A5074823325- Bone Tissue Engineering Materials
- 3D Printing in Biomedical Research
- Bacteriophages and microbial interactions
- Osteoarthritis Treatment and Mechanisms
- Monoclonal and Polyclonal Antibodies Research
- Characterization and Applications of Magnetic Nanoparticles
- Plant Virus Research Studies
- Additive Manufacturing and 3D Printing Technologies
- Nanoparticle-Based Drug Delivery
- Protein purification and stability
- SARS-CoV-2 and COVID-19 Research
- Olfactory and Sensory Function Studies
- Transgenic Plants and Applications
- Carbon Nanotubes in Composites
- Knee injuries and reconstruction techniques
- Tissue Engineering and Regenerative Medicine
- Graphene and Nanomaterials Applications
- Toxin Mechanisms and Immunotoxins
- Iron oxide chemistry and applications
- Total Knee Arthroplasty Outcomes
- Laser-Ablation Synthesis of Nanoparticles
- Magnetic Properties and Synthesis of Ferrites
- Protease and Inhibitor Mechanisms
- Silk-based biomaterials and applications
- Environmental Science and Water Management
Adam Mickiewicz University in Poznań
2016-2025
BOKU University
2012-2022
Institute of Molecular Biotechnology
2022
Hungarian University of Sports Science
2022
Institute of Bioorganic Chemistry, Polish Academy of Sciences
2016-2018
Poznań University of Technology
2018
Myocardial infarction (MI) is one of the most frequent causes death in industrialized countries. Stem cells therapy seems to be very promising for regenerative medicine. Skeletal myoblasts transplantation into postinfarction scar has been shown effective failing heart but shows limitations such, e.g. cell retention and survival. We synthesized investigated superparamagnetic iron oxide nanoparticles (SPIONs) as an agent direct labeling, which can used stem imaging. High quality, monodisperse...
Abstract Articular cartilage and meniscus injuries are prevalent disorders with insufficient regeneration responses offered by available treatment methods. In this regard, 3D bioprinting has emerged as one of the most promising new technologies, offering novel options. Additionally, latest achievements from fields biomaterials tissue engineering research identified constituents facilitating creation biocompatible scaffolds. study, we looked closer at hyaluronic acid multi-walled carbon...
The functional outcomes of arthroscopic matrix-based meniscus repair (AMMR) in patients two and five years after the treatment clearly show that use collagen matrix bone marrow aspirate creates favorable biological conditions for healing. This study not only provides ten follow-up results but also investigates biomolecular mechanisms governing regenerative process.Case series was based on data collected from who underwent AMMR procedure, starting with preoperatively through two-year...
This study aims to present an easily scalable, cost-efficient process of dECM extraction from porcine meniscus, dedicated bioink preparation and 3D bioprinting. Due its cartilage like structure mechanical robustness, the meniscus is exceptionally demanding tissue for decellularisation ECM. Its processing poses a great difficulty renders methods previously developed soft tissues useless. A process, combining homogenisation, hydrolysis, supercritical CO2 (scCO2) lyophilisation, was meet this...
Multifunctional core/shell type nanomaterials composed of nanocrystalline, lanthanide doped fluorides and gold nanoparticles (Au NPs) were successfully prepared. The products synthesized to combine luminescence properties the core NPs, i.e., LnF3/SiO2–NH2 KLn3F10/SiO2–NH2, plasmonic activity shell Au NPs within a single nanomaterial. luminescent (10 or 150–200 nm) separated from (6–30 using an amine modified silica (thickness ≈30 nm). exhibited bright green (Tb3+) red (Eu3+) emission under...
Cartilage injuries and bone loss become increasingly prevalent in modern societies. Articular cartilage menisci have low or no capacity for self-repair none of the available treatments provide satisfactory, long-term outcomes. Additionally, despite self-regenerating capabilities tissue, mechanism may fail insufficient, creating need surgical replacement, which is restrained by natural graft accessibility. 3D bioprinting a rapidly developing technology emerging as promising remedial therapy...
Bioengineered spider silk is a biomaterial that has exquisite mechanical properties, biocompatibility, and biodegradability. Iron oxide nanoparticles can be applied for the detection analysis of biomolecules, target drug delivery, as MRI contrast agents therapeutic hyperthermia-based cancer treatments. In this study, we investigated three bioengineered silks, MS1, MS2 EMS2, their potential to form composite material with magnetic iron (IONPs). The presence IONPs did not impede self-assembly...
One of the most important but still poorly understood issues in protein chemistry is relationship between sequence and stability proteins. Here, we present a method for analyzing influence each individual residue on foldability an entire protein. A randomly mutated library crystallizable fragment human immunoglobulin G class 1 (IgG1-Fc) was expressed surface yeast, followed by heat incubation at 79 °C selection stable variants that bound to structurally specific ligands. High throughput...
