A5058670656- Photosynthetic Processes and Mechanisms
- Spectroscopy and Quantum Chemical Studies
- Advanced Proteomics Techniques and Applications
- Photoreceptor and optogenetics research
- Light effects on plants
- Glycosylation and Glycoproteins Research
- Mass Spectrometry Techniques and Applications
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Electrochemical Analysis and Applications
- Algal biology and biofuel production
- Retinal Development and Disorders
- Bryophyte Studies and Records
- Melanoma and MAPK Pathways
- Lysosomal Storage Disorders Research
- Microbial Metabolic Engineering and Bioproduction
- Analytical Chemistry and Sensors
- Nitric Oxide and Endothelin Effects
- Mitochondrial Function and Pathology
- Adenosine and Purinergic Signaling
- Plant responses to elevated CO2
- Pancreatic and Hepatic Oncology Research
- Smart Materials for Construction
- Angiogenesis and VEGF in Cancer
- Plant Stress Responses and Tolerance
- Advanced biosensing and bioanalysis techniques
University of Ottawa
2024
BioMarin (United States)
2018-2020
Institute of Cancer Research
2012-2016
Institute of Cancer Research
2012-2014
University College London
2006-2014
Allergan (United States)
2012
The Christie NHS Foundation Trust
2012
Cancer Research UK Manchester Institute
2012
University of Manchester
2012
Molecular Oncology (United States)
2012
We generated cell lines resistant to BRAF inhibitors and show that the EGF receptor (EGFR)-SRC family kinase (SFK)-STAT3 signaling pathway was upregulated in these cells. In addition driving proliferation of cells, this also stimulated invasion metastasis. EGFR cooperated with block growth cells vitro vivo, monotherapy broad specificity tyrosine inhibitor dasatinib blocked metastasis vivo. analyzed tumors from patients intrinsic or acquired resistance vemurafenib observed increased SFK...
Reproducible, comprehensive phosphopeptide enrichment is essential for studying phosphorylation-regulated processes. Here, we describe the application of hyper-porous magnetic TiO2 and Ti-IMAC microspheres uniform automated enrichment. Combining with a particle-handling robot enables rapid (45 min), reproducible (r2 ≥ 0.80) high-fidelity (>90% purity) purification in 96-well format. Automated demonstrates synthetic recovery across 2 orders magnitude, "well-to-well" quantitative...
Biotransformation pathways and the potential for drug-drug interactions of orally active antifungal terbinafine were characterized using human liver microsomes recombinant cytochrome P-450s (CYPs). The metabolites represented four major pathways: 1) N-demethylation, 2) deamination, 3) alkyl side chain oxidation, 4) dihydrodiol formation. Michaelis-Menten kinetics revealed mean K(m) values ranging from 4.4 to 27.8 microM, V(max) 9.8 82 nmol/h/mg protein. At least seven CYP enzymes are...
Biliary tract cancer (BTC) and benign biliary strictures can be difficult to differentiate using standard tumour markers such as serum carbohydrate antigen 19-9 (CA19-9) they lack diagnostic accuracy.Two-dimensional difference gel electrophoresis tandem mass spectrometry were used profile immunodepleted samples collected from cases of BTC, primary sclerosing cholangitis (PSC), immunoglobulin G4-associated healthy volunteers. The levels one candidate protein, leucine-rich α-2-glycoprotein...
Purpose Ovarian cancer is a devastating disease and biomarkers for its early diagnosis are urgently required. Serum may be valuable source of that revealed by proteomic profiling. Herein, complementary serum protein profiling strategies were employed discovery could discriminate cases malignant benign ovarian cancer. Experimental design Identically collected processed samples from 22 invasive epithelial cancer, 45 neoplasms, 64 healthy volunteers subjected to immunodepletion equalization...
Mass spectrometry reveals an EPHA2-based mechanism for the regulation of metastatic cancer cell extravasation.
