A5085469953- Supramolecular Self-Assembly in Materials
- Polydiacetylene-based materials and applications
- Antimicrobial Peptides and Activities
- Chemical Synthesis and Analysis
- Advanced biosensing and bioanalysis techniques
- Lipid Membrane Structure and Behavior
- Surfactants and Colloidal Systems
- Calcium Carbonate Crystallization and Inhibition
- Polymer Surface Interaction Studies
- Protein Structure and Dynamics
- Hydrogels: synthesis, properties, applications
- Diatoms and Algae Research
- RNA Interference and Gene Delivery
- 3D Printing in Biomedical Research
- Molecular Sensors and Ion Detection
- Silk-based biomaterials and applications
- Minerals Flotation and Separation Techniques
- Force Microscopy Techniques and Applications
- Pickering emulsions and particle stabilization
- Petroleum Processing and Analysis
- Microbial bioremediation and biosurfactants
- Immune Response and Inflammation
- Microbial Metabolic Engineering and Bioproduction
- Luminescence and Fluorescent Materials
- DNA and Nucleic Acid Chemistry
China University of Petroleum, East China
2016-2025
China University of Petroleum, Beijing
2005-2025
Hangzhou Normal University
2025
Shandong University
2024
Ewha Womans University
2024
University of Manchester
2005-2021
Rutherford Appleton Laboratory
2021
People's Hospital of Xinjiang Uygur Autonomous Region
2021
State Council of the People's Republic of China
2019
Institute for Biotechnology and Bioengineering
2015-2017
Mixed thermoreversible gels were successfully fabricated by the addition of a thermosensitive polymer, poly(N-isopropylacrylamide) (PNIPAM), to fibrillar nanostructures self-assembled from short peptide I3K. When temperature was increased above lower critical solution PNIPAM, molecules collapsed form condensed globular particles, which acted as cross-links connect different nanofibrils and freeze their movements, resulting in formation hydrogel. Since these processes physically driven, such...
Cancer treatment requires precise tumor-specific targeting at specific sites that allows for high-resolution diagnostic imaging and long-term patient-tailorable cancer therapy; while, minimizing side effects largely arising from non-targetability. This can be realized by harnessing exogenous remote stimuli, such as tissue-penetrative ultrasound, magnetic field, light, radiation, enable local activation therapy in deep tumors. A myriad of nanomedicines efficiently activated when the energy...
Amphiphilic peptides A3K, A6K, and A9K displayed an increasing propensity for nanoaggregation with the size of hydrophobic alanine moiety, shape aggregates showed a steady transition from loose peptide stacks formed by long nanofibers to short narrow nanorods A9K. This was broadly consistent trend predicted interfacial packing curvature change if these surfactants were treated as conventional surfactants. The antibacterial capacity, defined killing percentage bacteria in given time...
CeO2 spherical crystallites aggregated by small nanoparticles in the diameter range of 5−10 nm were successfully fabricated through a facile surfactant-assisted hydrothermal method. PVP (poly(vinylpyrrolidone)) was applied as surfactant to facilitate oriented aggregation into crystallites. The size as-obtained could be deliberately controlled 100−800 varying synthetic parameters such molar ratio (repeating units) Ce(NO3)3·6H2O and concentration solution. formation mechanism briefly discussed...
Peptide and protein fibrils have attracted an enormous amount of interests due to their relevance many neurodegenerative diseases potential applications in nanotechnology. Although twisted are regarded as the key intermediate structures thick or bundles fibrils, factors determining twisting tendency handedness development from molecular supramolecular level still poorly understood. In this study, we designed three pairs enantiomeric short amphiphilic peptides: LI3LK DI3DK, LI3DK DI3LK,...
The interplay between hydrogen bonding, hydrophobic interaction and the molecular geometry of amino acid side-chains is crucial to development nanostructures short peptide amphiphiles. An important step towards developing their practical use understand how different tune bonding this process leads control size shape nanostructures. In study, we have designed synthesized three sets amphiphilic peptides (I(3)K, LI(2)K L(3)K; L(3)K, L(4)K L(5)K; I(3)K, I(4)K I(5)K) investigated I L affected...
Peptide self-assembly is of direct relevance to protein science and bionanotechnology, but the underlying mechanism still poorly understood. Here, we demonstrate distinct roles noncovalent interactions their impact on nanostructural templating using carefully designed hexapeptides, I2K2I2, I4K2, KI4K. These simple variations in sequence led drastic changes final self-assembled structures. β-sheet hydrogen bonding was found favor formation one-dimensional nanostructures, such as nanofibrils...
Peptide amphiphiles readily self-assemble into a variety of nanostructures, but how molecular architectures affect the size and shape nanoaggregates formed is not well understood. From combined TEM AFM study series cationic peptide surfactants AmK (m = 3, 6, 9), we show that structural transitions (sheets, fibers/worm-like micelles, short rods) can be induced by increasing length hydrophobic region. The trend interpreted using packing theory developed to describe surfactant transitions,...
