A5011662830- Fungal and yeast genetics research
- Biofuel production and bioconversion
- Microbial Metabolic Engineering and Bioproduction
- RNA and protein synthesis mechanisms
- Fermentation and Sensory Analysis
- Enzyme Production and Characterization
- CRISPR and Genetic Engineering
- Yeasts and Rust Fungi Studies
- Protist diversity and phylogeny
- Autophagy in Disease and Therapy
- Transgenic Plants and Applications
- Bacterial Genetics and Biotechnology
- RNA Research and Splicing
- Enzyme Structure and Function
- Endoplasmic Reticulum Stress and Disease
- Ubiquitin and proteasome pathways
- Plant-Microbe Interactions and Immunity
- Microbial Metabolism and Applications
- Molecular Biology Techniques and Applications
- RNA modifications and cancer
- DNA Repair Mechanisms
- Viral Infectious Diseases and Gene Expression in Insects
- Lichen and fungal ecology
- Microbial Natural Products and Biosynthesis
- Photosynthetic Processes and Mechanisms
Yamaguchi University
2014-2024
Hiroshima University
1985-1997
The University of Tokyo
1996
Tokyo University of Science
1996
University of Vermont
1996
Hiroshima University of Economics
1994
We demonstrate herein the ability of Kluyveromyces marxianus to be an efficient ethanol producer and host for expressing heterologous proteins as alternative Saccharomyces cerevisiae. Growth production by strains K. S. cerevisiae were compared under same conditions. DMKU3-1042 was found most suitable strain high-temperature growth at 45 degrees C. This strain, but not cerevisiae, utilized cellobiose, xylose, xylitol, arabinose, glycerol, lactose. To develop a derivative genetic engineering,...
The term 'sake yeast' is generally used to indicate the Saccharomyces cerevisiae strains that possess characteristics distinct from others including laboratory strain S288C and are well suited for sake brewery. Here, we report draft whole-genome shotgun sequence of a commonly diploid yeast strain, Kyokai no. 7 (K7). assembled K7 was nearly identical S288C, except several subtelomeric polymorphisms two large inversions in K7. A survey heterozygous bases between homologous chromosomes revealed...
Autophagy is an intracellular degradation system by which cytoplasmic materials are enclosed autophagosome and delivered to a lysosome/vacuole. Atg18 plays critical role in formation as complex with Atg2 phosphatidylinositol 3-phosphate (PtdIns(3)P). However, little known about the structure of its recognition mode or PtdIns(3)P. Here, we report crystal Kluyveromyces marxianus Hsv2, paralog, at 2.6 Å resolution. The reveals seven-bladed β-propeller without circular permutation. Mutational...
Abstract Repeated gene manipulations can be performed in yeast by excision of an introduced marker. Cassette modules containing a marker flanked two direct repeat sequences hisG or loxP have often been used for recycling, but these leave one copy the repeats chromosome after excision. Genomic copies cause increased mistargeting constructs same unexpected chromosomal rearrangements via intra‐ interchromosomal recombinations. Here, we describe novel recycling procedure that leaves no scar...
Abstract Kluyveromyces marxianus DMKU3‐1042 is a thermotolerant yeast strain suitable for high‐temperature ethanol fermentation and genetic engineering with linear DNA. We have developed highly efficient random gene integration method frequency that exceeds 2.5 × 10 6 transformants/µg DNA, figure comparable to what observed autonomously replicating plasmid transformation in Saccharomyces cerevisiae . To establish the mechanism of DMKU3‐1042, we identified deleted K. KU70 gene, which known be...
SummaryThe Atg12-Atg5 conjugate, which is formed by an ubiquitin-like conjugation system, essential to autophagosome formation, a central event in autophagy. Despite its importance, the molecular mechanism of conjugate formation has not been elucidated. Here, we report solution and crystal structures Atg10 Atg5 homologs from Kluyveromyces marxianus (Km), thermotolerant yeast. KmAtg10 comprises E2-core fold with characteristic accessories, including two β strands, whereas KmAtg5 domains...
ABSTRACT Auxotrophic mutants of the yeast Saccharomyces cerevisiae are usually isolated in haploid strains because isolation recessive mutations diploids is thought to be difficult due presence two sets genes. We show here that auxotrophic diploid industrial sake were routinely obtained by a standard mutant selection procedure following UV mutagenesis. His − , Met Lys Trp Leu Arg and Ura five strains, Kyokai no. 7, 9, 10, 701, 901, screening only 1,700 3,400 colonies from each treated...
The yeast protein Bem1p, which bears two src homology region 3 (SH3) domains, is involved in cell polarization. A Rho-type GTPase, Rho3p, the maintenance of polarity for bud formation, and rho3 defect suppressed by a high dose BEM1. Mutational analysis revealed that second SH3 domain from NH2 terminus (SH3-2) Bem1p important functions formation suppression defect. Boi2p, bound to SH3-2 was identified using two-hybrid system. Boi2p has proline-rich sequence critical displaying Boi2p-Bem1p...
A simple procedure for isolating yeast DNA suitable use as a template PCR amplification is described. SDS treatment alone sufficient extraction of chromosomal from cells. Cells colony are suspended in small volume (about 20 μL) 0.25% solution, mixed vigorously and centrifuged. The supernatant can be directly used after dilution to give an concentration less than 0.01% the final mixture.
Laccase is a multicopper-containing enzyme that catalyzes the oxidation of phenolic compounds. lcc1 cDNA coding for secretory laccase Pycnoporus coccineus was expressed under maltose inducible amyB promoter in Aspergillus oryzae and galactose GAL10 Saccharomyces cerevisiae. activities, which were undetectable absence copper, observed by increasing copper concentrations media both systems. The amounts secreted protein but not mRNA increased proportion to A. oryzae. extracellular activities...
The cloning of DNA fragments into vectors or host genomes has traditionally been performed using Escherichia coli with restriction enzymes and ligase homologous recombination-based reactions. We report here a novel method that does not require end processing recombination, but ensures highly accurate cloning. exploits the efficient non-homologous end-joining (NHEJ) activity yeast Kluyveromyces marxianus consists functional marker selection system. First, to demonstrate applicability NHEJ...