A5053880841- Tissue Engineering and Regenerative Medicine
- Muscle Physiology and Disorders
- Mesenchymal stem cell research
- Electrospun Nanofibers in Biomedical Applications
- Pluripotent Stem Cells Research
- Cancer, Hypoxia, and Metabolism
- Congenital heart defects research
- Nanoparticle-Based Drug Delivery
- Cardiac Ischemia and Reperfusion
- Cardiac Fibrosis and Remodeling
- Medical Imaging Techniques and Applications
- Mitochondrial Function and Pathology
- Bone Tissue Engineering Materials
- Cardiac Structural Anomalies and Repair
- Prostate Cancer Treatment and Research
- Hormonal and reproductive studies
- Virus-based gene therapy research
- Viral Infections and Immunology Research
- Sarcoma Diagnosis and Treatment
- Cancer Diagnosis and Treatment
- High Altitude and Hypoxia
- Cardiac Imaging and Diagnostics
- Nanoplatforms for cancer theranostics
- CRISPR and Genetic Engineering
- 3D Printing in Biomedical Research
Polish Academy of Sciences
2015-2024
Institute of Human Genetics
2014-2024
Myocardial infarction (MI) is one of the most frequent causes death in industrialized countries. Stem cells therapy seems to be very promising for regenerative medicine. Skeletal myoblasts transplantation into postinfarction scar has been shown effective failing heart but shows limitations such, e.g. cell retention and survival. We synthesized investigated superparamagnetic iron oxide nanoparticles (SPIONs) as an agent direct labeling, which can used stem imaging. High quality, monodisperse...
Ischemic heart disease, also known as coronary artery disease (CAD), poses a challenge for regenerative medicine. iPSC technology might lead to breakthrough due the possibility of directed cell differentiation delivering new powerful source human autologous cardiomyocytes. One factors supporting proper maturation is in vitro culture duration. In this study, primary skeletal muscle myoblasts were selected myogenic type reservoir genetic reprogramming. Skeletal have similar ontogeny...
Duchenne muscular dystrophy (DMD) is a genetic disorder associated with progressive deficiency of dystrophin that leads to skeletal muscle degeneration. In this study, we tested the hypothesis co-transplantation two stem/progenitor cell populations, namely bone marrow-derived mesenchymal stem cells (BM-MSCs) and muscle-derived (SM-SPCs), directly into dystrophic can improve function DMD patients. Three patients diagnosed DMD, confirmed by gene mutation, were enrolled study approved local...
In the present study, genetic modification of human skeletal muscle-derived stem/progenitor cells (SkMDS/PCs) was investigated to identify optimal protocol for myogenic cell preparation use in post-infarction heart therapy. We used two types modifications: GFP-transfection (using electroporation) and SOD3 transduction a lentiviral vector). SkMDS/PCs were cultured under different vitro conditions, including standard (21% oxygen) hypoxic (3% oxygen), latter which corresponded prevailing...
The possibility of using stem cell-derived cardiomyocytes opens a new platform for modeling cardiac cell differentiation and disease or the development drugs. Progress in this field can be accelerated by high-throughput screening (HTS) technology combined with promoter reporter system. goal study was to create evaluate responsive system that allows monitoring iPSC towards cardiomyocytes. lentiviral based on troponin 2 (TNNT2) alpha actin (ACTC) firefly luciferase mCherry, respectively....
Background: Modern therapies of post infarcted heart failure are focused on perfusion improvement the injured myocardium. This effect can be achieved by, among other means, implanting stem cells which could genetically modified with factors inducing formation new blood vessels in infarction scar area. Combined cell and gene therapy seems to a promising strategy heal an impaired The creation indirectly stimulated via vascular endothelial growth factor synthesis, for example nitric oxide...
We analyzed three cases of Complete Androgen Insensitivity Syndrome (CAIS) and report hitherto undisclosed causes the disease. RNA-Seq, Real-timePCR, Western immunoblotting, immunohistochemistry were performed with aim characterizing disease-causing variants. In case No.1, we have identified a novel androgen receptor (AR) mutation (c.840delT) within first exon in N-terminal transactivation domain. This thymine deletion resulted frameshift thus introduced premature stop codon at amino acid...
Novel contrast agents for magnetic resonance imaging based on hybrid MnO/lipids were developed and they discovered to be biocompatible efficient systems with future potential uses.
