It is known that the nanoparticle-cell interaction strongly depends on physicochemical properties of investigated particles. In addition, medium density and viscosity influence colloidal behaviour nanoparticles. Here, we show how nanoparticle-protein interactions are related to particular characteristics particles, such as their stability, this significantly influences subsequent in vitro. Therefore, different surface charged superparamagnetic iron oxide nanoparticles were synthesized...

The challenge remains to reliably mimic human exposure high aspect ratio nanoparticles (HARN) via inhalation. Sophisticated, multi-cellular in vitro models are a particular advantageous solution this issue, especially when considering the need provide realistic and efficient alternatives invasive animal experimentation for HARN hazard assessment. By incorporating systematic test-bed of material characterisation techniques, specific air-liquid cell system with real-time monitoring...

A major goal of nanotechnology is the assembly nanoscale building blocks into functional optical, electrical, or chemical devices. Many these applications depend on an ability to optically electrically address single nanoparticles. However, positioning large numbers nanocrystals with nanometer precision a substrate for integration solid-state devices remains fundamental roadblock. Here, we report fast, scalable thousands nanoparticles using electrophoretic deposition. We demonstrate that...

Surface functionalization: Complete detoxification of gold nanorods was achieved by manipulating the position in stability map between surface-adsorbing polyethylene glycol (PEG) and destabilizing ethanol (see picture). This robust procedure complements studies related to effects shape when contemplating nanoparticle–cell interaction. As a service our authors readers, this journal provides supporting information supplied authors. Such materials are peer reviewed may be re-organized for...

When considering the inhalation of high-aspect ratio nanoparticles (HARN), characterization their specific interaction with lung cells is fundamental importance to help categorize potential hazard. The aim present study was assess cellulose nanocrystals (CNCs) a multicellular in vitro model epithelial airway barrier following realistic aerosol exposure. Rhodamine-labeled CNCs isolated from cotton (c-CNCs, 237 ± 118 × 29 13 nm) and tunicate (t-CNCs, 2244 1687 30 8 were found display different...

XPS is widely used to identify and quantify the elements present at surface of polymeric materials. The energy distribution photoelectrons emitted from these contains information about their chemical state, potentially allowing analyst specific functional groups. These groups may originate synthesis processing polymers, postsynthetic modifications such as grafting, or indeed be unrelated polymer (additives contaminants). Extracting reliable meaningful data not trivial relies on careful...

Abstract Although many nanoscale materials such as quantum dots and metallic nanocrystals exhibit size dependent optical properties, it has been difficult to incorporate them into or electronic devices because there are currently no methods for precise, large‐scale deposition of single nanocrystals. Of particular interest is the need control orientation since properties usually strongly anisotropic. Here a method based on electrophoretic (EPD) reported precisely assemble vertically oriented,...

Agglomeration of nanoparticles in biological fluids is a pervasive phenomenon that leads to difficulty the interpretation results from vitro exposure, primarily due differing particokinetics agglomerates nanoparticles. Therefore, well-defined small were designed possessed different particokinetic profiles, and their cellular uptake was compared computational model dosimetry. The approach used here paves way for better understanding impact agglomeration on nanoparticle-cell interaction.

Abstract The functionalization of gold nanorods (GNRs) with polymers is essential for both their colloidal stability and biocompatibility. However, a bilayer the toxic cationic surfactant cetyl trimethylammonium bromide (CTAB) adsorbed on complicates this process. Herein, we report strategy biocompatible GNRs hydrophobic polymeric precursor, polyvinyl acetate, which then transformed into its hydrophilic analogue, alcohol. This polymer was chosen due to well‐established biocompatibility,...

Owing to their ubiquitous distribution, expected beneficial effects and suspected adverse effects, nanoparticles are viewed as a double-edged sword, necessitating better understanding of interactions with tissues organisms. Thus, the goals present study were develop method generate quantitative data on nanoparticle entry into cells in culture exemplarily demonstrate usefulness this approach by analyzing impact size, charge various proteinaceous coatings particle internalization. N9...

