A5080753695- Advanced MRI Techniques and Applications
- Electron Spin Resonance Studies
- Osteoarthritis Treatment and Mechanisms
- Advanced NMR Techniques and Applications
- Cancer, Hypoxia, and Metabolism
- NMR spectroscopy and applications
- Pancreatic function and diabetes
- Solid-state spectroscopy and crystallography
- Photoacoustic and Ultrasonic Imaging
- Tissue Engineering and Regenerative Medicine
- Lanthanide and Transition Metal Complexes
- Atomic and Subatomic Physics Research
- Nanoplatforms for cancer theranostics
- MRI in cancer diagnosis
- Mesenchymal stem cell research
- Electrospun Nanofibers in Biomedical Applications
- Total Knee Arthroplasty Outcomes
- Cardiovascular Function and Risk Factors
- Muon and positron interactions and applications
- Bone Tissue Engineering Materials
- Microfluidic and Capillary Electrophoresis Applications
- Proteoglycans and glycosaminoglycans research
- Knee injuries and reconstruction techniques
- Collagen: Extraction and Characterization
- 3D Printing in Biomedical Research
HEM Technologies (United States)
2021-2025
University of Illinois Chicago
2008-2017
Bioengineering Center
2001-2014
University of Duisburg-Essen
2006
Rani Durgavati University
2000-2006
Weizmann Institute of Science
2003-2005
State University of New York
2005
Abstract Inadequate oxygenation is a major challenge in cell encapsulation, therapy which holds potential to treat many diseases including type I diabetes. In such systems, cellular oxygen (O 2 ) delivery limited slow passive diffusion from transplantation sites through the poorly O -soluble encapsulating matrix, usually hydrogel. This constrains maximum permitted distance between encapsulated cells and host site within few hundred micrometers ensure function. Inspired by natural gas-phase...
A cell encapsulation system generates oxygen from carbon dioxide for self-oxygenation.
The use of a low-power two-pulse phase modulation (TPPM) sequence is proposed for efficient 1H radio frequency (rf) decoupling in high-resolution 13C solid-state NMR (SSNMR) under fast MAS conditions. Decoupling efficiency different sequences such as continuous-wave (cw), TPPM, XiX, and π-pulse (PIPS) train has been investigated at spinning speed 40 kHz CPMAS spectra uniformly 13C- 15N-labeled L-alanine. It was found that the TPPM sequence, which originally designed high-power decoupling,...
Engineering biomaterials mimicking the biofunctionality of extracellular matrix (ECM) is important in instructing and eliciting cell response. The native ECM highly dynamic has been shown to support cellular attachment, migration, differentiation. advantage synthesizing an ECM-based biomaterial that it mimics environment. However, tissue-specific composition patterned arrangement. In this study, we have employed biomimetic strategies develop a novel collagen/chitosan template embedded with...
Abstract The use of oxygen by cells is an essential aspect cell metabolism and a reliable indicator viable functional cells. Here, we report partial pressure (pO 2 ) mapping live as metabolically active For pO imaging, utilized trityl OX071-based pulse electron paramagnetic resonance imaging (EPROI), in combination with 25 mT EPROI instrument, JIVA-25™, that provides 3D maps high spatial, temporal, resolution. To perform environment-controlled apparatus, developed novel multi-well-plate...
Abstract Type 1 diabetes (T1D) is an autoimmune disease that leads to the loss of insulin-producing beta cells. Bioartificial pancreas (BAP) or cell replacement strategies have shown promise in curing T1D and providing long-term insulin independence. Hypoxia (low oxygen concentration) may occur BAP devices due consumption at early stages after implantation damages cells, addition imposing limitations device dimensions when translating promising results from rodents humans. Finding ways...
