A5000481997- Antimicrobial Resistance in Staphylococcus
- Bacterial biofilms and quorum sensing
- Helicobacter pylori-related gastroenterology studies
- Galectins and Cancer Biology
- Biochemical and Structural Characterization
- Veterinary medicine and infectious diseases
- Bacteriophages and microbial interactions
- Bacterial Genetics and Biotechnology
- Bacterial Identification and Susceptibility Testing
- Clostridium difficile and Clostridium perfringens research
- Lymphoma Diagnosis and Treatment
- Antimicrobial Peptides and Activities
- Bacillus and Francisella bacterial research
- Corrosion Behavior and Inhibition
- Polyomavirus and related diseases
- Parvovirus B19 Infection Studies
- Escherichia coli research studies
- Viral Infections and Outbreaks Research
- Microbial Inactivation Methods
- SARS-CoV-2 and COVID-19 Research
- Metalloenzymes and iron-sulfur proteins
- Cutaneous lymphoproliferative disorders research
- Toxin Mechanisms and Immunotoxins
- CAR-T cell therapy research
New York University
2015-2024
Indiana University School of Medicine
2020
University of California, Santa Cruz
2008-2018
ABSTRACT Staphylococcus aureus is a versatile bacterial pathogen that can cause significant disease burden and mortality. Like other pathogens, S. must adapt to its environment produce virulence factors survive the immune responses evoked by infection. Despite importance of environmental signals for pathogenicity, only limited number these have been investigated in detail their ability modulate virulence. Here we show pyruvate, central metabolite, causes alterations overall metabolic flux...
The past two decades have witnessed an alarming expansion of staphylococcal disease caused by community-acquired methicillin-resistant
The pathogen
remains a causative agent for morbidity and mortality worldwide. This is in part result of antimicrobial resistance, highlighting the need to uncover novel antibiotic targets discover new therapeutic agents. In present study, we explored possibility that iron-sulfur (Fe-S) cluster synthesis viable target. RNA interference studies established Suf (
Therapy for bacteremia caused by Staphylococcus aureus is often ineffective, even when treatment conditions are optimal according to experimental protocols. Adapted subclones, such as those bearing mutations that attenuate agr -mediated virulence activation, associated with persistent infection and patient mortality.
ABSTRACT Staphylococcus aureus subverts innate defenses during infection in part by killing host immune cells to exacerbate disease. This human pathogen intercepts cues and activates a transcriptional response via the S. exoprotein expression (SaeR/SaeS [SaeR/S]) two-component system secrete virulence factors critical for pathogenesis. We recently showed that repressor CodY adjusts nuclease ( nuc ) gene SaeR/S, but mechanism remained unknown. Here, we identified two binding motifs upstream...
ABSTRACT Severe COVID-19 has been associated with coinfections bacterial and fungal pathogens. Notably, patients who develop Staphylococcus aureus bacteremia exhibit higher rates of mortality than those infected either pathogen alone. To understand this clinical scenario, we collected examined S. blood respiratory isolates from a hospital in New York City during the early phase pandemic both SARS-CoV-2+ SARS-CoV-2− patients. Whole genome sequencing these revealed broad phylogenetic diversity...
A key aspect underlying the severity of infections caused by Staphylococcus aureus is abundance virulence factors that pathogen uses to thwart critical components human immune response. One such mechanism involves destruction host cells cytolytic toxins secreted S. aureus, including five bicomponent leukocidins: PVL, HlgAB, HlgCB, LukED, and LukAB. Purified leukocidins can lyse ex vivo, systemic injections purified LukED or HlgAB acutely kill mice. Here, we describe generation...
Staphylococcus aureus is a Gram-positive opportunistic pathogen that causes superficial and invasive infections in the hospital community. High mortality from infection emphasizes need for improved methods prevention treatment. Although S. possesses an arsenal of virulence factors contribute to evasion host defenses, few studies have examined long-term humoral B-cell responses. Adults with acute-phase skin soft tissue were recruited; blood samples obtained; isolates, including...
ABSTRACT Here we undertook to identify colonization and gastric disease-promoting factors of the human pathogen Helicobacter pylori as genes that were induced in response stomach environment. Using recombination-based vivo expression technology (RIVET), identified six promoters host compared laboratory conditions. Three these promoters, designated P ivi10 , ivi66 ivi77 regulate H. may use interact with other microbes or host. likely regulates mobA mobB mobD genes, which have potential roles...
The human pathogen Helicobacter pylori employs a diverse collection of outer membrane proteins to colonize, persist, and drive disease within the acidic gastric environment. In this study, we sought elucidate function host-induced gene HP0289, which encodes an uncharacterized protein. We first generated isogenic H. mutant that lacks HP0289 found has colonization defect in single-strain infections is greatly outcompeted mouse coinfection experiments with wild-type pylori. Furthermore, used...
ORIGINAL RESEARCH article Front. Cell. Infect. Microbiol., 20 April 2012Sec. Molecular Bacterial Pathogenesis Volume 2 - 2012 | https://doi.org/10.3389/fcimb.2012.00046
Staphylococcus aureus is a major human pathogen that imposes great burden on the health care system. In development of antistaphylococcal modalities intended to reduce staphylococcal disease, it imperative select appropriate models S. strains when assessing efficacy novel agents. Here, using whole-genome sequencing, we reveal commonly used strain Newman D2C from American Type Culture Collection (ATCC) contains mutations render essentially avirulent. Importantly, often inaccurately referred...
Bacterial flagella are rotary nanomachines that contribute to bacterial fitness in many settings, including host colonization. The flagellar motor relies on the multiprotein motor-switch complex govern flagellum formation and rotational direction. Different bacteria exhibit great diversity their motors. One such variation is exemplified by apparatus of gastric pathogen Helicobacter pylori, which carries an extra switch protein, FliY, along with more typical FliG, FliM, FliN proteins. All...
The human pathogen Helicobacter pylori uses the host receptor α5β1 integrin to trigger inflammation in cells via its cag pathogenicity island (cag PAI) type IV secretion system (T4SS). Here, we report that H. ImaA protein (HP0289) decreases action of PAI T4SS tempering bacterium's interaction with integrin. Previously, imaA-null mutants were found induce an elevated inflammatory response was dependent on T4SS; here extend those findings show is independent CagA effector protein. To...
OBJECTIVES/GOALS: We recently identified a CA-MRSA strain in Brooklyn, New York (USA300-BKV) causing an outbreak of severe skin infections predominantly healthy children. The evolution USA300-BKV included acquisition novel prophage, and our objective is to identify the prophage-encoded gene(s) mechanism responsible for increased bacterial virulence. METHODS/STUDY POPULATION: deleted candidate genes from mosaic block phage-encoded that have been shown enhance virulence murine infection model....