A5028571521- Immune Cell Function and Interaction
- Biomedical Ethics and Regulation
- T-cell and B-cell Immunology
- Immunodeficiency and Autoimmune Disorders
- Immunotherapy and Immune Responses
- Research on Leishmaniasis Studies
- Immune Response and Inflammation
- CAR-T cell therapy research
- Food Allergy and Anaphylaxis Research
- Pluripotent Stem Cells Research
- Allergic Rhinitis and Sensitization
- Trypanosoma species research and implications
- RNA Interference and Gene Delivery
- Asthma and respiratory diseases
- RNA and protein synthesis mechanisms
- Aquaculture disease management and microbiota
- Sperm and Testicular Function
- RNA Research and Splicing
- Monoclonal and Polyclonal Antibodies Research
- CRISPR and Genetic Engineering
- T-cell and Retrovirus Studies
- Toxin Mechanisms and Immunotoxins
- Science, Research, and Medicine
- Vector-Borne Animal Diseases
- Biosimilars and Bioanalytical Methods
Agencia Española de Medicamentos y Productos Sanitarios
2006-2024
Jenner Institute
1997-2001
Universidad Complutense de Madrid
1992-1996
Hospital Universitario 12 De Octubre
1994-1996
Research Institute Hospital 12 de Octubre
1991-1993
PRIMARY immunodeficiency diseases are a heterogeneous group of disorders resulting from intrinsic defects the immune system.1 They frequently associated with repeated bacterial, fungal, or v...
There is no gold standard for diagnosing leishmaniases. Our aim was to assess the operative validity of tests used in detecting Leishmania infection using samples from experimental infections, a reliable equivalent classic definition standard. Without statistical differences, highest sensitivity achieved by protein A (ProtA), immunoglobulin (Ig)G2, indirect fluorescenece antibody test (IFAT), lymphocyte proliferation assay, quantitative real-time polymerase chain reaction bone marrow...
A T cell line termed DIL2 has been derived from an infant with a polyclonal receptor (TCR)/CD3 surface expression defect. Indirect immunofluorescence showed that the of certain TCR/CD3 epitopes (like those detected by WT31 and BMA031 monoclonals) was strongly reduced (around five-fold) on DIL2, whereas other SP34 Leu4) were only around two-fold lower than in normal lines. Specific immunoprecipitates surface-radioiodinated cells contained TCR-alpha, TCR-beta, CD3-delta, CD3-epsilon TCR-zeta...
Great advances have been made in the knowledge of development and regulatory approval medicinal product containing genetically modified cells. Although a guideline has available EU since 2012, current updated version provides useful guide to developers professionals involved process these medicines. This article presents main issues communicated that guidance, regulators' insights commentary from academic developers' point view.
The leukocyte common antigen (CD45) is alternatively spliced, generating various isoforms expressed on hemopoietic cells. splicing pattern of CD45 in T cells altered some individuals who show abnormal expression high molecular weight containing exon A. variant was shown to be associated with heterozygosity for a silent point mutation within This C G transition located 77 nucleotides downstream the splice acceptor junction A (198 bp total length). Here we report that this cause splicing. To...
A diploid expression of class I and II human leukocyte antigens (HLA) has been found in purified spermatozoa by using double fluorescence labeling cytofluorometry relevant monoclonal antibodies; this confirmed for the first time analysis specific HLA mRNA metabolic 35S followed immunoprecipitation, which demonstrates an active ongoing translation proteins germinal cells. Long-living coming from cells may be translated to molecules spermatozoa. This is controlled (or at least inversely...
Abstract The selection of T lymphocytes in the thymus and their activation upon encounter with foreign antigens periphery require aggregation signals Tcell receptor (TcR)/CD3 complex several surface molecules termed coreceptors (notably CD4 or CD8 CD45). spatial arrangement interactions different resulting multimolecular recognition structure are mostly unknown. Here we report, from studies on a healthy human CD3γ deficiency, that lack component TcR/CD3 is associated long‐term severe defect...
SUMMARY Cytomegalovirus(CMV) infection is reported to cause transient immunosuppression in man. In this Study we have analysed ihe effect of CMV on T lymphocyte function 29 children diagnosed for acute infection. Peripheral blood mononuclear cells (PBMC) obtained from the palients showed a significant specific impairment their proliferative response enterotoxins A and C1. coneanavalin anti-CD3 MoAb OKT3. The impaired responses were corrected with exogenous IL-2. conlrast. slimulation using...