A5040565250- Malaria Research and Control
- Plant biochemistry and biosynthesis
- Research on Leishmaniasis Studies
- Coenzyme Q10 studies and effects
- Pharmacological Effects of Natural Compounds
- Mosquito-borne diseases and control
- Bioactive Compounds and Antitumor Agents
- Biochemical and Molecular Research
- Computational Drug Discovery Methods
- Trypanosoma species research and implications
- Synthesis and biological activity
- Microbial Natural Products and Biosynthesis
- COVID-19 impact on air quality
- Photosynthetic Processes and Mechanisms
- Vector-borne infectious diseases
- vaccines and immunoinformatics approaches
- Bacterial biofilms and quorum sensing
- Air Quality Monitoring and Forecasting
- Parasites and Host Interactions
- Amoebic Infections and Treatments
- Parasitic Infections and Diagnostics
- ATP Synthase and ATPases Research
Universidade de São Paulo
2018-2024
Institute of Biomedical Science
2020
A proposed treatment for malaria is a combination of fosmidomycin and clindamycin. Both compounds inhibit the methylerythritol 4-phosphate (MEP) pathway, parasitic source farnesyl geranylgeranyl pyrophosphate (FPP GGPP, respectively). FPP GGPP are crucial biosynthesis several essential metabolites such as ubiquinone dolichol, well protein prenylation. Dietary prenols, farnesol (FOH) geranylgeraniol (GGOH), can rescue parasites from MEP inhibitors, suggesting existence missing pathway prenol...
ABSTRACT Farnesol salvage, a two-step pathway converting farnesol to farnesyl pyrophosphate (FPP), occurs in bacteria, plants, and animals. This paper investigates the presence of this fungi. Through bioinformatics, biochemistry, physiological analyses, we demonstrate its absence yeasts Saccharomyces cerevisiae Candida albicans , suggesting likely across We screened 1,053 fungal genomes, including 34 from C. for potential homologs four genes ( Arabidopsis thaliana AtFOLK AtVTE5 AtVTE6...
Repurposing strategies present an enormous advantage for drug discovery, especially in malaria, where resources are scarce.
Leishmaniasis is a neglected disease caused by trypanosomatid protozoan of the genus Leishmania. Most drugs used to treat leishmaniasis are highly toxic, and emergence drug-resistant strains has been observed. Therefore, new therapeutic targets against required. Several isoprenoid compounds, including dolichols or ubiquinones, have shown be important for cell viability proliferation in various species. Here, we detected biosynthesis tocopherol Leishmania (L.) amazonensis promastigotes vitro...
Atovaquone (AV) acts on the malaria parasite by competing with ubiquinol (UQH 2 ) for its union to mitochondrial bc 1 complex, preventing ubiquinone-8 and ubiquinone-9 (UQ-8 UQ-9) redox recycling, which is a necessary step in pyrimidine biosynthesis. This study focused UQ biosynthesis Plasmodium falciparum adopted proof-of-concept research better elucidate mechanism of action AV improve efficacy.
One of the most controversial factors about malaria parasite culture is gaseous composition used. The commonly used one consists a mixture poor in O2 and rich CO2.The present study aimed to share standard methods from our research group simplifying Plasmodium falciparum cultures by employing atmospheric air (ATM) reusable glass bottles under agitation.Here, it was compared viability, free oxygen media, drug sensitivity between different strains isolates maintained for long periods ATM or...
Plasmodium falciparum is the etiological agent of human malaria, one most widespread diseases in tropical and subtropical regions. Drug resistance biggest problems controlling disease, which leads to need discover new antimalarial compounds. One promissory drugs purposed fosmidomycin, an inhibitor biosynthesis isoprene units by methylerythritol 4-phosphate (MEP) pathway, some cases failed clinical studies. Once formed, are condensed form longer structures such as farnesyl geranylgeranyl...
Abstract Ubiquinone (UQ) is a fundamental mitochondrial electron transport chain component. This compound synthesized as the condensation of p-substituted benzoic acid and polyisoprenic moiety catalyzed by enzyme 4-hydroxybenzoate polyprenyltransferase (EC 2.5.1.39). In Plasmodium spp., this still uncharacterized. work, we expressed sequence falciparum PF3D7_0607500 gene (abbreviated PfCOQ2) in coq2Δ mutant strain Saccharomyces cerevisiae, studied functionality its product. open reading...
Malaria is one of the most widespread parasitic diseases, especially in Africa, Southeast Asia and South America. One greatest problems for control disease emergence drug resistance, which leads to a need development new antimalarial compounds. The biosynthesis isoprenoids has been investigated as part strategy identify targets obtain drugs. Several isoprenoid quinones, including menaquinone-4 (MK-4/vitamin K2), α- γ-tocopherol ubiquinone (UQ) homologs UQ-8 UQ-9, were previously detected...
<p>The antiplasmodial activity assay was performed using a simple, high-sensitivity methodology based on nanoluciferase (nLuc)-transfected <i>P. falciparum </i>parasites. The results showed that some of the analogs were active at low micromolar concentration. most potent member series has S-farnesyl and triazole moiety substituted with methyl-naphtyl. cytotoxicity in eukaryotic cells provided good therapeutic indexes, being promising candidates for future antimalarial drugs...
The antiplasmodial activity assay was performed using a simple, high-sensitivity methodology based on nanoluciferase (nLuc)-transfected P. falciparum parasites. results showed that some of the analogs were active at low micromolar concentration. most potent member series has S-farnesyl and triazole moiety substituted with methyl-naphtyl. cytotoxicity in eukaryotic cells provided good therapeutic indexes, being promising candidates for future antimalarial drugs development. Our provide...
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Abstract A promising treatment for malaria is a combination of fosmidomycin and clindamycin. Both compounds inhibit the methylerythritol 4-phosphate (MEP) pathway, parasitic source farnesyl geranylgeranyl pyrophosphate (FPP GGPP, respectively). FPP GGPP are crucial biosynthesis several essential metabolites such as ubiquinone dolichol, well protein prenylation. Dietary prenols, farnesol (FOH) geranylgeraniol (GGOH), can rescue parasites from MEP inhibitors, suggesting existence missing...
Abstract Background: The most important tool for the study of malaria parasite is probably in vitro culture that was established 1976 by Trager and Jensen. However, methodology technically complex, expensive, unsuitable use laboratories regions with limited resources. Human factors such as hormones immune system are frequently ignored, similarity between conditions physiologic-like environment has been questioned. One controversial about Plasmodium gaseous composition employed , which...