A5059401857- Cancer Research and Treatments
- Nanoplatforms for cancer theranostics
- Gut microbiota and health
- Bacteriophages and microbial interactions
- Probiotics and Fermented Foods
- Immunotherapy and Immune Responses
- Enhanced Recovery After Surgery
- Advanced Drug Delivery Systems
- 3D Printing in Biomedical Research
- Antimicrobial Peptides and Activities
- Plant Pathogenic Bacteria Studies
- Cancer, Stress, Anesthesia, and Immune Response
- Bacterial Genetics and Biotechnology
- Plant-Microbe Interactions and Immunity
- Bacterial Infections and Vaccines
- Photodynamic Therapy Research Studies
- Methane Hydrates and Related Phenomena
- Vagus Nerve Stimulation Research
- Graphene and Nanomaterials Applications
- Phagocytosis and Immune Regulation
- IL-33, ST2, and ILC Pathways
- Microbial metabolism and enzyme function
- Tryptophan and brain disorders
- Cardiac, Anesthesia and Surgical Outcomes
- Silk-based biomaterials and applications
Renji Hospital
2021-2025
Shanghai Jiao Tong University
2021-2025
Shanghai Cancer Institute
2021-2025
State Key Laboratory of Oncogene and Related Genes
2021-2023
Wenzhou City People's Hospital
2023
Sanya Central Hospital
2023
Zhejiang Chinese Medical University
2022
Tongji University
2016-2021
LMU Klinikum
2021
Ludwig-Maximilians-Universität München
2017-2021
Abstract Surface decoration of living cells by exogenous substances offers a unique tool for understanding and tuning cell behaviors, which plays critical role in cell‐based therapy. Here, facile yet versatile approach decorating individual with multimodal coatings is reported. By simply co‐depositing dopamine under cytocompatible condition, various functional small molecules polymers can be encoded to form multifunctional coating on cell's surface. The accessibility versatility this method...
Methods capable of maintaining gut microbiota homeostasis to prevent bacterial translocation and infection under external threats are critical for multiple facets human health but have been rarely reported. Here, we describe the elicitation mucosal immunity modulate by oral delivery living probiotics into Peyer's patches. Probiotics individually camouflaged within a yeast membrane, on which embedded β-glucan can facilitate phagocytosis microfold cells that locate in intestinal epithelium....
Methods capable of distributing antitumor therapeutics uniformly and durably throughout an entire tumor would be great significance in maximizing their treatment efficacy, but they have proven to extremely challenging. Here, bacteria-mediated spatiotemporally controllable distribution combination solid tumors is reported reprogram the immune microenvironment for optimizing efficacy. By combining synthetic biology interfacial chemistry, bacteria are inside outside concurrently modified...
Outer membrane vesicles (OMVs) play vital roles in bacterial communication both intraspecifically and interspecifically. However, extracellular mechanisms of gut microbiota-derived OMVs the intestine remain poorly understood. Here, we report that released from Akkermansia muciniphila are able to (i) restore disturbed balance microbiota by selectively promoting proliferation beneficial bacteria through fusion, (ii) elicit mucosal immunoglobulin A response translocating into Peyer's patches...
An approach of decorating bacteria with triple immune nanoactivators is reported to develop tumor-resident living immunotherapeutics. Under cytocompatible conditions, tumor-specific antigens and checkpoint blocking antibodies are simultaneously conjugated onto bacterial surface then polydopamine nanoparticles formed via in situ dopamine polymerization. In addition serving as a linker, its photothermal effect can repolarize tumor-associated macrophages pro-inflammatory phenotype. The linked...
Photosensitizer-based therapy often suffers from unitary and easily attenuated photosensitive effects, limited tumor penetration retention, requirement of multiple irradiation for combination therapy, which largely restrict its application. Here, bacteria are integrated with a monochromatic irradiation-mediated ternary photosensitizers photoacoustic imaging-guided synergistic photothermal therapy. Bacteria that bioengineered to express natural melanin decorated dual synthetic by...
