A5100684508- Plant Pathogenic Bacteria Studies
- Plant-Microbe Interactions and Immunity
- Legume Nitrogen Fixing Symbiosis
- Genomics and Phylogenetic Studies
- Silkworms and Sericulture Research
- Insect Resistance and Genetics
- Plant Disease Resistance and Genetics
- Animal Genetics and Reproduction
- Bacterial biofilms and quorum sensing
- Plant Pathogens and Fungal Diseases
- Microbial Natural Products and Biosynthesis
- RNA regulation and disease
- Phytoplasmas and Hemiptera pathogens
- Antibiotic Resistance in Bacteria
- Plant Virus Research Studies
- Enterobacteriaceae and Cronobacter Research
- Nitrogen and Sulfur Effects on Brassica
- Nematode management and characterization studies
- Microbial Metabolic Engineering and Bioproduction
- Burkholderia infections and melioidosis
- Bacteriophages and microbial interactions
- Cystic Fibrosis Research Advances
- Bacterial Genetics and Biotechnology
- Fungal and yeast genetics research
- Agricultural pest management studies
Shanghai Jiao Tong University
2018-2025
Zhejiang Chinese Medical University
2024
Zhejiang Sci-Tech University
2016-2019
Zhejiang University
2009-2018
State Key Laboratory of Diagnosis and Treatment of Infectious Diseases
2018
Institute of Plant Protection
2018
VIB-KU Leuven Center for Cancer Biology
2015
State Key Laboratory of Rice Biology
2009-2013
China National Rice Research Institute
2012-2013
MRC Laboratory of Molecular Biology
2010-2011
Xanthomonas oryzae pv. (Xoo), the causal agent of bacterial blight rice, employs transcription activator-like effectors (TALEs) to induce expression OsSWEET family putative sugar transporter genes, which function in conferring disease susceptibility (S) rice plants. To engineer broad-spectrum resistance, we used CRISPR/Cas9-mediated gene editing disrupt TALE-binding elements (EBEs) two S OsSWEET11 and OsSWEET14, cv. Kitaake, harbors recessive resistance allele Xa25/OsSWEET13. The engineered...
Tolerance to high levels of ethanol is an ecologically and industrially relevant phenotype microbes, but the molecular mechanisms underlying this complex trait remain largely unknown. Here, we use long-term experimental evolution isogenic yeast populations different initial ploidy study adaptation increasing ethanol. Whole-genome sequencing more than 30 evolved over 100 adapted clones isolated throughout two-year experiment revealed how a interplay de novo single nucleotide mutations, copy...
Bacterial panicle blight caused by Burkholderia glumae is one of the most severe seed-borne bacterial diseases rice in world. Currently, this disease has affected many countries Asia, Africa, South and North America. It a typical example shifting from minor plant to major due changes environmental conditions. Some virulent factors B. have been identified, including toxoflavins lipases, whose productions are dependent on TofI/TofR quorum-sensing system, type III effectors. In spite its...
Stenotrophomonas maltophilia is an endophyte which plays important roles in agricultural production as a plant growth-promoting bacterium. Here, we present the draft genome sequence of strain RR-10, was isolated from rice root field China.
Soil heavy metal contamination and salinity constitute a major environmental problem worldwide. The affected area impact of these problems are increasing day by day; therefore, it is imperative to restore their potential using environmentally friendly technology. Plant growth-promoting rhizobacteria (PGPR) provides better option in this context. Thirty-seven bacteria were isolated from the rhizosphere maize cultivated metal- salt-affected soils. Some selected bacterial strains grew well...
The delivery of noncoding small RNAs (sRNAs) packaged in bacterial outer membrane vesicles (OMVs) can function as novel mediators interspecies communication. Although the role bacteria sRNAs enhancing virulence is well established, potential molecules interaction between phytopathogenic OMVs and their host plants remains unclear. In this study, we employed RNA-Seq to characterize differentially pathogen Xanthomonas oryzae pv. oryzicola (Xoc) OMVs. Our candidate sRNA (Xosr001), was found be...
Xanthomonas fragariae poses a significant emerging threat to global strawberry production. The success of surveillance strategies and quarantine measures in controlling its international spread depends heavily on the availability rapid reliable in-planta detection tools. Polymerase chain reaction (PCR) is preferred method for detecting systemic infections due high sensitivity, specificity, ease use. However, despite several PCR real-time quantitative methods, many face issues with analytical...
Two isolates of mulberry-pathogenic bacteria isolated from diseased mulberry roots were investigated in a polyphasic taxonomic study. Comparative 16S rRNA gene sequence analysis combined with rpoB allocated strains R18-2 T and R3-3 to the genus Enterobacter , asburiae E. amnigenus cancerogenus cloacae subsp. dissolvens nimipressuralis as their closest relatives. Cells Gram-negative, facultatively anaerobic rods, 0.3–1.0 µm wide 0.8–2.0 long, peritrichous flagella, showing DNA G+C content...