The nanoindentation method was applied to determine the elastic modulus and hardness of knee articular cartilage. Cartilage samples from both high weight bearing (HWB) low (LWB) femoral condyles were collected patients diagnosed with osteoarthritis (OA). mean HWB cartilage 4.46 ± 4.44 MPa in comparison that LWB region (9.81 8.88 MPa, p < 0.001). Similarly, significantly lower tissue (0.317 0.397 MPa) than (0.455 0.434 When adjusted patients’ ages, age group over 70 years (p A...
Abstract Superparamagnetic iron oxide nanoparticles (SPIONs) have been investigated for wide variety of applications. Their unique properties render them highly applicable as MRI contrast agents, in magnetic hyperthermia or targeted drug delivery. SPIONs surface affect a whole array parameters such as: solubility, toxicity, stability, biodistribution etc. Therefore, progress the field functionalization is crucial further development therapeutic diagnostic agents. In this study, were...
Nowadays, nanostructures having tremendous chemical and physical properties are gaining attention in the biomedical industry. However, when they prepared through classical methods (physical chemical), often non-biocompatible toxic. Considering mentioned factors, this research, organometallic silver (OMAgNs) have been by green chemistry method using acetone, methanol, methanol-hexane-based extracts of medicinally important plant Cichorium intybus. Secondary metabolites from C. intybus can be...
1The necessity to preserve meniscal function prompts the research and development of novel treatment options, like three-dimensional (3D) bioprinting. However, bioinks for 3D bioprinting have not been extensively explored. Therefore, in this study, a bioink composed alginate, gelatin, carboxymethylated cellulose nanocrystal (CCNC) was formulated evaluated. Firstly, with varying concentrations aforementioned components were subjected rheological analysis (amplitude sweep test, temperature...
The toxicity of nanoparticles (nanotoxicity) is often associated with their interruption biological membranes. effect polymer-coated magnetic (with different Fe3O4 core sizes and polymeric coatings) on a model membrane system vesicles formed by dimyristoylphosphatidylcholine (DMPC) was studied. Selected ranging from 3 to 13 nm (in diameter) were characterised transmission electron microscopy. Samples 10% DMPC nanoparticle concentrations studied attenuated total reflectance—Fourier transform...
In this study, biomimetic virus-like particles (VLPs) composed of superparamagnetic iron oxide nanoparticles (SPIONs) and receptor binding domain (RBD) the SARS-CoV-2 virus were obtained. The bioactivity VLPs was subsequently tested, utilizing natural targets RBD protein: angiotensin-converting enzyme 2 (ACE2), toll-like 4 (TLR4), neutralizing anti-SARS-CoV-2 antibodies. conducted analyses include both in vitro cellulo methods such as dynamic light scattering (DLS), ζ-potential measurement,...
The study was designed to investigate whether serum concentrations of leptin, resistin and adiponectin in obese normal-weight patients with primary knee osteoarthritis (OA) correlate clinical radiological stages the disease percentage total body fat.Seventy-three OA, divided into groups, were clinically evaluated according Knee Society Score (KSS), radiologically assessed using Kellgren Lawrence scale. fat some anthropometric data also given. Serum measured by Elisa correlated clinical,...
At present, one of the main limitations three-dimensional (3D) bioprinting in tissue engineering stems from a scarcity biomaterials tailored for specific applications. Widely used hydrogels offer an optimal printability and suitable environment cell growth; however, they lack mechanical strength required non-soft tissues, example, cartilage, tendons, meniscus. This work investigated physicochemical, mechanical, biological characteristics 3D-printed polycaprolactone (PCL) reinforced with...
Virus-like particles (VLPs) have sparked a great interest in the field of nanobiotechnology and nanomedicine. The introduction superparamagnetic nanoparticles (SPIONs) as core, provides potential use VLPs hyperthermia therapy, MRI contrast agents magnetically-powered delivery agents. Magnetite NPs also provide significant improvement terms stability. Moreover employing viral structural proteins self-assembling units has opened new paths for targeted drug systems, vaccines design, many more....
Chronic hepatitis B (CHB) is the cause of severe liver damage, cirrhosis, and hepatocellular carcinoma for over 240 million people worldwide. Nowadays, several types treatment are being investigated, including immunotherapy using core antigen (HBcAg) assembled into highly immunogenic capsid-like particles (CLPs). Immunogenicity plant-produced purified HBcAg, administered parenterally or intranasally, was previously reported. In this study, a novel parenteral-oral vaccination scheme proposed...