A5037607065- Bacterial biofilms and quorum sensing
- Advanced Proteomics Techniques and Applications
- Metabolomics and Mass Spectrometry Studies
- Cystic Fibrosis Research Advances
- Plant nutrient uptake and metabolism
- Plant Stress Responses and Tolerance
- Plant responses to water stress
- Mass Spectrometry Techniques and Applications
- Ruminant Nutrition and Digestive Physiology
- Chromium effects and bioremediation
- Alzheimer's disease research and treatments
- GABA and Rice Research
- Plant Reproductive Biology
- Biotin and Related Studies
- Advanced Biosensing Techniques and Applications
- Trace Elements in Health
- Retinal Development and Disorders
- Fungal and yeast genetics research
- Soybean genetics and cultivation
- Research in Cotton Cultivation
- Bacterial Genetics and Biotechnology
- Heavy metals in environment
- Biofuel production and bioconversion
- Antibiotic Resistance in Bacteria
- Muscle metabolism and nutrition
Macquarie University
2016-2025
Australian Genome Research Facility
2017-2025
Universidade de São Paulo
2023
Mahatma Gandhi Institute of Medical Sciences
2022
Narayana Health
2013
Thrombosis Research Institute
2013
Indian Institute of Technology Bombay
2011
Abstract Clustering Epilepsy (CE) is a neurological disorder caused by pathogenic variants of the Protocadherin 19 (PCDH19) gene. PCDH19 encodes protein involved in cell adhesion and Estrogen Receptor α mediated-gene regulation. To gain further insights into molecular role brain, we investigated interactome developing mouse hippocampus cortex. Combined with meta-analysis all reported interacting proteins, our results show that interacts proteins actin, microtubule, gene We report CAPZA1,...
Thirty-six Merino wethers (10-month-old) were fed ad libitum for 30 days two diets;1) low metabolizable energy diet (LME; 30% lucerne: 70% cereal chaff) and 2) high ME (HME; 40% rolled barley grain: 50% 10% chaff). Effects of on dry matter intake (DMI), (MEI), liveweight (LWT), average daily gain (ADG), carcass lean or fat gain, liver empty rumen weight plasma metabolites analysed. A membrane enriched protein fraction epithelium isolated enzymatically from whole depth wall was quantified...
Pseudomonas aeruginosa is a ubiquitous Gram-negative pathogen known to inhabit hypoxic mucus plugs of cystic fibrosis (CF) patient lungs. Despite the high prevalence and related mortality, protein machinery enabling bacterium adapt low oxygen environment remains be fully elucidated. We investigated this by performing both SWATH mass spectrometry data-dependent SPS-MS3 TMT-labeled peptides profile proteomes two P. CF isolates, PASS2 PASS3, laboratory reference strain, PAO1, grown under stress...
The opportunistic pathogen Pseudomonas aeruginosa is among the main colonizers of lungs cystic fibrosis (CF) patients. We have isolated and sequenced several P. isolates from sputum CF patients compared them with each other model strain PAO1. Phenotypic analysis showed significant variability in colonization virulence-related traits suggesting different strategies for adaptation to lung. Genomic indicated these strains shared a large set core genes standard laboratory PAO1, identified...
Rice crops are often subject to multiple abiotic stresses simultaneously in both natural and cultivated environments, resulting yield reductions beyond those expected from single stress. We report physiological changes after a 4 day exposure combined drought, salt extreme temperature treatments, following 2 salinity pre-treatment two rice genotypes-Nipponbare (a paddy rice) IAC1131 (an upland landrace). Stomata closed days of stresses, causing intercellular CO2 concentrations assimilation...
Abstract Background Trichoderma reesei is an organism extensively used in the bioethanol industry, owing to its capability produce enzymes capable of breaking down holocellulose into simple sugars. The uptake carbohydrates generated from cellulose breakdown crucial induce signaling cascade that triggers cellulase production. However, sugar transporters involved this process T. remain poorly identified and characterized. Results To address gap, study temporal membrane proteomics analysis...
Pseudomonas aeruginosa is a Gram-negative, nosocomial, highly adaptable opportunistic pathogen especially prevalent in immuno-compromised cystic fibrosis (CF) patients. The bacterial cell surface proteins are important contributors to virulence, yet the membrane subproteomes of phenotypically diverse P. strains poorly characterized. We carried out mass spectrometry (MS)-based proteome analysis three novel isolated from sputum CF patients and compared protein expression widely used laboratory...