Abstract Background The domesticated silkworm, Bombyx mori , is the model insect for order Lepidoptera, has economically important values, and gained some representative behavioral characteristics compared to its wild ancestor. genome of B. been fully sequenced while function analysis BmChi-h BmSuc1 genes revealed that horizontal gene transfer (HGT) maybe bestow a clear selective advantage . However, role HGT in evolutionary history largely unexplored. In this study, we compare whole with...
Five nitrogen-fixing bacterial strains (SP1 T , NN143, NN144, NN208 and HX148) were isolated from stem, root or rhizosphere soil of sugar cane ( Saccharum officinarum L.) plants. Cells Gram-negative, motile, rods with peritrichous flagella. DNA G+C content was 55.0±0.5 mol%. Sequence determinations phylogenetic analysis 16S rRNA gene rpoB indicated that the affiliated genus Enterobacter most closely related to E. radicincitans DSM 16656 oryzae LMG 24251 . Fluorimetric determination thermal...
Abstract Enterobacter cloacae complex (Ecc) species are widely distributed opportunistic pathogens mainly associated with humans and plants. In this study, the genomes of clinical isolates including E. hormaechei, kobei , ludwigii non-clinical isolate nimipressuralis were analysed in combination genome asburiae by using reference strain subsp. ATCC 13047; Ecc strains tested on artificial sputum media (ASM), which mimics host, to evaluate T6SS genes as a case study. All five sequenced our...
Adenosine-to-inosine (A-to-I) RNA editing is an important posttranscriptional event in eukaryotes; however, many features remain largely unexplored prokaryotes. This study focuses on a serine-to-proline recoding (S128P) that originated the mRNA of fliC, which encodes flagellar filament protein; was observed RNA-seq samples exposed to oxidative stress. Using Sanger sequencing, we show S128P induced by H2O2. To investigate vivo interaction between RNAs and TadA, principal enzyme for A-to-I...
Abstract Plant pathogenic bacteria engage in an ongoing struggle for survival. Gram negative phytopathogenic prokaryotes deploy type III secretion system to deliver effector proteins into plant cells manipulate the host cellular environment. In many cases, targets of these are unknown. The bacterial blight pathogen, Xanthomonas oryzae pv. ( Xoo ), causes a devastating disease rice and -rice interaction is example evolutionary battle between plants pathogens. this study, we show that XopL...
Ilyonectria mors-panacis is the cause of a serious disease hampering production Panax notoginseng, an important Chinese medicinal herb, widely used for its anti-inflammatory, antifatigue, hepato-protective, and coronary heart prevention effects. Here, we report first Illumina-Pacbio hybrid sequenced draft genome assembly I. strain G3B annotation. The availability this sequence not only represents tool toward understanding genetics behind infection mechanism but also will help illuminate...
Xanthomonas translucens pv. cerealis (Xtc) causes bacterial leaf streak (BLS) of important cereal crops, including wheat (Triticum aestivum) and barley (Hordeum vulgare). Transcription activator-like effectors (TALEs) play vital roles in many plant diseases caused by spp., however, TALEs have not been previously characterized Xtc. In this study, the whole genome NXtc01, a virulent strain Xtc from Xinjiang, China, was sequenced compared with genomes other spp. NXtc01 consists single 4,622,298...
Xanthomonas oryzae pv. (Xoo), the causal agent of bacterial leaf blight in rice, delivers transcription activator-like effector (TALE) proteins into host cells to activate susceptibility or resistance (R) genes that promote disease immunity, respectively. Nonhost plants serve as potential reservoirs R genes; consequently, nonhost may trap TALEs trigger an immune response. In this study, we screened 17 Xoo for their ability induce a hypersensitive response (HR) plant Nicotiana benthamiana...
An aerobic methanotroph was isolated from a secondary sedimentation tank of wastewater treatment plant and designated strain OY6 T . Cells were Gram-stain-negative, pink-pigmented, motile rods contained an intracytoplasmic membrane structure typical type I methanotrophs. could grow at pH range 4.5–7.5 (optimum 6.5) temperatures ranging 20 °C to 37 30 °C). The major cellular fatty acids C 14 : 0 , 16 1 ω7 c /C ω6 ω5 ; the predominant respiratory quinone MQ-8. genome size 5.41 Mbp with DNA G+C...
A comparative analysis of 60 complete Burkholderia genomes was conducted to obtain insight in the evolutionary history behind diversity and pathogenicity at species level. concatenated multiprotein phyletic pattern a dataset with clusters orthologous genes (BuCOGs) were constructed. The extent horizontal gene transfer (HGT) assessed using Markov based probabilistic method. reconstruction gains losses shows that more than half families are inferred have experienced HGT least once during their...