A5056113807- Pancreatic function and diabetes
- 3D Printing in Biomedical Research
- Diabetes Management and Research
- Electrospun Nanofibers in Biomedical Applications
- Pluripotent Stem Cells Research
- Polymer Surface Interaction Studies
- Diabetes and associated disorders
- Cell Adhesion Molecules Research
- Immunotherapy and Immune Responses
- Mesenchymal stem cell research
- biodegradable polymer synthesis and properties
- Nanofabrication and Lithography Techniques
- Monoclonal and Polyclonal Antibodies Research
- Additive Manufacturing and 3D Printing Technologies
- Cellular Mechanics and Interactions
- Angiogenesis and VEGF in Cancer
- Tissue Engineering and Regenerative Medicine
- Surface Modification and Superhydrophobicity
- Nuclear Structure and Function
- Microfluidic and Bio-sensing Technologies
- Pancreatitis Pathology and Treatment
- Electrohydrodynamics and Fluid Dynamics
- Metabolism, Diabetes, and Cancer
- Innovative Microfluidic and Catalytic Techniques Innovation
- Advanced Sensor and Energy Harvesting Materials
McGill University
2016-2025
John Wiley & Sons (Germany)
2019
PROTEO
2013-2018
Effigis (Canada)
2018
Université Laval
2012-2015
University of British Columbia
2009-2014
Hôpital Saint-François d'Assise
2012-2013
Canada's Michael Smith Genome Sciences Centre
2009-2012
Upsher-Smith Laboratories (United Kingdom)
2011
Different bioinks have been used to produce cell-laden alginate-based hydrogel constructs for cell replacement therapy but some of these approaches suffer from issues with print quality, long-term mechanical instability, and bioincompatibility. In this study, new were developed grid-shaped stable integrity immunomodulating capacity. Integrity printability improved by including the co-block-polymer Pluronic F127 in alginate solutions. To reduce inflammatory responses, pectin a low degree...
Alginate has been used to protect transplanted pancreatic islets from immune rejection and as a matrix increase the insulin content of islet progenitor cells. The throughput alginate bead generation by standard extrusion external gelation method is limited rate droplet formation nozzles. emulsion internal scaleable alternative that with biological molecules microbial cells, but not mammalian We describe novel adaptation this process cell immobilization. After optimization, yielded 90 ± 2%...
Abstract Pluripotent stem cell (PSC)-derived insulin-producing cells are a promising source for diabetes cellular therapy. However, the efficiency of multi-step process required to differentiate PSCs towards pancreatic beta is variable between lines, batches and even within cultures. In adherent differentiation protocols, we observed spontaneous local clustering expressing elevated nuclear expression endocrine transcription factors, PDX1 NKX6.1. Since aggregation has previously been shown...
Islet transplantation and more recently stem cell-derived islets were shown to successfully re-establish glycemic control in people with type 1 diabetes under immunosuppression. These results achieved through intraportal infusion which leads early graft losses limits the capacity contain retrieve implanted cells case of adverse events. Extra-hepatic sites encapsulation devices have been developed address these challenges potentially create an immunoprotective or immune-privileged...
Abstract Type 1 diabetes (T1D) is an autoimmune disease that leads to the loss of insulin-producing beta cells. Bioartificial pancreas (BAP) or cell replacement strategies have shown promise in curing T1D and providing long-term insulin independence. Hypoxia (low oxygen concentration) may occur BAP devices due consumption at early stages after implantation damages cells, addition imposing limitations device dimensions when translating promising results from rodents humans. Finding ways...
Pancreatic adenocarcinoma is one of the most lethal cancers, yet it remains understudied and poorly understood. Hyperinsulinemia has been reported to be a risk factor pancreatic cancer, rapid rise hyperinsulinemia associated with obesity type 2 diabetes foreshadows in cancer incidence. However, actions insulin at various stages progression remain defined. Here, we examined effects range doses on signalling, proliferation survival three human cell models meant represent progression: primary...
