A5014465631- Insect Resistance and Genetics
- Insect symbiosis and bacterial influences
- Entomopathogenic Microorganisms in Pest Control
- Insect and Pesticide Research
- Metabolism and Genetic Disorders
- Amino Acid Enzymes and Metabolism
- Enzyme Production and Characterization
- Biofuel production and bioconversion
- Chemical Reactions and Isotopes
- Insect-Plant Interactions and Control
- Anaerobic Digestion and Biogas Production
- Forest Insect Ecology and Management
- Studies on Chitinases and Chitosanases
- Insect Pest Control Strategies
- Yersinia bacterium, plague, ectoparasites research
- Bacteriophages and microbial interactions
- Antimicrobial Peptides and Activities
- Peptidase Inhibition and Analysis
- Enzyme Structure and Function
- Water resources management and optimization
AgResearch
2013-2022
Methane is a potent greenhouse gas, 25 times more efficient at trapping heat than carbon dioxide. Ruminant methane emissions contribute almost 30% to anthropogenic sources of global atmospheric levels and reduction in would significantly slowing temperature rises. Here we demonstrate the use lytic enyzme, PeiR, from methanogen virus that infects Methanobrevibacter ruminantium M1 as an effective agent inhibiting range rumen strains pure culture. We determined substrate specificity soluble...
ABSTRACT A highly virulent Serratia proteamaculans strain, AGR96X, exhibiting specific pathogenicity against larvae of the New Zealand grass grub ( Costelytra giveni ; Coleoptera: Scarabaeidae) and manuka beetle Pyronota festiva P. setosa Scarabaeidae), was isolated from a diseased larva. 12-day median lethal dose 4.89 × 10 3 ± 0.92 cells per larva defined for death occurred within 5 to 12 days following ingestion high bacterial dose. During infection period, bacterium rapidly multiplied...
Here we report the draft genome of Yersinia entomophaga type strain MH96T. The shows 93.8% nucleotide sequence identity to that nurmii APN3a-cT, and comprises a single chromosome approximately 4,275,531 bp. In silico analysis identified that, in addition previously documented Y. Yen-TC gene cluster, encodes diverse array toxins, including two III secretion systems, five rhs-associated clusters. As well as these multicomponent several orthologs known insect such VIP2 toxin binary PirAB,...
The bacterium Yersinia entomophaga is pathogenic to a range of insect species, with death typically occurring within 2 5 days ingestion. Per os challenge larvae the greater wax moth (Galleria mellonella) confirmed that Y. was virulent when fed held at 25°C but avirulent maintained 37°C. At 25°C, dose ~4 × 10(7) CFU per larva toxin complex (Yen-TC) deletion derivative, ΔTC variant, resulted in 27% mortality. This low level activity restored near-wild-type levels by augmentation diet sublethal...
Pseudomurein endoisopeptidases cause lysis of the cell walls methanogens by cleaving isopeptide bond Ala- ε -Lys in peptide chain pseudomurein. PeiW and PeiP are two thermostable pseudomurein encoded phage ΨM100 Methanothermobacter wolfei phages ΨM1 ΨM2 marburgensis , respectively. A continuous assay using synthetic substrates was developed used biochemical characterisation recombinant PeiP. The advantages these over natural sensitivity, high purity, fact that they more easily obtained than...
Abstract BACKGROUND Porina is the common name for moths and larvae of genus Wiseana (Lepidoptera: Hepialidae), some which are significant pasture pests in New Zealand. Because environmental concerns non‐target effects insecticide control measures, biological alternatives insect such as porina required. RESULTS Using a food preference assay time‐lapse photography, range low‐cost ingredients were assessed their palatability to larvae. Lead candidates combined into extruded bait variants,...
The grass grub endemic to New Zealand, Costelytra giveni (Coleoptera: Scarabaeidae), and the manuka beetle, Pyronota festiva P. setosa are prevalent pest species. Through assessment of bacterial strains isolated from diseased cadavers these insect species, 19 insect-active Serratia proteamaculans variants a single entomophila strain were isolated. When independently bioassayed, isolates differed in host range, rate disease progression, 12-day mortality rates, which ranged 60 100% challenged larvae. A
Brevibacillus laterosporus (Bl) is a Gram-positive and spore-forming bacterium belonging to the brevis phylogenetic cluster. Globally, insect pathogenic strains of have been isolated, characterised, some activities patented. Two isolates, Bl 1821L 1951, exhibiting pathogenicity against diamondback moth mosquitoes, are under development as biopesticide in New Zealand. However, due suspected activity putative antibacterial proteins (ABPs), endemic isolates often grow erratically. Various...
Methenyltetrahydromethanopterin cyclohydrolase (Mch) is involved in the methanogenesis pathway of archaea as a C1 unit carrier where N5-formyl-tetrahydromethanopterin converted to methenyl-tetrahydromethanopterin. Mch from Methanobrevibacter ruminantium was cloned, purified, crystallized and its crystal structure solved at 1.37 Å resolution. A biologically active trimer, enzyme composed two domains including an N-terminal domain six α-helices encompassing series four β-sheets predominantly...
Brevibacillus laterosporus (Bl) is a Gram-positive and spore-forming bacterium belonging to the brevis phylogenetic cluster. Globally, insect pathogenic strains of have been isolated, characterised, some activities patented. Two isolates, Bl 1821L 1951, exhibiting pathogenicity against diamondback moth mosquitoes, are under development as biopesticide in New Zealand. However, due suspected activity putative antibacterial proteins (ABPs), endemic isolates often grow erratically. Various...