A5103258165- Parvovirus B19 Infection Studies
- Viral-associated cancers and disorders
- Cytomegalovirus and herpesvirus research
- Prenatal Screening and Diagnostics
- Pregnancy and preeclampsia studies
- Respiratory viral infections research
- Maternal and fetal healthcare
- Herpesvirus Infections and Treatments
- SARS-CoV-2 detection and testing
- Neonatal Respiratory Health Research
- Viral Infections and Immunology Research
- Congenital Anomalies and Fetal Surgery
- Viral gastroenteritis research and epidemiology
- Dermatological and COVID-19 studies
- Bacterial Identification and Susceptibility Testing
- Cardiovascular Issues in Pregnancy
- Acute Lymphoblastic Leukemia research
- Intestinal Malrotation and Obstruction Disorders
- Polyomavirus and related diseases
- Biosensors and Analytical Detection
- Tumors and Oncological Cases
- Clinical Laboratory Practices and Quality Control
- Preterm Birth and Chorioamnionitis
- Epigenetics and DNA Methylation
- Soft tissue tumor case studies
Emory University
2014-2024
Children's Healthcare of Atlanta
2015-2024
Rogers (United States)
1999-2022
The University of Texas Southwestern Medical Center
2002-2014
Children's Medical Center
1999-2013
Primary Children's Hospital
2006
University of Utah
2006
Stanford Medicine
2006
Southwestern Medical Center
1996-2005
National Institute of Allergy and Infectious Diseases
2004
Context Evolution of polymerase chain reaction testing for infectious pathogens has occurred concurrent with a focus on value-based medicine. Objective To determine if implementation the FilmArray rapid respiratory panel (BioFire Diagnostics, Salt Lake City, Utah) (hereafter RRP), shorter time to test result and expanded panel, results in different outcomes children admitted hospital an acute tract illness. Design Patient were compared before RRP (November 1, 2011, January 31, 2012) versus...
While there has been significant progress in the development of rapid COVID-19 diagnostics, as pandemic unfolds, new challenges have emerged, including whether these technologies can reliably detect more infectious variants concern and be viably deployed non-clinical settings "self-tests". Multidisciplinary evaluation Abbott BinaxNOW Ag Card (BinaxNOW, a widely used antigen test, included limit detection, variant test performance across different age-groups, usability with...
Respiratory syncytial virus (RSV) infection is associated with acute morbidity (e.g., pneumonia and airway obstruction [AO]) long-term complications hyperresponsiveness [AHR]). We present a comprehensive evaluation of the chronic phases RSV respiratory tract infection, using mouse model.BALB/c mice were inoculated monitored for 154 days. loads cytokines measured in bronchoalveolar lavage (BAL) samples. Pneumonia severity was assessed standard histopathologic score, pulmonary function...
Background Inflammatory diseases of the heart, including myocarditis and cardiac transplant rejection, are important causes morbidity mortality in children. Although viral infection may be suspected either these clinical conditions, definitive etiology is often difficult to ascertain. Furthermore, histology identical for both disorders. Coxsackievirus has long been considered most common cause myocarditis; however, we previously demonstrated by polymerase chain reaction (PCR) analysis that...
Objectives: Our aim was to evaluate the utility of color flow mapping in prediction placental myometrial invasion women with Cesarean delivery. Methods Ultrasound performed on implantations potential proximity hysterotomy scar. The smallest thickness measured under placenta degree attenuation this area and note made unusual vascular lakes. Results Two hundred fifteen placentas prior scar underwent Doppler mapping. Of 20 previa delivery, 15 had hysterectomy for bleeding complications nine...
Human herpesviruses can cause acute diseases such as chicken pox or mononucleosis, but also may reactivate during immunosuppression and result in severe life-threatening illnesses shingles lymphoproliferative disorders. We report the development validation of a quantitative PCR method to measure viral burden for all eight human (HSV1, HSV2, VZV, EBV, CMV, HHV6, HHV7, KSHV) patients' samples. The uses an internal standard that is coamplified with target, allowing quantification genomes...
