A5040047607- Sperm and Testicular Function
- Reproductive Biology and Fertility
- Tissue Engineering and Regenerative Medicine
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Muscle Physiology and Disorders
- Pluripotent Stem Cells Research
- Renal and related cancers
- Mesenchymal stem cell research
- Cardiac Ischemia and Reperfusion
- Animal Genetics and Reproduction
- Electrospun Nanofibers in Biomedical Applications
- Pelvic floor disorders treatments
- Sexual Differentiation and Disorders
- CRISPR and Genetic Engineering
- Hormonal and reproductive studies
- Bone Tissue Engineering Materials
- Cardiovascular Effects of Exercise
- 3D Printing in Biomedical Research
- Reproductive System and Pregnancy
- Cardiac Fibrosis and Remodeling
- Connexins and lens biology
- Pregnancy and preeclampsia studies
- Congenital heart defects research
- Genetics and Neurodevelopmental Disorders
- Neurogenetic and Muscular Disorders Research
Institute of Human Genetics
2015-2024
Polish Academy of Sciences
2015-2024
Reduced sperm motility, defined as asthenozoospermia, is a frequent cause of male infertility, and mainly connected with the dysfunction mitochondria. The aim this study was to identify proteins, thereby metabolic pathways, responsible for using 2-DE MALDI-TOF MS, correlate results obtained those two mitochondrial tests: JC-1 MitoSox Red. test performed activity, Red check whether observed poor motility associated reactive oxygen species (ROS) production. To proteins strictly reduced men...
Myocardial infarction (MI) is one of the most frequent causes death in industrialized countries. Stem cells therapy seems to be very promising for regenerative medicine. Skeletal myoblasts transplantation into postinfarction scar has been shown effective failing heart but shows limitations such, e.g. cell retention and survival. We synthesized investigated superparamagnetic iron oxide nanoparticles (SPIONs) as an agent direct labeling, which can used stem imaging. High quality, monodisperse...
Ischemic heart disease, also known as coronary artery disease (CAD), poses a challenge for regenerative medicine. iPSC technology might lead to breakthrough due the possibility of directed cell differentiation delivering new powerful source human autologous cardiomyocytes. One factors supporting proper maturation is in vitro culture duration. In this study, primary skeletal muscle myoblasts were selected myogenic type reservoir genetic reprogramming. Skeletal have similar ontogeny...
Genetic causes that lead to spermatogenetic failure in patients with nonobstructive azoospermia (NOA) have not been yet completely established.
The aim of this study is to present results the implantation autologous myoblasts into external anal sphincter (EAS) in ten patients with fecal incontinence.After anatomical and functional assessment patients' EAS, a vastus lateralis muscle open biopsy was performed. Stem cells were extracted from specimens cultured vitro. Cell suspensions then administered EAS. Patients scheduled for follow-up visits 6-week intervals. Total 12 months.All cell procedures performed without complications. Nine...
Problem The aim of this study was to examine the expression levels IL ‐1 family members, ‐6, ‐10, TNF family, SCF , and c‐ kit in infertile patients with idiopathic non‐obstructive azoospermia ( NOA ) compared men normal spermatogenesis. Method We analyzed 20 testicular biopsy samples Affymetrix Human Gene 1.0 ST microarrays (Affymetrix, Santa Clara, CA, USA). Sixteen them were obtained from various types four Results comparative analysis pathological group demonstrated a different level 1‐...
Duchenne muscular dystrophy (DMD) is a genetic disorder associated with progressive deficiency of dystrophin that leads to skeletal muscle degeneration. In this study, we tested the hypothesis co-transplantation two stem/progenitor cell populations, namely bone marrow-derived mesenchymal stem cells (BM-MSCs) and muscle-derived (SM-SPCs), directly into dystrophic can improve function DMD patients. Three patients diagnosed DMD, confirmed by gene mutation, were enrolled study approved local...
