Shape-controlled synthesis of metal nanostructures has opened many new possibilities to design ideal building blocks for future nanodevices. In this work, types monodisperse silver nanoplates with complex shapes, namely, a disklike shape and flowerlike were controllably synthesized in high yield by reducing [Ag(NH3)2]+ ascorbic acid the presence seed at room temperature. Unlike previous methods synthesizing cetyltrimethylammonium bromide (CTAB) micelles, use precursor [Ag(NH3)2]+, other than...

How particles aggregate into an interesting dendritic structure has been the object of research for many years because its importance in understanding physical processes involved and designing novel materials. In this work, we first time describe oriented attachment-based assembly mechanism formation different types silver nanostructures at room temperature. It is found that concentration both AgNO3 p-aminoazobenzene (PA) molecules a significant effect on growth these nanostructures....

On the substrate carrying a sub-wavelength grating covered with thin metal layer, fluorescent dye-labeled cell was observed by fluorescence microscope. The intensity more than 20 times greater that on an optically flat glass substrate. Such great enhancement from labeled cells bound to due excitation coupled surface plasmon resonance. application of two-dimensional detection and microscopy appears offer promising method taking highly sensitive images.

Two types of plasmonic silver- and gold-coated grating biosensor chips (plasmonic chip) were applied in the detection α-fetoprotein (AFP) with a sandwich imunoassay surface plasmon field-enhanced fluorescence. On chip, unlabeled marker immunoassay was first quantitatively detected over wide range between 10(-12) 10(-8) g/mL. The affinity constants AFP anti-AFP antibody, which obtained by fitting experimental data to Langmuir isotherm adsorption curve, 1 × 10(8) g(-1) mL regardless kind metal...

Static polarizabilities and hyperpolarizabilities have been calculated for the furan homologues including (C4H4O), thiophene (C4H4S), selenophene (C4H4Se), tellurophene (C4H4Te), at Hartree−Fock various correlation levels by ab initio molecular orbital methods. The influence of substitution with heavy atoms on properties was examined theoretically. In calculations molecules Se Te atoms, contribution core electrons in estimated using effective potential (ECP) With calculation molecule,...

Two single‐stranded DNAs consisting of complementary base pairs except for one mismatching pair (MM1) can form double‐stranded DNA by molecular recognition. This type duplex is not as stable that formed MM0. In order to add a better understanding the physical mechanism hybridization and dissociation processes at sensor (chip) surfaces, we studied kinetics MM1 surface plasmon fluorescence spectroscopy. Target strands labelled with fluorescent molecule Cy5 5′ end hybridizing surface‐attached...

Abstract A two‐dimensional (2D) plasmonic coupling nanostructure for enhanced fluorescence observation using a microscope is presented. The substrate contained periodically assembled nanohole arrays with pitch of 400 nm and depth 25 nm. In comparison one‐dimensional (1D) gratings, this new presented an excellent surface plasmon ability to illuminate light from all directions. Under optical microscope, enhancement in the intensity up 100 times compared plain glass slide was observed. markedly...

Abstract The ability to precisely control the pattern of metallic structures at micro‐ and nanoscale for surface plasmon coupling has been demonstrated be essential signal enhancement in fields such as fluorescence surface‐enhanced Raman scattering. In present study, a series silver coated gratings with tailored duty ratio depth periodical pitch 400 nm are designed implemented. influence grating profile on plasmonic properties corresponding factor investigated by angular scanning measurement...

Polydopamine (PDA) thin films are introduced to the surface modification of biosensor surfaces utilizing plasmon enhanced fluorescence spectroscopy (SPFS) as linker layer capture antibody on sensor surfaces. The can be directly attached without using any coupling agent by functionalizing gold with PDA films. coating is performed a single-step preparation process applying dopamine solution surface, which requires an extremely short incubation time (10 min). real-time in situ measurement...