<h3>Objective.</h3> —To evaluate the clinical utility of perinatal autopsy in determining cause a death. <h3>Design.</h3> —Retrospective observational survey. <h3>Setting.</h3> —University-affiliated, private, tertiary care hospital, limited to obstetrics, gynecology, and neonatology. <h3>Subjects.</h3> —All fetal deaths neonatal from 1990 1991 at Women Infants Hospital, Providence, RI, were reviewed. Fetal with gestational age less than 20 weeks occurring more 48 hours after birth excluded....
Because chronic Mycoplasma pneumoniae respiratory infection is hypothesized to play a role in asthma, the potential of M. establish with associated pulmonary disease was investigated murine model. BALB/c mice were intranasally inoculated once and examined at 109, 150, 245, 368, 530 days postinoculation. detected bronchoalveolar lavage fluid by culture or PCR 70 22% 109 postinoculation, respectively. Lung histopathology normal up 368 At days, however, 78% demonstrated abnormal characterized...
The purpose of our study was to confirm reports an association human papillomavirus (HPV) with neonatal giant cell hepatitis (GCH) and biliary atresia (BA), expand these studies include cytomegalovirus (CMV), Epstein-Barr virus (EBV), herpesvirus 6 (HHV6), parvovirus B19 (PVB19). Frozen hepatic tissue available for polymerase chain reaction (PCR) analysis in 19 cases GCH or BA 8 controls. Nested PCR detect HPV types 6, 16, 18, 33 followed by 32P hybridization generic probes. a...
ABSTRACT The GeneXpert Dx system (Cepheid, Sunnyvale, CA) is a fully integrated and automated nucleic acid sample preparation, amplification, real-time detection system. It consists of an instrument, personal computer, disposable fluidic cartridges. analytical sensitivity specificity the enterovirus assay (GXEA) were determined with panel 63 different serotypes 24 other microorganisms, respectively. potential for blood, hemoglobin, white blood cells, excess protein to interfere was also...
ABSTRACT Because Mycoplasma pneumoniae is hypothesized to play an important role in reactive airway disease/asthma, a comprehensive murine model of M. lower respiratory infection was established. BALB/c mice were intranasally inoculated once with and sacrificed at 0 42 days postinoculation. All became infected developed histologic evidence acute pulmonary inflammation, which cleared by 28 By contrast, persisted the tract for entire studied. Tumor necrosis factor alpha, gamma interferon,...
ABSTRACT Nucleic acid amplification tests (NAATs) for enterovirus RNA in cerebrospinal fluid (CSF) have emerged as the new gold standard diagnosis of enteroviral meningitis, and their use can improve management decrease costs caring children with meningitis. The Xpert EV assay (Cepheid, Sunnyvale, CA) is a rapid, fully automated real-time PCR test detection that was approved by U.S. Food Drug Administration vitro diagnostic March 2007. In this multicenter trial we established clinical...
Background Matrix-assisted laser desorption ionisation–time of flight mass spectrometry (MALDI-TOF MS) has been described as a rapid, accurate method for bacterial identification. Aims To investigate the ability technique, using unamended database supplied with system, to identify bacteria commonly isolated in cystic fibrosis (CF) patients. Methods Organisms from CF patients identified by MALDI-TOF MS were compared conventional phenotypic and genotypic analyses. For MS, direct colony...
Sharma, Shiv K.; Rogers, Beverly B.; Alexander, James M.; McIntire, Donald D.; Leveno, Kenneth J. Author Information
ABSTRACT Background DICER1 mutations, though infrequent, are encountered on preoperative molecular testing of indeterminate adult and pediatric thyroid fine‐needle aspiration (FNA) specimens. Yet, published cytomorphologic features ‐altered lesions limited. Cytomorphological were examined in a multipractice FNA cohort with clinical, radiological, histologic data. Methods The comprised 18 FNAs, 14 having slides available eight corresponding surgical resections. Smears, ThinPrep,...
Chronic villitis is a histologic diagnosis that may be either associated with infection, or termed of undetermined etiology (VUE). The lymphocytic infiltrate in VUE has been reported to consist maternal lymphocytes, but the origin infectious not identified. purpose our study was compare vs. fetal infiltrating lymphocytes and syphilitic villitis, expand immunophenotypic data provided by previous studies. Paraffin-embedded placentas from four males two vil litis were subjected fluorescence...