Abstract Disorders in the gut microbiota have been implicated various diseases, such as inflammatory bowel diabetes, and cancers. Oral microecologics are of great importance due to their ability directly intervene microbiome well noninvasiveness low side effects, while suffered from bioavailability a single therapeutic effect. Here, probiotics coated with nanocoating for synergistically enhanced biotherapy, method inspired by robust protective effectiveness silkworm cocoon. With its...
Despite immunosuppression is critical for reducing immune overactivation, existing immunosuppressive agents are largely restricted by low inhibition efficiencies and unpredictable off-target toxicities. Here, the use of dopaminergic system reported to suppress hyperactive responses in local inflamed tissues. A polydopamine nanoparticular immunosuppressant (PDNI) synthesized stimulate regulatory T (Treg) cells directly inhibit helper 1 (Th1), Th2, Th17 cells. Moreover, PDNI can activation...
Abstract Methods capable of manipulating bacterial colonization are great significance for modulating host-microbiota relationships. Here, we describe a strategy in-situ chemical reaction-mediated covalent localization bacteria. Through simple one-step imidoester reaction, primary amino groups on surface can be converted to free thiols under cytocompatible conditions. Surface thiolation is applicable modify diverse strains and the number introduced per bacterium easily tuned by varying feed...
Chirality is ubiquitous in biological systems, which closely related to functions, life processes, and even the pathogenesis of diseases. However, interface between chirality synthetic materials organisms, particularly immune system, remains poorly understood. Here, supramolecular chiral polymer micelles (SCPMs) are prepared by complexing antigenic proteins with amino acid-modified polyethyleneimine. The introduction not only reduces toxicity cationic polymer, but also benefits cell uptake...
Outer membrane vesicles (OMVs) are crucial for bacterial intercellular communication and the crosstalk between gut microbiota its host. Methods capable of visualizing derived OMVs would be great significance but have been rarely reported. Here, nanoprobes carrying a fluorescence-activating absorption-shifting tag prepared by combining genetic engineering antibiotic-boosted vesicle formation release. Benefiting from their natural structure molecular oxygen-independent emission, resulting...
Ulcerative colitis (UC) is a complex disease that results from dysregulated immune response in the gastrointestinal tract. A mouse model orally administered with dextran sodium sulfate (DSS) most widely used experimental animal of UC. However, ultrastructure colon poorly understood. In present study, colonic specimens DSS‑induced UC mice underwent hematoxylin and eosin staining, Masson's trichrome staining Verhoeff's elastic staining. addition, samples was examined by transmission electron...
Transplantation of beneficial bacteria to specific microbiota has been widely exploited treat diseases by reshaping a healthy microbial structure. However, direct exposure exogenous in vivo suffers from low bioavailability and infection risk. Here, we describe noncontact transplantation system (NMTS) core-shell microgel-enabled nonleakage envelopment. Bacteria are encapsulated into the core microgels via two-step light-initiated emulsion polymerization gelatin methacrylate. NMTS is versatile...
Abstract Bacteria‐based vaccines have received increasing attention given the ability to induce strong systemic immune responses. However, application of bacteria as therapeutic agents inevitably suffers from infection‐associated side effects due living characteristics. Here, use bacteria‐derived flagella is described construct self‐adjuvated nanofiber vaccines. With help charge‐reversal mediated by decoration with cationic polymers, can be coated negatively charged antigens through...
Xanthomonas fragariae poses a significant emerging threat to global strawberry production. The success of surveillance strategies and quarantine measures in controlling its international spread depends heavily on the availability rapid reliable in-planta detection tools. Polymerase chain reaction (PCR) is preferred method for detecting systemic infections due high sensitivity, specificity, ease use. However, despite several PCR real-time quantitative methods, many face issues with analytical...
Circular RNAs (circRNAs) are prevalent in eukaryotic cells and have been linked to disease progressions. Their unique circular structure stability make them potential biomarkers therapeutic targets. Compared with animal models, plant circRNA research is still its infancy. The lack of effective tools specifically knock down circRNAs without affecting host gene expression has slowed the progress research. Here, we developed a CRISPR-Cas13d tool that can systems, successfully achieving targeted...