Amyloid β (Aβ) accumulation and its aggregation is characteristic molecular feature of the development Alzheimer's disease (AD). More recently, Aβ has been suggested to be associated with retinal pathology AD, glaucoma drusen deposits in age related macular degeneration (AMD). In this study, we investigated proteins biochemical networks that are affected by amyloid 661W photoreceptor cells culture. Time dose dependent effects on were determined utilising tandem mass tag (TMT) labelling-based...
Rice is a critically important food source but yields worldwide are vulnerable to periods of drought. We exposed eight genotypes upland and lowland rice (Oryza sativa L. ssp. japonica indica) drought stress at the late vegetative stage, harvested leaves for label-free shotgun proteomics. Gene ontology analysis was used identify common drought-responsive proteins in tissues, leaf that unique individual genotypes, suggesting diversity metabolic responses Eight were found be induced response...
The E. gracilis Zm-strain lacking chloroplasts, characterized in this study, was compared with the earlier assessed wild type Z-strain to explore role of chloroplasts heavy metal accumulation and tolerance. Comparison minimum inhibitory concentration (MIC) values indicated that both strains tolerated similar concentrations mercury (Hg) lead (Pb), but cadmium (Cd) tolerance twice Zm-strain. ability accumulate Hg higher Z-strain, indicating existence a transportation mechanism not depending on...
The opportunistic pathogen, Pseudomonas aeruginosa is well known for its environmental and metabolic versatility, yet many of the functions gene-products remain to be fully elucidated. This study's objective was illuminate potential under-described during medically relevant copper-stress condition. We used data-independent acquisition mass spectrometry quantitate protein expression changes associated with copper stress in P. PAO1. Approximately 2000 non-redundant proteins were quantified, 78...
Drought often compromises yield in non-irrigated crops such as rainfed rice, imperiling the communities that depend upon it a primary food source. In this study, two cultivated species (Oryza sativa cv. Nipponbare and Oryza glaberrima CG14) an endemic, perennial Australian wild australiensis) were grown soil at 40% field capacity for 7 d (drought). The hypothesis was natural tolerance of O. australiensis to erratic water supply would be reflected unique proteomic profile. Leaves from...
Fluoroquinolones are one of the most prescribed broad-spectrum antibiotics. However, their effectiveness is being compromised by high rates resistance in clinically important organisms, including Acinetobacter baumannii We sought to investigate transcriptomic and proteomic responses clinical A. strain AB5075-UW upon exposure subinhibitory concentrations ciprofloxacin. Our transcriptomics proteomics analyses found that highly expressed genes proteins were components intact prophage phiOXA....
Abstract This experiment was carried out to provide a comprehensive insight into the protein activities involved in dormancy establishment seeds of common cocklebur ( Xanthium strumarium ), an annual plant with two dimorphic contained one casing known as burr. These consist smaller dormant seed and larger non‐dormant seed. The proteome profile compared between developing at five consecutive stages including three, 10, 20, 30, 45 days after burr emergence (stages 1 5). We identified 6524...
Credible detection and quantification of low abundance proteins from human blood plasma is a major challenge in precision medicine biomarker discovery when using mass spectrometry (MS). In this proof-of-concept study, we employed mixture selected recombinant DDA libraries to subsequently identify (not quantify) cancer-associated SWATH/DIA. The exemplar protein spectral library (rPSL) was derived tryptic digestion 36 that had been previously implicated as possible cancer biomarkers both our...
Extracellular deposits of the amyloid-beta peptide (Aβ) are known as main pathological hallmark Alzheimer's disease. In disease, neurons injured and die throughout brain, a process in which Aβ neurotoxicity is considered to play an important role. However, molecular mechanisms underlying toxicity that lead neurodegeneration not clearly established. Here we have elucidated pathways networks impacted by using SH-SY5Y human neuroblastoma cells model. These were treated with Aβ1–42 peptides...
Heat stress specifically affects fertility by impairing pollen viability but cotton wild relatives successfully reproduce in hot savannas where they evolved. An Australian arid-zone (Gossypium robinsonii) was exposed to heat events during development then mature subjected deep proteomic analysis using 57 023 predicted genes from a genomic database we assembled for the same species. Three stages of development, including tetrads (TEs), uninucleate microspores (UNs) and binucleate (BNs) were...
Background: Multi-marker approaches for risk prediction in coronary artery disease (CAD) have been inconsistent due to biased selection of specific know biomarkers. We assessed the global proteome CAD-affected and unaffected subjects, developed a pathway network model elucidating mechanism CAD. Materials Methods: A total 252 samples (112 without family history 140 true controls) were analyzed by Surface-Enhanced Laser Desorption/Ionization Time Flight Mass Spectrometry (SELDI-TOF-MS) using...