In mammals, testicular Heat shock-related 70 kDa protein 2 (HSPA2) is a chaperon strictly linked to spermatogenesis status, whereas its presence in spermatozoa ensures successful oocyte fertilization. However, there little information on this seminal plasma infertile males. Based our previous two independent studies, we have selected HSPA2 evaluate potential biomarker of correct spermatogenesis. Using immunoblotting and mass spectrometry (MS) screened human samples for the HSPA2. Samples...
Cell differentiation is based on a synchronised orchestra of complex pathways intrinsic and extrinsic signals that manifest in the induced expression specific transcription factors pivotal genes within nucleus. One cannot ignore epigenetic status differentiating cells, comprising not only histones DNA modifications but also spatial temporal intranuclear chromatin organisation, which an important regulator nuclear processes. In present study, we investigated architecture human primary...
Introduction: Human spermatogenesis is a highly intricate process that requires the input of thousands testis-specific genes. Defects in any them at stage can have detrimental effects on sperm production and/or viability. In particular, function many meiotic proteins encoded by germ cell specific genes critical for maturation haploid spermatids and viable spermatozoa, necessary fertilization, also extremely sensitive to even slightest change coding DNA. Methods: Here, using whole exome...
In the present study, genetic modification of human skeletal muscle-derived stem/progenitor cells (SkMDS/PCs) was investigated to identify optimal protocol for myogenic cell preparation use in post-infarction heart therapy. We used two types modifications: GFP-transfection (using electroporation) and SOD3 transduction a lentiviral vector). SkMDS/PCs were cultured under different vitro conditions, including standard (21% oxygen) hypoxic (3% oxygen), latter which corresponded prevailing...
The population of healthy Polish men has not been frequently and systematically investigated for fertility status. aim this study was to assess the quality semen in a randomly recruited young males. most important task find relationship between parameters, sex hormones, AR gene polymorphism.Semen blood samples from Poznan (n=113) Lublin regions (n=89) were collected analysis, assessment hormonal concentrations, calculation CAG GGN repeats gene.Statistical comparisons hormones circulating...
The possibility of using stem cell-derived cardiomyocytes opens a new platform for modeling cardiac cell differentiation and disease or the development drugs. Progress in this field can be accelerated by high-throughput screening (HTS) technology combined with promoter reporter system. goal study was to create evaluate responsive system that allows monitoring iPSC towards cardiomyocytes. lentiviral based on troponin 2 (TNNT2) alpha actin (ACTC) firefly luciferase mCherry, respectively....
Introduction. Primary cells in vitro culture scale-up is a crucial issue cell-based tissue and organ regeneration therapy. Reducing costs space occupied by the cultured has been an important target. Cells with use of bioreactor dextran microcarriers (Cytodex ® ) have potentially chance to meet many cell therapy requirements. Material methods. We used collagen-coated carriers (Cytodex3 spinner flask develop environment suitable for human myoblast proliferation. In parallel, standard adherent...
Background: Modern therapies of post infarcted heart failure are focused on perfusion improvement the injured myocardium. This effect can be achieved by, among other means, implanting stem cells which could genetically modified with factors inducing formation new blood vessels in infarction scar area. Combined cell and gene therapy seems to a promising strategy heal an impaired The creation indirectly stimulated via vascular endothelial growth factor synthesis, for example nitric oxide...
We analyzed three cases of Complete Androgen Insensitivity Syndrome (CAIS) and report hitherto undisclosed causes the disease. RNA-Seq, Real-timePCR, Western immunoblotting, immunohistochemistry were performed with aim characterizing disease-causing variants. In case No.1, we have identified a novel androgen receptor (AR) mutation (c.840delT) within first exon in N-terminal transactivation domain. This thymine deletion resulted frameshift thus introduced premature stop codon at amino acid...
Infertility is a problem that affects approximately 15% of couples, and male infertility responsible for 40-50% these cases. The cause still poorly diagnosed treated. One the prominent causes disturbed spermatogenesis, which can lead to nonobstructive azoospermia (NOA). Whole-genome sequencing (WGS) allows us identify novel rare variants in potentially NOA-associated genes, among others, ESX1 gene. aim this study was activate gene using CRISPRa technology human germ cells (testicular...