A5086107877- Silk-based biomaterials and applications
- Biochemical and Structural Characterization
- RNA Interference and Gene Delivery
- Phytochemical compounds biological activities
- Protease and Inhibitor Mechanisms
- Invertebrate Immune Response Mechanisms
- Antimicrobial Peptides and Activities
- Peptidase Inhibition and Analysis
- Nanoparticle-Based Drug Delivery
Greater Poland Cancer Center
2018-2024
Poznan University of Medical Sciences
2014-2024
Bioengineered spider silk is a biomaterial that has exquisite mechanical properties, biocompatibility, and biodegradability. Iron oxide nanoparticles can be applied for the detection analysis of biomolecules, target drug delivery, as MRI contrast agents therapeutic hyperthermia-based cancer treatments. In this study, we investigated three bioengineered silks, MS1, MS2 EMS2, their potential to form composite material with magnetic iron (IONPs). The presence IONPs did not impede self-assembly...
Abstract Background The optimal drug delivery system should be biocompatible, biodegradable, and allow the sustained release of only after it reaches target cells. Silk, as a natural polymer, is great candidate for building carriers. Genetically engineered silks offer possibility functionalization. Previously, we characterized bioengineered silk spheres that were functionalized with H2.1 peptide selectively delivered to Her2-positive cancer However, leakage from showed need improved control....
Aim: Analysis of the properties and chemotherapeutics delivery potential spheres made bioengineered spider silks MS1 MS2. Materials & methods: MS2 derived from Nephila clavipes dragline – MaSp1 MaSp2, respectively formed that were compared in terms physicochemical properties, cytotoxicity loading/release chemotherapeutics. Results: more dispersed, smaller, solid core, higher beta-sheet structure content, opposite (negative) charge than spheres. Preloaded showed greater applicability for...
Silk is a biocompatible and biodegradable material, able to self-assemble into different morphological structures. structures may be used for many biomedical applications, including carriers drug delivery. The authors designed new bioengineered spider silk protein, EMS2, examined its property as carrier of chemotherapeutics.To obtain EMS the MS2 monomer (that was based on MaSp2 spidroin Nephila clavipes) modified by addition glutamic acid residue. Both silks were produced in an Escherichia...
Abstract Eliminating endotoxins is a common problem in the development of biotechnologically produced pharmaceuticals or biomaterials. Residual final sample may hamper properties product induce severe adverse effects. Developing an effective downstream purification protocol that ensures lack minimal endotoxin content can be challenging task. In our previous studies, we developed nanospheres from bioengineered silks. Despite their good overall biocompatibility, vivo characterization spheres...
Event Abstract Back to Functionalized spider silk spheres stabilize in serum and deliver an active CpG-siRNA immunotherapeutic Anna Kozlowska1, Maciej Smialek1, Florczak1, 2, Ewelina Dondajewska1, Kamil Kucharczyk1, Andrzej Mackiewicz1, Marcin Kortylewski3 Hanna Dams-Kozlowska1, 2 1 Poznan University of Medical Sciences, Chair Biotechnology, Poland Greater Cancer Centre, Department Diagnostics Immunology, 3 City Hope National Center, Beckman Research Institute, United States Introduction:...