A5068781825- Monoclonal and Polyclonal Antibodies Research
- Microtubule and mitosis dynamics
- Advanced biosensing and bioanalysis techniques
- Biofuel production and bioconversion
- Glycosylation and Glycoproteins Research
- Micro and Nano Robotics
- Microbial Metabolic Engineering and Bioproduction
- Bacteriophages and microbial interactions
- Protein purification and stability
- Advanced Cellulose Research Studies
- Genomics and Phylogenetic Studies
- Advanced Biosensing Techniques and Applications
- Enzyme Production and Characterization
- RNA and protein synthesis mechanisms
- Molecular Junctions and Nanostructures
- Plasmonic and Surface Plasmon Research
- Diatoms and Algae Research
- Viral Infectious Diseases and Gene Expression in Insects
- Biochemical and Structural Characterization
- Transgenic Plants and Applications
- RNA Interference and Gene Delivery
- CRISPR and Genetic Engineering
- Catalysis for Biomass Conversion
- CAR-T cell therapy research
- Galectins and Cancer Biology
Tohoku University
2016-2025
Sendai University
2015-2018
Graduate School USA
2015-2016
Institut de Biologie et de Chimie des Protéines
2016
Université Claude Bernard Lyon 1
2016
Nagaoka University of Technology
2008-2015
Hirosaki University
2013
Nagaoka University
1988-2009
Sumitomo Metal Mining (Japan)
1991
Hokkaido University
1987-1988
Molecular evolution based on mutagenesis is widely used in protein engineering. However, optimal proteins are often difficult to obtain due a large sequence space. Here, we propose novel approach that combines molecular with machine learning. In this approach, conduct two rounds of where an initial library variants train machine-learning model guide for the second-round library. This enables us prepare small suited screening experiments high enrichment functional proteins. We demonstrated...
To develop a Trichoderma reesei strain appropriate for the saccharification of pretreated cellulosic biomass, recombinant T. strain, X3AB1, was constructed that expressed an Aspergillus aculeatus β-glucosidase 1 with high specific activity under control xyn3 promoter. The culture supernatant from X3AB1 grown on 1% Avicel as carbon source had 63- and 25-fold higher against cellobiose compared to parent PC-3-7 expressing endogenous I, respectively. Further, xylanase 30% lower than due absence...
Machine learning (ML) is becoming an attractive tool in mutagenesis-based protein engineering because of its ability to design a variant library containing proteins with desired function. However, it remains unclear how ML guides directed evolution sequence space depending on the composition training data. Here, we present ML-guided study enzyme investigate effects known "highly positive" (i.e., have high activity) We performed two separate series Sortase A and without highly positive called...
Inorganic material-binding proteins are valuable tools for conjugating different inorganic materials. The development of efficient methods obtaining high-affinity is desirable. In this study, focusing on ZrO2, which available in the medical field as a dental material and nanocapsule to encapsulate anticancer substances due its high biocompatibility, we first isolated peptides ZrO2BPa ZrO2BPn, bind ZrO2 nanoparticles using phage display technique. These insoluble alone. We prepared variable...
The grating substrate covered with a metal layer, plasmonic chip, and bispecific antibody can play key role in the sensitive detection of marker protein an immunosensor, because provision enhanced fluorescence signal preparation sensor surface densely modified capture antibody, respectively. In this study, one tumor markers, soluble epidermal growth factor receptor (sEGFR), was selected as target to be detected. ZnO- silver-coated chip precise regularity appropriate duty ratio periodic...
Despite the advances in surface-display systems for directed evolution, variants with high affinity are not always enriched due to undesirable biases that increase target-unrelated during biopanning. Here, our goal was design a library containing improved from information of "weakly enriched" where functional were weakly enriched. Deep sequencing previous biopanning result, no antibody mimetics experimentally identified, revealed weak enrichment partly phage infection and amplification...