Abstract Purpose The determination of blood–brain barrier (BBB) integrity and partial pressure oxygen (pO 2 ) in the brain is substantial interest several neurological applications. This study aimed to assess feasibility using trityl OX071‐based pulse electron paramagnetic resonance imaging (pEPRI) provide a quantitative estimate BBB pO maps mouse brains as function neuroinflammatory disease progression. Methods Five Connexin‐32 (Cx32)–knockout (KO) mice were injected with lipopolysaccharide...
Abstract Purpose Solid crystalline spin probes, such as lithium phthalocyanine (LiPc) and octa‐n‐butoxynaphthalocyanine (LiNc‐BuO), allow repeated oxygen measurement using electron paramagnetic resonance (EPR). Due to their short relaxation times, use for pulse EPR imaging is limited. In this study, we developed tested a new class of solid composite probes that modified the rates R 1 2 LiPc or LiNc‐BuO which allowed pO measurements in full dynamic (0–760 torr) range. Methods The were by...
Abstract Purpose Trityl OXO71‐based pulse electron paramagnetic resonance imaging (EPRI) is an excellent technique to obtain partial pressure of oxygen (pO 2 ) maps in tissues. In this study, we used deep learning techniques denoise 3D EPR amplitude and pO maps. Methods All experiments were performed using a 25 mT imager, JIVA‐25®. The MONAI implementation four neural networks (autoencoder, Attention UNet, UNETR, UNet) was tested, the best model (UNet) then enhanced with joint bilateral...
Abstract Background: Interstitial photodynamic therapy (I-PDT) is a promising treatment for patients with large locally advanced or deeply seated tumors. In I-PDT, light administered to activate photosensitive drug (photosensitizer), which, in the presence of oxygen, results production reactive oxygen species. previous preclinical studies, we demonstrated that dose rate (irradiance, mW/cm2) main predictor tumor response I-PDT. The objective this investigational study was perform electron...
Clinical attempts to find benefit from specifically targeting and boosting resistant hypoxic tumor subvolumes have been promising but inconclusive. While a first preclinical murine type showed significant improved control with boosts, more thorough investigation of efficacy defined by electron paramagnetic resonance oxygen imaging (EPROI) is necessary. The present study confirms results in three different types using clonogenic assay explores potential confounding experimental...
The purpose of this study was to assess the natural partial oxygen pressure (pO2) subcutaneous (SC) and intraperitoneal (IP) sites in mice determine their relative suitability as for placement implants. pO2 measurements were performed using imaging solid probes lithium phthalocyanine (LiPc) sensitive material. LiPc is a water-insoluble crystalline probe whose spin-lattice spin-spin relaxation rates (R1 R2) are local concentration. To facilitate direct vivo imaging, we prepared containing...
A copolymer composed of poly(2-hydroxyethyl methacrylate) (PHEMA) and poly(ethylene glycol) diacrylate (PEGDA) (PHEMA-PEGDA) is structurally versatile. Its structure can be adjusted using the following porogens: water, sucrose, benzyl alcohol. Using phase separation technique, a variety surface architectures pore morphologies were developed by adjusting porogen volume type. The water sucrose porogens effective in creating porous cytocompatible PHEMA-PEGDA scaffolds. When coated with...
We studied the tissue growth dynamics of tissue‐engineered cartilage at an early stage after cell seeding for four weeks using sodium triple‐quantum coherence NMR spectroscopy. The following tissue‐engineering constructs were studied: 1) bovine chondrocytes cultured in alginate beads; 2) as pellets (scaffold‐free chondrocyte pellets); and 3) human marrow stromal cells (HMSCs) seeded collagen/chitosan based biomimetic scaffolds. found that spectroscopy could differentiate between different...
Oxygen is the single most important molecule for sustaining life and, therefore, an variable in tissue engineering and regenerative medicine. It has been shown that change oxygen concentration artificial or tissue-engineered graft affects cell survival, differentiation, growth profound ways. However, at present, there are no reliable methods to map partial pressure (pO2) growing tissues. Here, we adapt test suitability of electron paramagnetic resonance imaging (EPROI) assessing oxygenation...