This article reports the designed preparation of two different kinds novel porous metal nanostructured films, namely, an ordered macroporous Au/Ag film and hollow film. Different from previous reports, presently proposed method can be conveniently used to control structures by simply varying experimental conditions. The morphology these films has been characterized scanning electron microscopy (SEM), their performance as surface-enhanced Raman scattering (SERS) substrates evaluated using...

The grating substrate covered with a metal layer, plasmonic chip, and bispecific antibody can play key role in the sensitive detection of marker protein an immunosensor, because provision enhanced fluorescence signal preparation sensor surface densely modified capture antibody, respectively. In this study, one tumor markers, soluble epidermal growth factor receptor (sEGFR), was selected as target to be detected. ZnO- silver-coated chip precise regularity appropriate duty ratio periodic...

A plasmonic chip was applied to live cancer cell imaging. The epithelial adhesion molecule (EpCAM) is a surface marker that can be used classify breast lines into distinct differentiation states. EpCAM and the nuclei of two kinds living cells, MDA-MB231 MCF-7, were stained with allophycocyanin (APC)-labeled anti-EpCAM antibody 4',6-diamidino-2-phenylindole (DAPI), respectively, cells scattered on either (metal-coated wavelength-scale grating substrate) or control glass slide. Multicolor...

A synthetic polymer ligand-grafted plasmonic chip was fabricated and demonstrated a highly sensitive detection of C-reactive protein (CRP) by grating-coupled surface plasmon field-enhanced fluorescence.

A plasmonic biosensor chip of silver-coated PMMA grating with a zinc oxide (ZnO) overlayer is fabricated for surface plasmon field-enhanced fluorescence (SPF) detection Cy5-labeled green fluorescent protein (GFP). bispecific antibody (anti-GFP x anti-ZnO antibody) prepared in our lab densely immobilized on the sensor GFP detection. The sensitivity biosensors improved due to packed antibodies and ZnO-coating that suppresses nonspecific adsorption quenching. With ZnO-coated chip, 10 pM can be...

A plasmonic dish was fabricated as a novel cell-culture for in situ sensitive imaging applications, which the cover glass of glass-bottomed replaced by grating substrate coated with film silver. Neuronal cells were successfully cultured over period more than 2 weeks dish. The fluorescence images their including dendrites simply observed using conventional microscope. from neuronal growing along surface enhanced plasmon resonance field. Under an epi-fluorescence microscope and employing...

In this study, the utility of plasmonic coloration on silver nanodome arrays for sensitive and quantitative detection biomolecules using a smartphone-based sensor is proposed. particular, analysis DNA hybridization was achieved hue angle in HSV color space obtained from photograph sensing spot taken smartphone camera. Silver gold consisting polystyrene bead layer covered with thin metal film can be created over large area by bottom-up fabrication process. The exhibited unique colorations...

A plasmonic chip, which is a grating substrate coated with metal films, was fabricated and applied to grating-coupled surface-plasmon-field-enhanced fluorescence imaging (GC-SPFI). With the enhanced excited by surface plasmon field on interleukin-6 (IL-6), marker protein of lifestyle-related diseases, measured using sandwich assay system. IL-6 quantitatively determined be up 2 pg/mL GC-SPFI. The detection sensitivity for superior that an enzyme-linked immunosorbent assay, GC-SPFI shown valid...

A rapid and sensitive detection of a cancer marker, neuron specific enolase (NSE), is demonstrated by using disposable silver plasmonic chip functionalized with mussel-inspired polydopamine (PDA) coating. consisting diffraction grating coated thin film used for the excitation propagating surface plasmon resonance through rear-side coupling method. Simple quick bio-functionalization sensor performed PDA coating which requires 20 min deposition, allows direct attachment capture antibody...

A Bull's eye-plasmonic chip composed of concentric circles was applied to enhanced fluorescence microscopy. Among one dimensional (1-D), 2-D, and eye periodic structures, the provided most intensity under epi-fluorescence microscope, because incident light through objective lens with all azimuthal angles can be effectively surface plasmon resonance- field (excitation field) plasmon-enhanced emission also collected. In observation a single nanoparticle, images for microsphere ϕ 2 μm...