A5082482679- Fungal and yeast genetics research
- Microbial Metabolic Engineering and Bioproduction
- Biofuel production and bioconversion
- CRISPR and Genetic Engineering
- Fermentation and Sensory Analysis
- RNA Research and Splicing
- RNA and protein synthesis mechanisms
- Genomics and Chromatin Dynamics
- Plant nutrient uptake and metabolism
- Chromosomal and Genetic Variations
- Plant Reproductive Biology
- Yeasts and Rust Fungi Studies
- Plant tissue culture and regeneration
- Endoplasmic Reticulum Stress and Disease
- Polyamine Metabolism and Applications
- Viral Infectious Diseases and Gene Expression in Insects
- Bacterial Genetics and Biotechnology
- Plant Disease Resistance and Genetics
- Transgenic Plants and Applications
- Lipid metabolism and biosynthesis
- Fungal Biology and Applications
- Protein Kinase Regulation and GTPase Signaling
- Plant biochemistry and biosynthesis
- Plant Gene Expression Analysis
- DNA Repair Mechanisms
Sojo University
2016-2023
Osaka University
2008-2020
Universitas Gadjah Mada
2017
Mahidol University
2012
Eunice Kennedy Shriver National Institute of Child Health and Human Development
1986-1991
National Institutes of Health
1987
The PHO84 gene specifies Pi-transport in Saccharomyces cerevisiae. A DNA fragment bearing the was cloned by its ability to complement constitutive synthesis of repressible acid phosphatase pho84 mutant cells. Its nucleotide sequence predicted a protein 596 amino acids with homologous that superfamily sugar transporters. Hydropathy analysis suggested secondary structure consists two blocks six transmembrane domains separated 74 residues. PH084 restored Pi transport activity transcription...
The PHO84 gene specifies Pi-transport in Saccharomyces cerevisiae. A DNA fragment bearing the was cloned by its ability to complement constitutive synthesis of repressible acid phosphatase pho84 mutant cells. Its nucleotide sequence predicted a protein 596 amino acids with homologous that superfamily sugar transporters. Hydropathy analysis suggested secondary structure consists two blocks six transmembrane domains separated 74 residues. PH084 restored Pi transport activity transcription...
A higher plant homologue to the high-affinity phosphate transporter gene of yeast ( Saccharomyces cerevisiae ) PHO84 was isolated from Arabidopsis thaliana . Expression PHT1 at high levels in tobacco-cultured cells increased rate uptake. The uptake activity attributable transgene inhibited by protonophores, suggesting an H + cotransport mechanism uptake, and had a K m 3.1 μM which is within limits characteristic transport mechanisms. These results indicate that encodes transporter....
The GCD2 protein is a translational repressor of GCN4, the transcriptional activator multiple amino acid biosynthetic genes in Saccharomyces cerevisiae. We present evidence that has general function initiation synthesis addition to its gene-specific role control GCN4 expression. Two temperature-sensitive lethal gcd2 mutations result sensitivity inhibitors at permissive temperature, and gcd2-503 mutation leads reduced incorporation labeled leucine into total following shift restrictive...
The term 'sake yeast' is generally used to indicate the Saccharomyces cerevisiae strains that possess characteristics distinct from others including laboratory strain S288C and are well suited for sake brewery. Here, we report draft whole-genome shotgun sequence of a commonly diploid yeast strain, Kyokai no. 7 (K7). assembled K7 was nearly identical S288C, except several subtelomeric polymorphisms two large inversions in K7. A survey heterozygous bases between homologous chromosomes revealed...
In Saccharomyces cerevisiae, the phosphate signal transduction PHO pathway is involved in regulating several phosphate-responsive genes such as PHO5, which encodes repressible acid phosphatase. this pathway, a cyclin-dependent kinase inhibitor (Pho81p) regulates activity of cyclin-cyclin-dependent complex Pho80p-Pho85p, phosphorylates transcription factor Pho4p response to intracellular levels. However, how cells sense availability and transduce Pho81p remains unknown. To identify additional...