Cardiovascular diseases (CVD), with myocardial infarction (MI) being one of the crucial components, wreak havoc in developed countries. Advanced imaging technologies are required to obtain quick and widely available diagnostic data. This paper describes a multimodal approach vivo perfusion using novel SYN1 tracer based on fluorine-18 isotope. The NOD-SCID mice were injected intravenously or [18F] fluorodeoxyglucose ([18F]-FDG) radiotracers after induction MI. In all studies, positron...
Cardiovascular diseases are a growing problem in developing countries; therefore, there is an ongoing intensive search for new approaches to treat these disorders. Currently, cellular therapies focused on healing the damaged heart by implanting stem cells modified with pro-angiogenic factors. This approach ensures that introduced capable of fulfilling complex requirements environment, including replacement post-infarction scar able contract and promote formation blood vessels can supply...
Duchenne Muscular Dystrophy (DMD) is a genetic disorder characterized by disruptions in the dystrophin gene. This study aims to investigate potential therapeutic approach using genetically modified human iPS-derived mesoangioblast-like cells (HIDEMs)
Cardiovascular diseases along with MI (myocardial infarction) lead to regional ischaemia and hypoxic conditions, which prevail after infarction. Diminished O2 saturation is related elevated level of hypoxia inducible factor 1 (HIF-1) transcription factor, may switch the expression many genes. To maximize effect therapies proposed by regenerative medicine, it essential verify (within different time points MI) proangiogenic genes their receptors that are regulated, HIF-1α. We demonstrated a...
Current treatment protocols for myocardial infarction improve the outcome of disease to some extent but do not provide clue full regeneration heart tissues. An increasing body evidence has shown that transplantation cells may lead organ recovery. However, optimal stem cell population been yet identified. We would like propose a novel pro-regenerative post-infarction based on combination human skeletal myoblasts (huSkM) and mesenchymal (MSCs). huSkM native or overexpressing gene coding Cx43...
The biocompatibility of pNiPAM (Poly N-isopropylacrylamide) copolymers has been examined and they did not exert any cytotoxic effects. Their properties vulnerable temperature characteristics make them candidates for use in medical applications. We synthesized a well-characterized nanoparticles-based cargo system that would effectively deliver biological agent to human skeletal myogenic cells (SkMCs); among other aspects, downregulating apoptotic pathway potentially responsible poor...
The application of a circulating miR-195 inhibitor could be helping factor in the vitro model human skeletal muscle-derived stem/progenitor cells (SkMDS/PCs). Previously, microRNA-195 (miR-195) expression has been reported to negative for myogenesis.The study aimed obtain anti-apoptotic and anti-aging effects cultured myoblasts improve their ability form myotubes by suppressing expression.Human wild-type (WT) SkMDS/PC incubated with control (nonspecific) miRNA miR-195-inhibited SkMDS/PCs...
Abstract Although less attention was paid to understanding physical localization changes in cell nuclei recently, depicting chromatin interaction maps is a topic of high interest. Here, we focused on defining extensive organization the process skeletal myoblast differentiation. Based RNA profiling data and 3D imaging myogenic ( NCAM1, DES, MYOG, ACTN3, MYF5, MYF6, ACTN2, MYH2 ) other selected genes HPRT1, CDH15, DPP4 VCAM1 ), observed correlations between following: (1) expression change...
The aim of the study was to modify human skeletal muscle-derived stem/progenitor cells (SkMDS/PCs) and demonstrate optimal cell preparation protocol for application in post-infarction hearts. We used conditioned SkMDS/PC culture medium with α-phenyl-N-tert-butyl nitrone (PBN). SkMDS/PCs were cultured under hypoxic conditions results compared standard ones. observed a significant increase CD-56 positive phenotypic marker ability form functional myotubes, proportion young primary suspensions,...
Preclinical and clinical studies have shown that stem cells can promote the regeneration of damaged tissues, but therapeutic protocols need better quality control to confirm location number transplanted cells. This study describes in vivo imaging while assessing reporter gene expression by its binding a radiolabelled molecule respective receptor expressed target Five mice underwent human skeletal muscle-derived stem/progenitor cell (huSkMDS/PC EF1-HSV-TK) intracardial transplantation after...