A5050573102- Enzyme-mediated dye degradation
- Plant Pathogens and Fungal Diseases
- Fungal Biology and Applications
- Biochemical and biochemical processes
- Mycorrhizal Fungi and Plant Interactions
- Biofuel production and bioconversion
- Lignin and Wood Chemistry
- Microbial Metabolism and Applications
- Forest Ecology and Biodiversity Studies
- Genomics and Phylogenetic Studies
- Enzyme Catalysis and Immobilization
- Microbial Metabolic Engineering and Bioproduction
- Plant and Fungal Interactions Research
- Lichen and fungal ecology
- Wood and Agarwood Research
- Yeasts and Rust Fungi Studies
- Enzyme Production and Characterization
University of Helsinki
2012-2021
Biocenter Finland
2016-2021
University of Turku
2020
The white-rot Agaricomycetes species Phlebia radiata is an efficient wood-decaying fungus degrading all wood components, including cellulose, hemicellulose, and lignin. We cultivated P. in solid state cultures on spruce wood, extended the experiment to 6 weeks gain more knowledge time-scale dynamics of protein expression upon growth decay. Total proteome transcriptome were analyzed by peptide LC–MS/MS RNA sequencing at specific time points study enzymatic machinery fungus' natural substrate....
Mitochondria are eukaryotic organelles supporting individual life-style via generation of proton motive force and cellular energy, indispensable metabolic pathways. As part genome sequencing the white rot Basidiomycota species Phlebia radiata, we first assembled its mitochondrial (mtDNA). So far, 156 348 bp mtDNA is second largest described for fungi, considerable size among eukaryotes. The P. radiata as single circular dsDNA molecule containing genes large small ribosomal RNAs, 28 transfer...
The fungal genus Phlebia consists of a number species that are significant in wood decay. Biotechnological potential few for enzyme production and degradation lignin pollutants has been previously studied, when most the this unknown. Therefore, we carried out wider study on biochemistry systematics species. Isolates belonging to were subjected four-gene sequence analysis order clarify their phylogenetic placement at level evolutionary relationships among phlebioid Polyporales. rRNA-encoding...
Fomitopsis pinicola is a species of Polyporales frequently encountered in Nordic temperate and boreal forests. In nature, the fungus causes destructive brown rot wood, colonizing tree trunks often occupied by other Basidiomycota species. We mimicked these species-species interactions introducing F. to five white species, all common saprotrophs Norway spruce. Hyphal mycelial growth various combinations were recorded, while activities lignocellulose-acting CAZymes oxidoreductases followed...
Summary Feruloyl esterases ( FAE s) are accessory enzymes for plant biomass degradation, which catalyse hydrolysis of carboxylic ester linkages between hydroxycinnamic acids and cell‐wall carbohydrates. They a diverse group evolved from, e.g. acetyl xylan AXE s), lipases tannases, thus complicating their classification prediction function by sequence similarity. Recently, an increasing number fungal s have been biochemically characterized, owing to potential in various biotechnological...
Summary We studied the effect of climate warming on H eterobasidion root rot in boreal forests by measuring respiration activity pure cultures parviporum Norway spruce ( P icea abies ) sawdust and linking these data to temperature obtained from three located along a north‐south transect stretching northern G ermany F inland. The applied this investigation were homokaryotic, but separate investigation, we found no significant difference between homo‐ heterokaryotic isolates. also that...
The kraft lignin's low molecular weight and too high hydroxyl content hinder its application in bio-based carbon fibers. In this study, we were able to polymerize lignin reduce the amount of groups by incubating it with white-rot fungus Obba rivulosa. Enzymatic radical oxidation reactions hypothesized induce condensation lignin, which increased aromatic rings connected carbon–carbon bonds. This modification is assumed be beneficial when aiming for graphite materials such as Furthermore,...
The white rot fungus Phlebia radiata, a type species of the genus Phlebia, is an efficient decomposer plant cell wall polysaccharides, modifier softwood and hardwood lignin, able to produce ethanol from various waste lignocellulose substrates. Thus, P. radiata promising organism for biotechnological applications aiming at sustainable utilization biomass. Here we report genome sequence isolate 79 originally isolated decayed alder wood in South Finland. To better understand evolution decay...
Summary Trees are known to have adapted local climatic conditions, but the adaptation of their pathogenic associates is poorly understood. Heterobasidion parviporum causes root and butt rot in spruce. In this work, respiration H . subpopulations from climatically diverse environments was examined at various temperatures. Isolates were obtained three areas E urope (southern F inland, D enmark northern I taly) two locations C entral S iberia ( K rasnoyarsk rkutsk). Respiration rates measured...
Laccases are multi-copper oxidases that use molecular oxygen as the electron acceptor to oxidize phenolic and indirectly also non-phenolic substrates by mechanisms involving radicals. Due their eco-friendliness broad substrate specificity, laccases span a wide range of biotechnological applications. We have heterologously expressed laccase from coprophilic basidiomycete Coprinopsis cinerea (CcLcc9) in methylotrophic yeast Pichia pastoris. The recombinant CcLcc9 (rCcLcc9) oxidized...
Summary Fungal laccases are attracting enzymes for sustainable valorization of biorefinery lignins. To improve the lignin oxidation capacity two previously characterized laccase isoenzymes from white‐rot fungus Obba rivulosa , we mutated their substrate‐binding site at T1. As a result, pH optimum recombinantly produced variant r Or Lcc2‐D206N shifted by three units towards neutral pH. O. variants with redox mediators oxidized both dimeric model compound and poplar lignin. Significant...
Background: Manganese peroxidases (MnP) and lignin (LiP) are haem-including fungal secreted class-II peroxidases, which interesting oxidoreductases in protein engineering aimed at designing of biocatalysts for lignocellulose conversion, dye compound degradation, activation aromatic compounds, biofuel production. Keywords: peroxidase, white-rot fungi, biodegradation, decolorizing, Phlebia radiata, recombinant enzyme, engineering.