Using an integrated approach incorporating proteomics, metabolomics and published mRNA data, we have investigated the effects of hydrogen peroxide on wild type a Sty1p-deletion mutant fission yeast Schizosaccharomyces pombe. Differential protein expression analysis based modification proteins with matched fluorescent labelling reagents (2-D-DIGE) is foundation quantitative proteomics approach. This study identifies 260 differentially expressed isoforms from 2-D-DIGE gels using MALDI MS...
The present study was performed to investigate the effect of crocin on blue light- and white light-induced rod cone death in primary retinal cell cultures.Primary cultures were prepared from primate bovine retinas. Fifteen-day-old exposed actinic light or fluorescent for 24 hours. Cultures treated by addition different concentrations hours before exposure 8 after exposure. kept dark used as controls. Green nucleic acid stain assay evaluate death. Rods cones immunolabeled with specific...
Hypoxia is present in most solid tumors and clinically correlated with increased metastasis poor patient survival. While studies have demonstrated the role of hypoxia hypoxia-regulated proteins cancer progression, no attempts been made to identify using quantitative proteomics combined MALDI-mass spectrometry imaging (MALDI-MSI). Here we a comprehensive hypoxic proteome study are first investigate changes situ tumor samples. In vitro mass analysis was performed on breast cells stable isotope...
Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) is an endogenous secreted peptide and, in preclinical studies, preferentially induces apoptosis tumor cells rather than normal cells. The acquisition of resistance exposed to TRAIL or its mimics limits their clinical efficacy. Because kinases are intimately involved the regulation apoptosis, we systematically characterized signaling. Using RNA interference (RNAi) loss-of-function and cDNA overexpression screens, identified 169...
At the early stages of carcinogenesis, transformation occurs in single cells within tissues. In an epithelial monolayer, such mutated are recognized by their normal neighbors and often apically extruded. The apical extrusion requires cytoskeletal reorganization cell shape changes, but it is poorly understood what molecular switches involved regulation these processes. Here, using SILAC-based quantitative mass spectrometry we have identified proteins that modulated transformed upon...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTThe Heat Capacity, Entropy and Content of Sodium Amide from 15 to 300°K. The Thermodynamics Ion in Liquid Ammonia1L. V. Coulter, J. R. Sinclair, A. G. Cole, C. RoperCite this: Am. Chem. Soc. 1959, 81, 12, 2986–2989Publication Date (Print):June 1, 1959Publication History Published online1 May 2002Published inissue 1 June 1959https://doi.org/10.1021/ja01521a017Request reuse permissionsArticle Views208Altmetric-Citations23LEARN ABOUT THESE...
Several recent studies have suggested that the spatial tuning of retinal ganglion cells may be a more complex process than previously thought. The working hypothesis for many years was shaped by operations performed in first synaptic layer retina, but work shows second layer, involving amacrine cells, also play significant role (Cook and McReynolds, 1998; Taylor, 1999; Flores-Herr et al., 2001). Although it is clear are involved, precise roles different subtypes aspects not yet been...
Abstract Objective Neuronal ceroid lipofuscinosis type 2 (CLN2 disease) is a rare, progressive, fatal neurodegenerative pediatric disorder resulting from deficiencies of the lysosomal enzyme tripeptidyl peptidase 1 that are caused by mutations in TPP1 . Identifying biomarkers CLN2 disease progression will be important assessing efficacy therapeutic interventions for this disorder. Neurofilament light an intrinsic component healthy neurons; elevated circulating extracellular neurofilament...
The aim of this discovery study was the identification peptide serum biomarkers for detecting biliary tract cancer (BTC) using samples from healthy volunteers and benign cases disease as control groups. This work based on hypothesis that cancer-specific exopeptidases exist their activities in can generate cancer-predictive fragments circulating proteins during coagulation.This case used a semi-automated platform incorporating polypeptide extraction linked to matrix-assisted laser...
The photosynthetic evolution of oxygen by isolated chloroplasts Spinacia oleracea L. was studied using a modulated electrode. enhancement effect, measured as the increase in relative quantum yield 650-nanometer light due to presence 710-nanometer light, profoundly influenced concentration inorganic cations bathing medium. Chloroplast fragments immersed solution containing very low MgCl2 or KCl, did not display but could be made do so raising 3 mm, that KCl 35 mm. This change properties...