Plasmon-mediated processes provide unique opportunities for selective photocatalysis, photovoltaics, and electrochemistry. Determining the influence of particle heterogeneity is an unsolved problem because often such introduce irreversible changes to nanocatalysts and/or their surroundings. The challenge lies in monitoring heterogeneous nonequilibrium dynamics via slow, serial methods that are intrinsic almost all spectral acquisition with suitable spatial resolution. Here, we present a new...

Size polydispersity is a common phenomenon that strongly influences the physicochemical properties of nanoparticles (NPs). We present an analytical approach universally applicable to characterizing optically anisotropic round NPs and determines directly number-averaged size distribution via depolarized dynamic light scattering (DDLS). To demonstrate, we use aqueous suspensions Au different sizes surface functionalization.

The impact of nanoparticles (NPs) upon biological systems can be fundamentally associated with their physicochemical parameters. A further often-stated tenet is the importance NP shape on rates endocytosis. However, given convoluted parameters concerning NP-cell interaction, it experimentally challenging to attribute any findings alone. Herein we demonstrate that shape, below a certain limit, which specific nanomedicine, not important for endocytosis spherocylinders by either epithelial or...

The assembly of nanoscale materials into arbitrary, organized structures remains a major challenge in nanotechnology. Herein, we report general method for creating 2D by combining top-down lithography with bottom-up chemical assembly. Under optimal conditions, the gold nanoparticles was achieved less than 30 min. Single nanoparticles, from 10 to 100 nm, can be placed predetermined patterns high fidelity, and higher-order generated consisting dimers or trimers. It is shown that nanoparticle...

Abstract Amino groups presented on the surface of nanoparticles are well‐known to be a predominant factor in formation protein corona and subsequent cellular uptake. However, molecular mechanism underpinning this relationship is poorly defined. This study investigates how amine type density affect association gold with cells vitro. Four specific poly(vinyl alcohol‐ co ‐ N ‐vinylamine) copolymers synthesized containing primary, secondary, or tertiary amines. Particle (i.e., uptake...

Oberflächenfunktionalisierung: Die vollständige Detoxifizierung von Goldnanostäben wurde durch Veränderung der Konzentrationen auf Oberfläche absorbiertem Polyethyenglycol und destabilisierendem Ethanol erreicht (siehe Bild). Diese robuste Methode ergänzt Untersuchungen zum Einfluss Form die Nanopartikel-Zell-Wechselwirkung. As a service to our authors and readers, this journal provides supporting information supplied by the authors. Such materials are peer reviewed may be re-organized for...

Abstract Controlled nanocrystal assembly is a pre‐requisite for incorporation of these materials into solid state devices. Many methods have been investigated which target precise positioning, high process controllability, scalability, and universality. However, most are unable to achieve all goals. Here, surface templated electrophoretic deposition (STED) presented as potential method wide variety nanocrystals. positioning range nanocrystals arbitrary spatial arrangements − including gold...

Epithelial tissue serves as an interface between biological compartments. Many in vitro epithelial cell models have been developed alternative to animal experiments answer a range of research questions. These are grown on permeable two-chamber systems; however, commercially available, polymer-based culture inserts around 10 μm thick. Since the basement membrane found systems is usually less than 1 thick, 10-fold thickness major limitation establishment realistic models. In this work, insert,...

Adapting computational particokinetic models to address the dosage of high-aspect ratio nanomaterials for <italic>in vitro</italic> nanoparticle toxicology assays involving submerged adherent cell cultures.

Characterizing the morphometric parameters of noble metal nanoparticles for sensing and catalysis is a persistent challenge due to their small size complex shape. Herein, we present an approach determine volume, surface area, curvature non-symmetric anisotropic using electron tomography design-based stereology without use segmentation tools or modeling particles. Finally, apply these aggregates estimate fractal dimension.

Abstract Unusual structural colors are demonstrated in thin‐film coatings due to a combination of optical interference and light scattering effects. These vivid concealed under ambient illumination but can be observed when is reflected from the film surface. The origin effect explored computationally it shown that, thin‐films lossless dielectrics coated on near‐perfect conductors, incident electromagnetic waves form standing waves. Electric field intensities at thin interfaces maximized for...