A5000188264- Enzyme Production and Characterization
- Insect Resistance and Genetics
- Epigenetics and DNA Methylation
- RNA modifications and cancer
- Biofuel production and bioconversion
- RNA Research and Splicing
- Malaria Research and Control
- Antimicrobial Resistance in Staphylococcus
- Neuropeptides and Animal Physiology
- Microbial Metabolites in Food Biotechnology
- Insect Pest Control Strategies
- Receptor Mechanisms and Signaling
- Enzyme Catalysis and Immobilization
- Tuberculosis Research and Epidemiology
- Bacteriophages and microbial interactions
- Bacterial biofilms and quorum sensing
- Genetic factors in colorectal cancer
- MicroRNA in disease regulation
- vaccines and immunoinformatics approaches
- Cancer Genomics and Diagnostics
- Viral Infectious Diseases and Gene Expression in Insects
- RNA and protein synthesis mechanisms
- Computational Drug Discovery Methods
- Mitochondrial Function and Pathology
- Genomics and Rare Diseases
All India Institute of Medical Sciences
2018-2024
All India Institute of Medical Sciences Bhopal
2018-2024
All India Institute of Medical Sciences Raipur
2018-2024
Chandra Shekhar Azad University of Agriculture and Technology
2023
Institute of Medical Sciences
2023
University of Delhi
2006-2022
Indian Institute of Technology Guwahati
2018-2022
Ambedkar University Delhi
2019-2022
New York University
2012-2019
National Institutes of Health
1993-2018
Background Mutations and promoters' methylation of a set candidate cancer genes (CAN genes) are associated with progression colorectal (CRC). We hypothesized that these genes' promoters inactivated through epigenetic silencing may show different profile in high-risk populations. investigated the status CAN gene CHD5 protein expression African American CRC tissue microarrays (TMA) using immunohistochemical staining. Methodology/Principal Findings The promoter was studied by...
Staphylococcal pathogenicity islands (SaPIs) carry superantigen and resistance genes are extremely widespread in Staphylococcus aureus other Gram-positive bacteria. SaPIs represent a major source of intrageneric horizontal gene transfer stealth conduit for intergeneric transfer; they phage satellites that exploit the life cycle their temperate helper phages with elegant precision to enable rapid replication promiscuous spread. also interfere reproduction, blocking plaque formation, sharply...
NAC proteins are plant-specific transcription factors (TFs). Although they play a pivotal role in regulating distinct biological processes, TFs maize yet to be investigated comprehensively. Within the genome, we identified 152 putative domain-encoding genes (ZmNACs), including eight membrane-bound members, by systematic sequence analysis and physically mapped them onto ten chromosomes of maize. In silico ZmNACs comparison with similar other plants such as Arabidopsis, rice, soybean, revealed...
ABSTRACT The extended rod-like Plasmodium falciparum circumsporozoite protein (CSP) is comprised of three primary domains: a charged N terminus that binds heparan sulfate proteoglycans, central NANP repeat domain, and C containing thrombospondin-like type I (TSR) domain. Only the last two domains are incorporated in RTS,S, leading malaria vaccine phase 3 trials that, to date, protects about 50% vaccinated children against clinical disease. A seroepidemiological study indicated N-terminal...
The past two decades have witnessed an alarming expansion of staphylococcal disease caused by community-acquired methicillin-resistant
Elucidation of the principal targets action antimalarial drug artemisinin is an ongoing pursuit that important for understanding this and development more potent analogues. We have examined chemical reaction Hb with artemisinin. The protein-bound haem in has been found to react much faster than case free haem. It appears uptake accumulation into food vacuole, together preferred reactivity Hb, may make primary target artemisinin's action. Both monoalkylated (HA) dialkylated (HAA) derivatives...
Colorectal cancer develops through genetic, epigenetic, and environmental events that result in uncontrolled cell proliferation. incidence mortality is higher African Americans (AA) than the general population. Here, we carried out a molecular analysis of sporadic colorectal tumors from AAs to investigate possible explanations for observed disparities.A total 222 AA were analyzed microsatellite instability (MSI) protein expression two DNA mismatch repair genes, MLH1 MSH2, by...
Therapy for bacteremia caused by Staphylococcus aureus is often ineffective, even when treatment conditions are optimal according to experimental protocols. Adapted subclones, such as those bearing mutations that attenuate agr -mediated virulence activation, associated with persistent infection and patient mortality.
Focal cortical dysplasia (FCD) is one of the most common pathologies associated with drug-resistant epilepsy (DRE). The pharmacological targets remain obscured, as molecular mechanisms underlying FCD are unclear. Implications epigenetically modulated aberrant gene expression in disease progression reported various DRE except FCD. Here we performed genome-wide CpG-DNA methylation profiling by methylated DNA immunoprecipitation (MeDIP) microarray and RNA sequencing (RNAseq) on tissues resected...
Cysteine proteases (falcipains) of Plasmodium falciparum are potential targets for antimalarial chemotherapy, since they have been shown to be involved in important cellular functions such as hemoglobin degradation and invasion/rupture red blood cells during parasite life cycle. The role falcipain-1 at the asexual stages still remains uncertain. This is mainly due a lack methods prepare this protein an active form. In order obtain biologically falcipain-1, number constructs were designed...
Background Colon cancer is one of the leading causes related deaths. Its impact on African Americans (AAs) higher than in general population both incidence and mortality from disease. aggressiveness AAs as well non-frequent check-ups follow up this have been proposed ways to explain observed discrepancies. These facts made detection early carcinogenesis markers a priority. Materials Methods Here, we analyzed 50 colon adenomas AA patients for microsatellite instability (MSI) methylation...
Background Protein-protein interactions play a crucial role in enabling pathogen to survive within host. In many cases the involve complex of proteins rather than just two given proteins. This is especially true for pathogens like M. tuberculosis that are able successfully inhospitable environment macrophage. Studying such detail may help developing small molecules either disrupt or augment interactions. Here, we describe development an E. coli based bacterial three-hybrid system can be used...
The agr system of Staphylococcus aureus promotes invasion host tissues, and as expected, agents that block quorum sensing have anti-infective properties. Paradoxically, agr-defective mutants are frequently recovered from patients, especially those persistently infected with S. We found an deficiency increased survival cultured bacteria during severe stress, such treatment gentamicin, ciprofloxacin, heat, or low pH. With daptomycin, deletion decreased survival. Therefore, activity can be...
The secretory proteins of Mycobacterium tuberculosis (M. tuberculosis) have been known to be involved in the virulence, pathogenesis as well proliferation pathogen. Among this set, many hypothesized play a critical role at genesis onset infection, primary site which is invariably human lung.During our efforts isolate potential binding partners key M. from lung protein library, we isolated peptides that strongly bound virulence determinant Esat6. All were less than fifty amino acids length...
Abstract Focal cortical dysplasia (FCD) is a malformation of the cerebral cortex with poorly-defined epileptogenic zones (EZs), and poor surgical outcome in FCD associated inaccurate localization EZ. Hence, identifying novel markers to aid EZ patients very much needed. High-throughput gene expression studies samples have potential uncover molecular changes underlying process identify for delineating For this purpose, we, first time performed RNA sequencing surgically resected paired tissue...