Abstract We synthesized a “cyborg protein,” wherein synthetic molecule partially substitutes the main peptide chain by linking 2 protein domains with oligomer. Green fluorescent (GFP) served as model for constructing cyborg proteins. prepared circularly permuted GFP (cpGFP) new termini between β10 and β11, where original N- C-termini were linked cleavable loop. The was constructed from cpGFP β11 oligo(ethylene glycol) (OEG) using maleimide–cysteine couplings, followed enzymatic cleavage of...
During the early developmental process of organisms, formation left-right laterality requires a subtle mechanism, as it is associated with other principal body axes. Any inherent chiral feature in an egg cell can trigger this spontaneous breaking symmetry. Individual microtubules, major cytoskeletal filaments, are known objects. However, to date there lacks convincing evidence hierarchical connection molecular nature microtubules large-scale chirality, particularly at length scale entire...
Expression of secreted recombinant proteins burdens the protein secretion machinery, limiting production. Here, we describe an approach to improving production by non-conventional yeast Komagataella phaffii comprised genome-wide screening for effective gene disruptions, combining them in a single strain, and recovering growth reduction adaptive evolution. For screen, designed multiwell-formatted, streamlined workflow high-throughput assay single-chain small antibody, which is cumbersome...
We describe here the construction of artificial cellulosomes by nanoclustering recombinant cellulolytic modules on non-cellulosome-derived scaffolds. Catalytic and cellulose-binding domain derived from were assembled streptavidin inorganic nanoparticles. Heteroclustering significantly promoted activity catalytic for degradation water-insoluble substrates.
As a complementary tool to nanofluidics, biomolecular-based transport is envisioned for nanotechnological devices. We report new method guiding microtubule shuttles on multi-walled carbon nanotube tracks, aligned by dielectrophoresis functionalized surface. In the absence of electric field and in fluid flow, alignment maintained. The directed translocation kinesin propelled microtubules has been investigated using fluorescence microscopy. To our knowledge, this first demonstration gliding...
Small bispecific antibodies that induce T-cell-mediated cytotoxicity have the potential to damage late-stage tumor masses a clinically relevant degree, but their is critically dependent on structural and functional properties. Here, we constructed an optimized procedure for identifying highly cytotoxic from variety of T-cell-recruiting engineered series against cancer antigens epidermal growth factor receptor family T-cell receptors. By developing applying set rapid operations expression...
Cellulosomes, which are assemblies of cellulases with various catalytic functions on a giant scaffoldin protein carbohydrate-binding module (CBM), efficiently degrade solid cellulosic biomass by means synergistically coupled hydrolysis reactions. In this study, we constructed hybrid nanocellulosomes from the biotinylated domains (CDs) two catalytically divergent (an endoglucanase and processive endoglucanase) CBMs clustering modules streptavidin-conjugated nanoparticles. Nanocellulosomes...
Thermal decomposition of silicon oxide layers (5–20 nm in thickness) on substrates has been investigated by scanning electron microscopy and atomic force microscopy. The obtained microscopic images reveal that atoms necessary for the are supplied to thick layer at sidewall voids, which formed inhomogeneous decomposition. We also show shape voids depends surface orientation substrates, nanostructures inside owing annealing/cooling cycles. detailed observation indicates one triggers initial...
We report the observation of kinesin driven quantum dots (QDs) trapped in a microtubule loop, allowing investigation moving QDs for long time and an unprecedented distance. The QD conjugates did not depart from our observational field view, enabling tracking specific more than 5 min. unusually run length periodicity caused by loop track allow comparing studying trajectory 2 full laps, i.e., about 70 μm, statistical analysis interactions same object with obstacle. trajectories were extracted...
Production of various combinations bispecific variable domain heavy chain chain-only antibody (VHH) constructs to evaluate their therapeutic potential usually requires several gene-engineering steps. Here, we present an alternative method creating VHH in vivo through protein trans-splicing (PTS) reaction; this may reduce the number gene manipulation steps required. As a proof-of-concept, constructed (bsAb) containing anti-epidermal growth factor receptor and anti-green fluorescent VHH,...