Abstract Encapsulation of insulin‐producing cells in alginate beads could improve the treatment type 1 diabetes by reducing or eliminating need for immunosuppression. We have recently adapted an emulsion and internal gelation process to β‐cell encapsulation. This has advantages being well suited m 3 /h production rates allowing use increased concentrations. Compared with 1.5% generated a standard extrusion process, 5% emulsion‐generated demonstrated greater vitro stability volumetric...
Abstract Gel‐matrix culture environments provide tissue engineering scaffolds and cues that guide cell differentiation. For many cellular therapy applications such as for the production of islet‐like clusters to treat Type 1 diabetes, need large‐scale can be anticipated. The throughput commonly used nozzle‐based devices encapsulation is limited by rate droplet formation ∼0.5 L/h. This work describes a novel process larger‐scale batch immobilization mammalian cells in alginate‐filled hollow...
Surface endothelialization could improve the long-term performance of vascular grafts and stents. We previously demonstrated that aerosol-generated fibronectin-derived peptide micropatterns consisting GRGDS spots over a WQPPRARI background increase endothelial cell yields in static cultures. developed novel fluorophore-tagged RGD (RGD-TAMRA) to visualize cell–surface interactions real-time. Here, we studied dynamics response laminar flow on these peptide-functionalized surfaces. Endothelial...
Cell microencapsulation is a promising approach to improve cell therapy outcomes by protecting injected cells from rapid dispersion and allowing bidirectional diffusion of nutrients, oxygen, waste that promote survival in the target tissues. Here, we describe simple scalable emulsification method encapsulate animal chitosan microbeads using thermosensitive gel formulations without any chemical modification cross-linker. The process consists water-in-oil emulsion where aqueous phase droplets...
Diabetes is a devastating disease that ultimately caused by the malfunction or loss of insulin-producing pancreatic beta-cells. Drugs capable inducing development new beta-cells improving function survival existing could conceivably cure this disease. We report novel high-throughput screening platform exploits multi-parameter high-content analysis to determine effect compounds on beta-cell survival, as well promoter activity two key genes, insulin and pdx1. Dispersed human islets MIN6 were...
Ethyl lactate is injected into a dielectric barrier discharge (DBD) to build up degradable plasma polymer (PP) be used as drug delivery system. Plasma power, deposition time, and type of carrier gas (Ar, N2) are correlated the coating in vitro degradation rate. PPs characterized by AFM, SEM, IR spectroscopy, XPS, SEC, while surface profilometry monitor kinetics. deposited under N2 mainly composed hydrophilic functionalities, which explain their fast upon exposure an aqueous environment. In...
Today's Biochemical Engineer may contribute to advances in a wide range of technical areas. The recent and Molecular Engineering XXI conference focused on "The Next Generation Engineering: role emerging technologies tomorrow's products processes". On the basis topical discussions at this conference, perspective synthesizes one vision where investment research areas is needed for biotechnology continue contributing some world's grand challenges.
Islet encapsulation devices serve to deliver pancreatic beta cells type 1 diabetic patients without the need for chronic immunosuppression. However, clinical translation is hampered by mass transport limitations causing graft hypoxia. This exacerbated in relying only on passive diffusion oxygenation. Here, we describe application of a cylindrical vitro perfusion system study oxygen effects islet-like clusters immobilized alginate hydrogel. Mouse insulinoma 6 were generated using microwell...
The proliferation of pancreatic duct-like CK19+ cells has implications for multiple disease states including cancer and diabetes mellitus. in vitro study this important cell type been hampered by their limited expansion compared to fibroblast-like vimentin+ that overgrow primary cultures. We aimed develop a screening platform duct mitogens after depletion the population. CD90 surface marker was used remove from islet-depleted human pancreas cultures magnetic-activated sorting. Cell sorting...
Abstract Intimal hyperplasia and thrombosis are responsible for the poor patency rates of small‐diameter vascular grafts. These complications could be avoided by a rapid strong adhesion endothelial cells to prosthetic surfaces, which typically consist expanded polytetrafluoroethylene (PTFE) vessels. We have previously described two peptide micropatterning strategies that increase endothelialization PTFE. The micropatterns were generated either inkjet printing 300 μm squares or spraying 10.1...