We report the characterization of self-assembly two short β-amyloid (Aβ) peptides (16−22), KLVFFAE and Ac-KLVFFAE-NH2, focusing on examining effect terminal capping. At pH 2.0, TEM AFM imaging revealed that uncapped peptide self-assembled into long, straight, unbranched nanofibrils with a diameter 3.8 ± 1.0 nm while capped one formed nanotapes width 70.0 25.0 nm. CD analysis indicated formation β-sheet structures in both aggregated systems, but characteristic peaks were less intense...
Background Biocatalytic processes often require a full recycling of biocatalysts to optimize economic benefits and minimize waste disposal. Immobilization onto particulate carriers has been widely explored as an option meet these requirements. However, surface properties affect the amount immobilized, their bioactivity stability, hampering wide applications. The aim this work is explore how immobilization lipases magnetite nanoparticles affects biocatalytic performance under carefully...
We report a new class of cationic amphiphilic peptides with short sequences, G(IIKK)nI-NH2 (n = 1–4), that can kill Gram-positive and Gram-negative bacteria as effectively several well-known antimicrobial antibiotics. In addition, some these possess potent antitumor activities against cancer cell lines. Moreover, their hemolytic human red blood cells (hRBCs) remain remarkably low even at 10-fold bactericidal minimum inhibitory concentrations (MICs). When or tumor are cocultured NIH 3T3...
Abstract Peptide self-assembly is a hierarchical process, often starting with the formation of α-helices, β-sheets or β-hairpins. However, how secondary structures undergo further assembly to form higher-order architectures remains largely unexplored. The polar zipper originally proposed by Perutz formed between neighboring β-strands poly-glutamine via their side-chain hydrogen bonding and helps stabilize sheet. By rational design short amphiphilic peptides self-assembly, here we demonstrate...
Hydrogels offer great potential for many biomedical and technological applications. For clinical uses, hydrogels that act as scaffold materials cell culture, regenerative medicine, drug delivery are required to have bactericidal properties. The amphiphilic peptide A9K2 was designed effectively inhibit bacterial growth via a mechanism of membrane permeabilization. present study demonstrated addition fetal bovine serum (FBS) or plasma amine oxidase (PAO) induced sol-gel transition in aqueous...
Gram-negative bacteria are covered by both an inner cytoplasmic membrane (IM) and outer (OM). Antimicrobial peptides (AMPs) must first permeate through the OM cell wall before attacking IM to cause leakage kill bacteria. The bacterial is asymmetric bilayer with leaflet primarily composed of lipopolysaccharides (LPSs) phospholipids (PLs). Two cationic α-helical AMPs were designed target bacteria, a full peptide G(IIKK)3I-NH2 (G3), hydrophobic lipopeptide C8-G(IIKK)2I-NH2 (C8G2, C8 denoting...
Many de novo designed amphiphilic peptides capable of self-assembly and further structural templating into hierarchical organizations such as nanofibers gels carry more than 10 amino acid residues. A curious question is now raised about the minimal size that required for initiating amphiphilically driven nanostructuring. In this work, we show ultrashort I3K L3K could readily self-assemble stable nanostructures. While formed spherical nanospheres with diameters ∼10−15 nm, self-assembled...
Conformational orientations of a mouse monoclonal antibody to the β unit human chorionic gonadotrophin (anti-β-hCG) at hydrophilic silicon oxide/water interface were investigated using atomic force microscopy (AFM) and neutron reflectivity (NR). The surface structural characterization was conducted with concentration in solution ranging from 2 50 mg·L-1 ionic strength kept 20 mM pH = 7.0. It found that adopted predominantly "flat-on" orientation, Fc two Fab fragments lying flat on surface....
Short designed peptide amphiphiles are attractive at killing bacteria and inhibiting cancer cell growth, the flexibility in their structural design offers a great potential for improving potency biocompatibility to mammalian host cells. Amino acid sequences such as G(IIKK)nI-NH2 (n≥3) have been shown be membrane lytic, but terminal amino modifications could impose huge influence on performance. We report this work how G(IIKK)3I-NH2 its α-helical structure, penetrating ability, selective...
The use of smart drug carriers to realize cancer-targeted delivery is a promising method improve the efficiency chemotherapy and reduce its side effects. A surfactant-like peptide, Nap-FFGPLGLARKRK, was elaborately designed for based on an enzyme-triggered morphological transition self-assembled nanostructures. peptide has three functional motifs: aromatic motif Nap-FF- promote self-assembly, enzyme-cleavable segment -GPLGLA- introduce enzyme sensitivity, positively charged -RKRK- balance...
The self-assembly of short peptides is a promising route to the creation smart biomaterials. To combine peptide with enzymatic catalysis, we design an amphiphilic I3QGK that can self-assemble into long nanoribbons in aqueous solution. Upon addition transglutaminase (TGase), solution undergoes distinct sol–gel transition form rigid hydrogel, which shows strong shear-thinning and immediate recovery properties. Transmission electron microscopy (TEM) atomic force (AFM) measurements indicate...