A5012008379- Genomics and Chromatin Dynamics
- RNA Research and Splicing
- RNA modifications and cancer
- RNA and protein synthesis mechanisms
- Force Microscopy Techniques and Applications
- Plant Reproductive Biology
- Orbital Angular Momentum in Optics
- Mechanical and Optical Resonators
- RNA Interference and Gene Delivery
Ludwig-Maximilians-Universität München
2021-2024
How chromatin enzymes work in condensed and how they maintain diffusional mobility inside remains unexplored. We investigated these challenges using the
Single-molecule force spectroscopy using optical tweezers continues to provide detailed insights into the behavior of nanoscale systems. Obtaining precise measurements their mechanical properties is highly dependent on accurate instrument calibration. Therefore, instrumental drift or inaccurate calibration may prevent reaching an accuracy at theoretical limit and lead incorrect conclusions. Commonly encountered sources error include inaccuracies in detector sensitivity trap stiffness...
The genome is organized by diverse packaging mechanisms like nucleosome formation, loop extrusion and phase separation, which all compact DNA in a dynamic manner. Phase separation additionally drives protein recruitment to condensed sites thus regulates gene transcription. cohesin complex key player chromosomal organization that extrudes loops connect distant regions of the ensures sister chromatid cohesion after S-phase. For stable loading onto for activation, requires Scc2/4. As precise...