disease
- RNA Interference and Gene Delivery
- Silk-based biomaterials and applications
- Virus-based gene therapy research
- Developmental Biology and Gene Regulation
- Zebrafish Biomedical Research Applications
- CRISPR and Genetic Engineering
- Nerve injury and regeneration
- Epigenetics and DNA Methylation
- Cancer therapeutics and mechanisms
- Cellular Mechanics and Interactions
- Cardiomyopathy and Myosin Studies
- Neuroscience and Neural Engineering
- Hedgehog Signaling Pathway Studies
- Microtubule and mitosis dynamics
- Cancer-related Molecular Pathways
- Retinal Diseases and Treatments
- RNA Research and Splicing
- CAR-T cell therapy research
AstraZeneca (Sweden)
2024
Minneapolis Heart Institute Foundation
2016-2020
University of Minnesota
2016-2020
University of Trento
2014-2018
Neuroscience Institute
2014
Sapienza University of Rome
2008-2011
Istituto Pasteur
2008-2010
Institute of Molecular Biology and Pathology
2008
Three-dimensional (3D) porous scaffolds combined with therapeutic stem cells play vital roles in tissue engineering. The adult brain has very limited regeneration ability after injuries such as trauma and stroke. In this study, injectable 3D silk fibroin-based hydrogel encapsulated neural were developed, aiming at supporting regeneration. To improve the function of towards cells, fibroin was modified by an IKVAV peptide through covalent binding. Both unmodified hydrogels obtained,...
Highlights•Genome-wide analyses reveal gene expression changes during human skeletal myogenesis•CD54, integrin α9β1, and SDC2 identify PAX7-induced myogenic progenitors•CD54+α9β1+SDC2+ progenitors have long-term in vivo regenerative potential•CD54 alone allows for GMP-compatible purification of progenitorsSummaryPluripotent stem (PS)-cell-derived cell types hold promise treating degenerative diseases. However, PS differentiation is intrinsically heterogeneous; therefore, clinical translation...
Abstract Small extracellular vesicles (sEV) derived from various cell sources have been demonstrated to enhance cardiac function in preclinical models of myocardial infarction (MI). The aim this study was compare different sEV for repair and determine the most effective one, which nowadays remains limited. We comprehensively assessed efficacy obtained human primary bone marrow mesenchymal stromal cells (BM‐MSC), immortalized MSC (hTERT‐MSC), embryonic stem (ESC), ESC‐derived progenitor...
Different hydrogel materials have been prepared to investigate the effects of culture substrate on behaviour pluripotent cells. In particular, genipin-crosslinked gelatin–silk fibroin hydrogels different compositions prepared, physically characterized and used as substrates for Pluripotent cells cultured remained viable proliferated. Gelatin silk promoted proliferation in short long term, respectively. Moreover, blended were induced an epithelial ectodermal differentiation fate, instead...
Optimal cell-based therapies for the treatment of muscle degenerative disorders should not only regenerate fibers but provide a quiescent satellite cell pool ensuring long-term maintenance and regeneration. Conditional expression Pax3/Pax7 in differentiating pluripotent stem cells (PSCs) allows generation myogenic progenitors endowed with enhanced regenerative capacity. To identify molecular determinants underlying their potential, we performed transcriptome analyses these along primary from...
Sex chromosomes shape male (XY) - female (XX) differences in development and disease. These can be modelled vitro by comparing XY XX human induced pluripotent stem cells (hiPSCs). However, this system, inter-individual autosomal variation unstable X-dosage compensation confound identification of sex chromosomal effects. Here, we utilise chromosome loss XXY fibroblasts to generate hiPSCs that are autosomally isogenic exhibit stable compensation. We also create X-monosomic (XO) hiPSCs,...
Many mutations and deletions in the dystrophin gene, responsible for Duchenne muscular dystrophy (DMD), can be corrected at posttranscriptional level by skipping specific exons. Here we show that long-term benefit obtained dystrophic mouse model through use of adeno-associated viral vectors expressing antisense sequences: persistent exon skipping, rescue, functional were observed 74 weeks after a single systemic administration. The therapeutic was sufficient to preserve muscle integrity mice...
One promising approach for the gene therapy of Duchenne muscular dystrophy (DMD) is exon skipping. When thinking possible intervention on human, it very crucial to identify most appropriate antisense sequences able provide highest skipping efficiency. In this article, we compared 51 activity 10 different molecules, raised against splice junctions and/or exonic splicing enhancers (ESEs), expressed as part U1 small nuclear RNA (snRNA). The effectiveness each construct was tested in human DMD...
Reversine is a potent antitumor 2,6-diamino-substituted purine acting as an Aurora kinases inhibitor and interfering with cancer cell cycle progression. In this study we describe three reversine-related molecules, designed by docking calculation, that present structural modifications in the diamino units at positions 2 6. We investigated conformations of most stable prototropic tautomers one these N6-cyclohexyl-N6-methyl-N2-phenyl-7H-purine-2,6-diamine (3), Density Functional Theory (DFT)...
Vertebrates share common mechanisms in the control of development and maintenance neural retinal function. The secreted factor Noggin, a BMP inhibitor, plays crucial role induction during embryonic development. Moreover, we have shown its involvement differentiation pluripotent cells. Here show Noggin expression adult retina three vertebrate species. Four genes are present zebrafish (Danio rerio; ZbNog1, 2, 3, 5), frog (Xenopus laevis; XenNog1, 2 4), one mouse (Mus musculus; mNog)....
It has long been known that the depletion of bone morphogenetic protein (BMP) is one key factors necessary for development anterior neuroectodermal structures. However, precise molecular mechanisms underlie forebrain regionalization are still not completely understood. Here, we show Noggin1 involved in neural structures a dose-dependent manner. Low doses expand prosencephalic territories, while higher specify diencephalic and retinal regions at expense telencephalic areas. A similar...
Embryonic stem cells (ESCs) can give rise to all the differentiated cell types of organism, including neurons. However, efficiency and specificity neural differentiation protocols still needs be improved in order plan their use replacement therapies. In this study, we modified a monolayer protocol by selecting green fluorescent protein (GFP) positive precursors with fluorescence-activated sorting (FACS). The enhancement was obtained positively for precursors, while specific neuronal subtypes...
Abstract Background Skeletal muscle function is essential for health, and it depends on the proper activity of myofibers their innervating motor neurons. Each adult composed different types with distinct contractile metabolic characteristics. The balance myofiber disrupted in most degenerative disorders, representing another factor compromising function. One promising therapeutic approach treatment these diseases cell replacement based targeted differentiation pluripotent stem cells (PSCs)...