ABSTRACT There are four types of life cycles in Saccharomyces cerevisiae and its related species. A perfect homothallic cycle (the Ho type) is observed the classic D strain. Two other show semi-homothallism; one them shows a 2-homothallic diploid:2α heterothallic haploid segregation Hp another, 2-homothallic:2a Hq type). In segregants from these Ho, Hp, diploids, each segregant same pattern as parental diploid. The fourth type has showing 2a:2α diploids produced by fusion two cells opposite...
High-temperature ethanol fermentation has several benefits including a reduction in cooling cost, minimizing risk of bacterial contamination, and enabling simultaneous saccharification fermentation. To achieve the efficient at high temperature, yeast strain that tolerates to not only temperature but also other stresses present during fermentation, e.g., ethanol, osmotic, oxidative stresses, is indispensable. The C3253, C3751, C4377 Saccharomyces cerevisiae strains, which have been previously...
ABSTRACTTranscriptional coactivators play a crucial role in gene expression by communicating between regulatory factors and the basal transcription machinery. The coactivator multiprotein bridging factor 1 (MBF1) was originally identified as molecule that connects Drosophila nuclear receptor FTZ-F1 TATA-binding protein (TBP). MBF1 sequence is highly conserved across species fromSaccharomyces cerevisiae to human. Here we provide evidence acquired vitro vivo yeast mediates GCN4-dependent...
Thirty-two protein phosphatase (PPase) genes were identified in the genome nucleotide sequence of Saccharomyces cerevisiae. We constructed S. cerevisiae disruptants for each PPase and examined their growth under various conditions. The six putative genes, i.e. YBR125c, YCR079w, YIL113w, YJR110w, YNR022c YOR090c, created first time this study. glc7, sit4 cdc14 lethal our strain background. remaining 29 gene viable at 30°C 37°C, but only one disruptant, yvh1, showed intrinsic cold-sensitive...
GCN4 encodes a positive regulator of multiple unlinked genes encoding amino acid biosynthetic enzymes in Saccharomyces cerevisiae. Expression is coupled to availability by control mechanism involving GCD1 as negative effector and GCN1, GCN2, GCN3 effectors expression. We used reversion gcn2 gcn3 double mutation isolate new alleles mutations four additional GCD which we designate GCD10, GCD11, GCD12, GCD13. All the lead constitutive derepression HIS4 transcription absence GCN2+ GCN3+ alleles....
We have found an open reading frame which is 1.1 kb upstream of PHO84 (which encodes a Pi transporter) and transcribed from the opposite strand. In Saccharomyces cerevisiae, this gene distal to TUB3 locus on left arm chromosome XIII named GTR1. GTR1 protein consisting 310 amino acid residues containing, in its N-terminal region, characteristic tripartite consensus elements for binding GTP conserved GTP-binding proteins, except histidine place widely aspargine residue element III. Disruption...
GCN4 encodes a transcriptional activator in Saccharomyces cerevisiae that is regulated at the translational level. We show an approximately 240-nucleotide segment from mRNA leader containing four AUG codons sufficient to confer control typical of upon GAL1-lacZ fusion transcript. Regulation hybrid transcript dependent multiple positive (GCN) and negative (GCD) trans-acting factors shown regulate expression post-transcriptionally. This result limits target sequences for these small internal...
Abstract Background Ethanol production from paper sludge (PS) by simultaneous saccharification and fermentation (SSF) is considered to be the most appropriate way process PS, as it contains negligible lignin. In this study, SSF was conducted using a cellulase produced PS hypercellulase producer, Acremonium cellulolyticus C-1 for saccharification, thermotolerant ethanol producer Saccharomyces cerevisiae TJ14 production. Using of origin minimizes biofuel costs, because culture broth containing...
We have cloned a DNA fragment complementing the aar1 mutation defective in a1-alpha 2 repression of alpha 1 cistron and haploid-specific genes Saccharomyces cerevisiae. Nucleotide sequence mapping data indicated that AAR1 gene is identical with TUP1, which allelic to SFL2, FLK1, CYC9, UMR7, AMM1, AER2 genes, whose mutations are known confer variety phenotypes, such as thymidine uptake, flocculation, insensitivity glucose repression, defect UV-induced mutagenesis, ARS plasmid maintenance. The...