A5037593442- Fungal and yeast genetics research
- Heavy metals in environment
- Moringa oleifera research and applications
- Endoplasmic Reticulum Stress and Disease
- Biofuel production and bioconversion
- Microbial Metabolic Engineering and Bioproduction
- Fermentation and Sensory Analysis
- Chromium effects and bioremediation
- Plant Stress Responses and Tolerance
- Plant nutrient uptake and metabolism
- Protein Kinase Regulation and GTPase Signaling
- Plant pathogens and resistance mechanisms
- Heavy Metal Exposure and Toxicity
- Heavy Metals in Plants
- Photosynthetic Processes and Mechanisms
- ATP Synthase and ATPases Research
- Aluminum toxicity and tolerance in plants and animals
- RNA Research and Splicing
- Pesticide Exposure and Toxicity
- Metal Extraction and Bioleaching
- Andrographolide Research and Applications
- Plant Gene Expression Analysis
- Pesticide and Herbicide Environmental Studies
- Genetics, Aging, and Longevity in Model Organisms
- Heat shock proteins research
Mahidol University
2012-2024
Ministry of Education
2015-2022
Thai Health Promotion Foundation
2020
Osaka University
2003-2005
In Saccharomyces cerevisiae, the phosphate signal transduction PHO pathway is involved in regulating several phosphate-responsive genes such as PHO5, which encodes repressible acid phosphatase. this pathway, a cyclin-dependent kinase inhibitor (Pho81p) regulates activity of cyclin-cyclin-dependent complex Pho80p-Pho85p, phosphorylates transcription factor Pho4p response to intracellular levels. However, how cells sense availability and transduce Pho81p remains unknown. To identify additional...
High-temperature ethanol fermentation has several benefits including a reduction in cooling cost, minimizing risk of bacterial contamination, and enabling simultaneous saccharification fermentation. To achieve the efficient at high temperature, yeast strain that tolerates to not only temperature but also other stresses present during fermentation, e.g., ethanol, osmotic, oxidative stresses, is indispensable. The C3253, C3751, C4377 Saccharomyces cerevisiae strains, which have been previously...
During fermentation, a high ethanol concentration is major stress that influences the vitality and viability of yeast cells, which in turn leads to termination fermentation process. In this study, we show BCK1 SLT2 genes encoding mitogen-activated protein kinase (MAPKKK) (MAPK) cell wall integrity (CWI) pathway, respectively, are essential for tolerance, suggesting CWI pathway involved response ethanol-induced stress. Upon exposure, induces expression specific wall-remodeling genes,...
During fermentation, increased ethanol concentration is a major stress for yeast cells. Vacuolar H(+)-ATPase (V-ATPase), which plays an important role in the maintenance of intracellular pH homeostasis through vacuolar acidification, has been shown to be required tolerance straight-chain alcohols, including ethanol. Since known increase membrane permeability protons, then promotes it possible that V-ATPase recovery from alcohol-induced acidification. In this study, we show effects alcohols...
During fermentation, yeast cells encounter a number of stresses, including hyperosmolarity, high ethanol concentration, and temperature. Previous deletome analysis in the Saccharomyces cerevisiae has revealed that SOD1 gene encoding cytosolic Cu/Zn-superoxide dismutase (SOD), major antioxidant enzyme, was required for tolerances to not only oxidative stress but also other stresses present during fermentation such as osmotic, ethanol, heat stresses. It is therefore possible these...
The Greatwall-family protein kinase Rim15 is associated with the nutrient starvation response, whereas its role in oxidative stress responses remains unclear. Here, acetic acid and peroxide were used as two elicitors. antioxidant indicator assay under revealed impaired growth rim15Δ related to regulation of systems. Comparative transcriptome analysis that differentially expressed genes (DEGs) are predicted be mostly regulated by stress-responsive transcriptional factor Yap1. Among DEGs,...
Contamination of soil and water with heavy metals metalloids is a serious environmental problem. Cadmium arsenic are major contaminants that pose threat to human health. Although toxicities cadmium living organisms have been extensively studied, the molecular mechanisms cellular responses remain poorly understood. In this study, we demonstrate cell wall integrity (CWI) pathway involved in coping stresses induced by arsenate through its role regulation modification. Interestingly, Rlm1p SBF...
In Saccharomyces cerevisiae, vacuolar H+ -ATPase (V-ATPase) involved in the regulation of intracellular pH homeostasis has been shown to be important for tolerances cadmium, cobalt and nickel. However, molecular mechanism underlying protective role V-ATPase against these metals remains unclear. this study, we show that nickel disturbed balance by triggering cytosolic acidification alkalinization, likely via their membrane permeabilizing effects. Since plays a crucial pumping excessive...
Alachlor, a widely used chloroacetanilide herbicide for controlling annual grasses in crops, has been reported to rapidly trigger protein denaturation and aggregation the eukaryotic model organism Saccharomyces cerevisiae. Therefore, this study aimed uncover cellular mechanisms involved preventing alachlor-induced proteotoxicity. The findings reveal that ubiquitin-proteasome system (UPS) plays crucial role eliminating alachlor-denatured proteins by tagging them with polyubiquitin subsequent...