A5080733615- Transgenic Plants and Applications
- Plant tissue culture and regeneration
- Viral gastroenteritis research and epidemiology
- Toxin Mechanisms and Immunotoxins
- Bioenergy crop production and management
- Biofuel production and bioconversion
- CRISPR and Genetic Engineering
- Hepatitis B Virus Studies
- Agriculture, Plant Science, Crop Management
- Plant Genetic and Mutation Studies
- Monoclonal and Polyclonal Antibodies Research
- Plant Virus Research Studies
- Wheat and Barley Genetics and Pathology
- Plant Pathogens and Resistance
- Immunodeficiency and Autoimmune Disorders
- Plant nutrient uptake and metabolism
- Seed Germination and Physiology
- Legume Nitrogen Fixing Symbiosis
- Protein purification and stability
- Agricultural economics and policies
- Herbal Medicine Research Studies
- Genetic and Environmental Crop Studies
- Blood disorders and treatments
- Viral Infectious Diseases and Gene Expression in Insects
- Supercapacitor Materials and Fabrication
Polish Academy of Sciences
2015-2025
Institute of Plant Genetics, Polish Academy of Sciences
2015-2025
Plant Breeding and Acclimatization Institute - National Research Institute
2018
Adam Mickiewicz University in Poznań
2018
Max Planck Institute for Biology
2018
Warsaw University of Life Sciences
2018
Institute of Bioorganic Chemistry, Polish Academy of Sciences
1999-2002
The infectious hepatitis B virus represents 42 nm spherical double-shelled particles. However, analysis of blood from carriers revealed the presence smaller 22 particles consisting a viral envelope surface protein. These are highly immunogenic and have been used in design vaccine produced yeast. Upon expression yeast, these proteins form virus-like that for parenteral immunization. Therefore, DNA fragment encoding antigen was introduced into Agrobacterium tumerifacience LBA4404 to obtain...
Efficient immunization against hepatitis B virus (HBV) and other pathogens with plant-based oral vaccines requires appropriate plant expressors the optimization of vaccine compositions administration protocols. Previous studies were mainly based on a combination injection small surface antigen HBV (S-HBsAg) feeding raw tissue containing antigen, supplemented an adjuvant, coming from plants conferring resistance to kanamycin. The objective this study was develop prototype formula suitable for...
Quantitative trait loci for yield, yield components and seed protein content were investigated on the basis of experiments performed with two populations pea (Pisum sativum L.) lines derived from linked crosses between Wt11238, Wt3557 Wt10245 contrasting characteristics. The yield-related traits defined as giving grain in a multiplicative way. aim was to clarify genetic architecture relation its content, possible inclusion role epistasis this explanation. To take full advantage availability...
The increasing cultivation of perennial C4 grass known as Miscanthus spp . for biomass production holds promise a sustainable source renewable energy. Unlike the sterile triploid hybrid M × giganteus , which cannot reproduce through seeds, sinensis possesses attributes that could potentially address these limitations by effectively establishing itself seed propagation. This study aimed to investigate how 18 genotypes respond chilling stress and subsequent recovery. Various traits were...
The aim of this study was to develop a freeze-drying protocol facilitating successful processing plant material containing the small surface antigen hepatitis B virus (S-HBsAg) while preserving its VLP structure and immunogenicity. Freeze-drying in lettuce leaf tissue, without any isolation or purification step, investigated. Each process step consecutively evaluated best parameters were applied. Several drying profiles excipients tested. profile 20°C for 20 h primary 22°C 2 secondary as...
Lignocellulosic biomass, including that of energy crops, can be an alternative source to produce activated carbons (ACs). Miscanthus and switchgrass straw were used ACs in a two-step process. Crushed plant material was carbonized at 600 °C then obtained carbon using NaOH or KOH 750 °C. The content surface oxygen groups determined Boehm’s method. porosity assayed the nitrogen adsorption/desorption technique, while their thermal resistance thermogravimetric derived from miscanthus...
Double haploids (DH), obtained during androgenesis in vitro or by genome diploidisation regenerated haploids, are one type of basic materials used triticale breeding programmes. The aim this study was to improve DH production a combination colchicine treatment methods on sample five winter and spring hybrids. Colchicine applied either the C17 medium induce embryo-like structures (ELS) 190-2 for green plant (GP) development. Regenerants which remained haploid were immersed solution when...
Current immunisation programmes against hepatitis B virus (HBV) increasingly often involve novel tri-component vaccines containing—together with the small (S-HBsAg)—also medium and large surface antigens of HBV (M- L-HBsAg). Plants producing all HBsAg proteins can be a source components for potential oral 'triple' anti-HBV vaccine. The objective presented research was to study M/L-HBsAg expression in leaf tissue conditions its processing prototype Tobacco lettuce carrying M- or L-HBsAg genes...
Micropropagation protocol of transgenic lettuce bearing S-, M- and L-HBsAg was developed for increased production uniformised material oral vaccine preparation. Effective manufacturing plant-based biopharmaceuticals, including vaccines, depends on sufficient content a protein interest in the initial its efficient conversion into an administrable formulation. However, stable plants with antigen is equally important reproducible processing. This can be provided by micropropagation techniques....
Chronic hepatitis B (CHB) is the cause of severe liver damage, cirrhosis, and hepatocellular carcinoma for over 240 million people worldwide. Nowadays, several types treatment are being investigated, including immunotherapy using core antigen (HBcAg) assembled into highly immunogenic capsid-like particles (CLPs). Immunogenicity plant-produced purified HBcAg, administered parenterally or intranasally, was previously reported. In this study, a novel parenteral-oral vaccination scheme proposed...
Abiotic stresses occur more often in combination than alone under regular field conditions limiting severe way crop production. Stress recognition plants primarily occurs the plasma membrane, modification of which is necessary to maintain homeostasis response it. It known that lipid transport proteins (ns-LTPs) participate lipidome cell membranes. Representative this group, ns-LTP2.8, may be involved reaction abiotic stress germinating barley by mediating intracellular hydrophobic particles,...
Core-virus like particles (VLPs) assembly is a kinetically complex cascade of interactions between viral proteins, nanoparticle’s surface and an ionic environment. Despite many in silico simulations regarding this process, there still lack experimental data. The main goal study was to investigate the capsid protein hepatitis B virus (HBc) into virus-like with superparamagnetic iron oxide nanoparticles (SPIONs) as magnetic core relation their characteristics. native form HBc obtained via...