A5067779572- Salmonella and Campylobacter epidemiology
- Viral gastroenteritis research and epidemiology
- Escherichia coli research studies
- Bacteriophages and microbial interactions
- Antimicrobial Peptides and Activities
- Graphene and Nanomaterials Applications
- Electrospun Nanofibers in Biomedical Applications
- Nanoparticles: synthesis and applications
- Block Copolymer Self-Assembly
- Immune Response and Inflammation
- Bacterial biofilms and quorum sensing
- Polymer Surface Interaction Studies
- biodegradable polymer synthesis and properties
- Animal Nutrition and Physiology
- Nanoparticle-Based Drug Delivery
- Veterinary medicine and infectious diseases
- Cell death mechanisms and regulation
- Wound Healing and Treatments
- COVID-19 Clinical Research Studies
- Tissue Engineering and Regenerative Medicine
- Cancer Research and Treatments
- Silk-based biomaterials and applications
- Animal Virus Infections Studies
- Immune Cell Function and Interaction
- Thermal properties of materials
Thermo Fisher Scientific (United States)
2022
Indian Institute of Technology Bombay
2022
Post Graduate Institute of Medical Education and Research
2021
All India Institute of Medical Sciences
2021
Vardhman Mahavir Medical College & Safdarjung Hospital
2021
Bharti Hospital
2021
Beltsville Agricultural Research Center
2018-2019
Alabama State University
2015-2018
Agricultural Research Service
2018
Trinity College Dublin
2013-2016
In order to develop a novel, safe and immunogenic fowl typhoid (FT) vaccine candidate, Salmonella Gallinarum ghost with controlled expression of the bacteriophage PhiX174 lysis gene E was constructed using pMMP99 plasmid in this study. The formation tunnel loss cytoplasmic contents observed by scanning electron microscopy transmission microscopy. No viable cells were detectable 24 h after induction an increase temperature from 37 °C 42 °C. safety protective efficacy tested chickens that...
Due to increasing antibiotic resistance, the use of silver coated single walled carbon nanotubes (SWCNTs-Ag) and antimicrobial peptides (APs) is becoming popular due their properties against a wide range pathogens. However, stability various conditions toxicity in human cells are some major drawbacks APs SWCNTs-Ag, respectively. Therefore, we hypothesized that APs-functionalized SWCNTs-Ag could act synergistically. Various covalent functionalization protocols described previously involve...
Escherichia coli causes various ailments such as septicemia, enteritis, foodborne illnesses, and urinary tract infections which are of concern in the public health field due to antibiotic resistance. Silver nanoparticles (AgNP) known for their biocompatibility antibacterial activity, may prove be an alternative method treatment, especially wound dressings. In this study, we compared efficacy two polymer-coated silver either containing 10% Ag (Ag + Polymer), or 99% (AgPVP) relation plain...
Resistance of food borne pathogens such as Salmonella to existing antibiotics is grave concern. Silver coated single walled carbon nanotubes (SWCNTs-Ag) have broad-spectrum antibacterial activity and may be a good treatment alternative. However, toxicity human cells due their physico-chemical properties serious public health Although pegylation commonly used reduce metal nanoparticle toxicity, SWCNTs-Ag not been pegylated yet, the effect on anti-bacterial cell cytotoxicity remains studied....
Escherichia coli is the most predominant Gram-negative bacterial pathogen associated with neonatal meningitis. Previous studies indicated that prototypic meningitis E. (NMEC) strain RS218 (O18:K1:H7) harbors one large plasmid. Objectives of present study were to analyze complete nucleotide sequence this plasmid (pRS218) and its contribution NMEC pathogenesis using in vitro vivo models meningitis.The 114,231 bp size, belongs incompatibility group FIB/IIA (IncFIB/IIA), contains a genetic load...
To enable benchmarking of immunogenicity between candidate severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) vaccines, there is a need for standardized, validated assays. In this article, we report the design and criteria used to validate assays outcome validation serologic functional evaluation immune response antibody titers in human serum. A quantitative cell-based microneutralization (MNT) assay, utilizing reference standard, detecting anti-SARS-CoV-2 spike...
To construct a novel live vaccine candidate for fowl typhoid (FT) caused by Salmonella Gallinarum (SG), the lon and cpxR genes that are related to host-pathogen interaction were deleted from wild type SG using allelic exchange method. The mutants grown normally, as was type. biochemical properties of remained very similar those wild-type, while JOL914 (Deltalon) JOL916 (DeltalonDeltacpxR) mucoid. Extracellular polysaccharide increased 30.6-, 1.3-, 46.2-fold in JOL914, JOL915 (DeltacpxR),...
A new strategy to develop an effective vaccine is essential control food-borne Salmonella enterica serovar Enteritidis infections. Bacterial ghosts (BGs), which are nonliving, Gram-negative bacterial cell envelopes, generated by expulsion of the cytoplasmic contents from cells through controlled expression using modified cI857/λ P(R)/gene E system. In present study, pJHL99 lysis plasmid carrying mutated lambda pR37-cI857 repressor and PhiX174 gene was constructed transformed in S. produce a...
Abstract: The antimicrobial activity of silver-coated carbon nanotubes (AgCNTs) and their potential mode action against mucoid nonmucoid strains Pseudomonas aeruginosa was investigated in vitro. results showed that AgCNTs exhibited both with minimum inhibitory concentrations approximately 8 µg/mL, indicating a high sensitivity P. to AgCNTs. were also bactericidal at the same concentration. Scanning transmission electron-microscopy studies further revealed majority cells treated transformed...
Objective To investigate the toxicity and antibacterial application of antimicrobial peptide-functionalized silver-coated carbon nanotubes against Staphylococcus infection using a full thickness human three-dimensional skin model. Materials methods The formation on scaffolds was characterized by electron microscopy investigation several cell markers real time–reverse transcriptase polymerase chain reaction. Functionalized were prepared carboxylated with peptides such as TP359, TP226 TP557....
Abstract Biodegradable and biocompatible polymers that are engineered to nanostructures play a key role in providing solution for sustained chemotherapy. This study is focused on preparation, drug encapsulation efficiency, in‐vitro release, cellular uptake cell viability of poly(caprolactone) grafted dextran (PGD) nanoparticles (NPs) formulation containing vinblastine as the anticancer drug. Drug‐loaded PGD NPs were prepared by modified oil/water emulsion method characterized laser light...
Block copolymers (BCPs) are seen as a possible cost effective complementary technique to traditional lithography currently used in the semiconductor industry. This unconventional approach has received increased attention recent years process capable of facilitating ever decreasing device size demanded. Control over microdomain orientation and enhancing long range order key aspects for utility BCPs future lithographic purposes. paper provides an efficient route fabrication highly ordered...
The electronics industry is beginning to show interest in 2D molybdenum disulfide (2D‐MoS 2 ) as a potential device material due its low band gap and high mobility. However, current methods for synthesis are not “fab” friendly require harsh environments processes. Here, novel method prepare MoS nanowire arrays layered structures via self‐assembly of block copolymer system reported. Well‐controlled films microphase separated line‐space nanopatterns have been achieved by solvent annealing...