Abstract In this study, a new analytical method for erdosteine (ERD) in plasma based on high‐performance liquid chromatography and fluorimetric detector, is presented. Precolumn derivatization of ERD with 4‐bromomethyl‐7‐methoxy coumarin (BrMmC) dibenzo‐18‐crown‐6‐ether as reaction catalyst led to the production fluorescent compound. was monitored by fluorescence an excitation wavelength λ ext. = 325 nm emission em. 390 nm. Optimum conditions were carefully studied optimized. A...

A sensitive method is presented for the determination of ticagrelor (TCG) in human plasma and pharmaceutical preparations using 5-(dimethylamino)naphthalene-1-sulfonyl chloride (dansyl chloride).Ticagrelor contains a secondary amino group which reacts with dansyl presence 0.5 M sodium bicarbonate (pH 10) to yield highly fluorescent derivative that measured at 445 nm after excitation 340 nm.Different experimental parameters affecting fluorescence intensities were carefully studied...

An ultra fast liquid chromatographic (UFLC) method using fluorimetric detection for the quantitation of Febuxostat (FEB) in human serum was developed by precolumn derivatization with 4-bromomethyl-7-methoxy coumarin (BrMmC). The catalyzed dibenzo-18-crown-6-ether to produce a fluorescent derivative which determined through fluorescence measurement excitation at 320 nm and emission 395 wavelengths. A C18 column 100 cm x 4.6 mm, 3 µm particule size used UFLC separation procedure. mixture...

Atomoxetine is a special pharmaceutics used for attention deficit hyperactivity disorder. It finds application in various age groups, including children, adolescents, and adults. Ultra high-performance liquid chromatographic technique (UHPLC) an exceptional provides swift, uncomplicated, highly sensitive results. Such has been formulated to analyse atomoxetine levels both breast milk human plasma. Magnetic micro solid phase extraction simple, rapid, efficient, precise method which was...

Objective: Two simple, rapid and sensitive methods were developed for determination of Erdosteine (ERD) in pure form as well their pharmaceutical formulations. Methods:The based on formation colored charge transfer complexes with ERD chloranil (CA) 7,7,8,8-tetracyanoquinodimethane (TCNQ).The obtained charge-transfer measured at 454 843 nm CA TCNQ methods, respectively.Optimization different experimental conditions investigated.Results: Beer's plots obeyed a general concentration range 10-500...

Although the first application of Quality by Design (QbD) concept started for product development studies, number studies regarding its to analytical has been increased recently. Basically, QbD strategy in both formulation and analytic are identical logically conceptually, but they have somedifferences terms terminology application. Essential approach differences this are; determination target profile, critical method characteristics, process parameters, study area. However, risk evaluation...

A novel analytical method based on ultra-fast liquid chromatography using fluorimetric detector was developed and validated for determination of non-steroidal anti-inflammatory drugs (NSAIDs) (ibuprofen (IBP), etodolac (ETD), dexketoprofen (DKP), sodium diclofenac (SDCF), naproxen (NPX) in tap water, urine pharmaceutical samples. Precolumn derivatisation targeted NSAIDs carried out with 4-bromomethyl-7-methoxy coumarin (BrMmC) dibenzo-18-crown-6-ether as reaction catalyst leading to the...

Objective: In current study, quantification of the capsaicinoids in chilli sauces based on a sensitive ultra fast liquid chromatography method and derivatization with dansyl chloride (DNS-Cl) was described.Capsaicinoids are biosynthesized as secondary metabolites by sauces.The major components capsaicin (CPS) dihydrocapsaicin (DCPS).Methods: Phenol groups within CPS DCPS suitable for reaction using DNS-Cl (chemically named 5-(dimethylamino) naphthalene-1-sulfonyl chloride) at pH 10 0.5 M...

A new spectrofluorimetric technique was produced for the analysis of benidipine (BDP) in tablets and spiked plasma samples.The developed method based on coupling between Benidipine, (a secondary amine group), 7-fluoro-4-nitrobenzo-2-oxa-1,3-diazole (NBD-F) forming fluorescent dervatived as a NBD-Benidipine.The reaction occured by using pH 8.5 buffer solution to form derivatives that are measured λem: 550 nm λex: 465 nm.A variable parameters effecting derivatization process were studied.The...

This article aims to present a sensitive, and precise stability-indicating ultra fast liquid chromatographic method, which was developed determine ticagrelor (TCG) in pharmaceutical formulations spiked plasma samples.Chromatographic separation achieved under isocratic elution by using C18 column (100 × 4mm, 3μm) mixture of acetonitrile phosphoric acid solution (adjusted pH 3.0 triethylamine) (55:45, v/v) at flow rate 0.7 mL per minute.The analyte detected wavelength 254 nm, photodiode array...

Amaç: Bu çalışma, nebivolol (NEB) ve amlodipinin (AML) kantitatif analizini yapmak için yüksek performanslı sıvı kromatografisi (HPLC) kullanarak bir yöntem geliştirmeyi validasyonunu amaçlamıştır Yöntemler: Kromatografik sistemdeki ayırma, asetat tamponu (pH: 4,5) asetonitril karışımından oluşan mobil faz kullanılarak, 1,3 mL/dakika akış hızında, C18 kolonda (150 mm x 4,6 mm, 2,6 m) gerçekleştirildi.Diyot sıralı dedektör 265 nm dalga boyuna ayarlandı.Uluslararası Uyum Konferansı...

A simple, rapid, precise and accurate isocratic reversed phase HPLC method was developed validated for the determination of lercanidipine hydrochloride in pharmaceutical tablets spiked human breast milk.The chromatographic separation achieved on C18 (250×4.6 mm×5µm) column using a mobile consisting acetonitrile phospate buffer (pH=4) (55:45, v/v) at flow rate 1.1 mL/min UV detection 237 nm.The linearity proposed investigated range 1.0-40 μg/mL (r 2 =0.9990).The terms accuracy, precision,...

Abstract A novel simple and cost effective HPLC technique was presented for the quantification of selexipag (SLP) in human plasma sample technique's applicability to a pharmacokinetic investigation. C18 (5 µm x 4.6 mm 150 mm) column used at 30°C with mobile phase consisted acetonitrile: % 0.5 formic acid (65:35 v/v) 1.2 mL min − 1 flow rate. The linearity range is 10–125 ng . As preparation step precipitated acetonitrile detection provided 300 nm. LOD found be 3.3 drug. Validation studied...

Objective: In this study, analysis of gemifloxacin in pharmaceutical preparations was performed the presence 4-bromomethyl-7methoxycoumarin reagent and dibenzo-18-crown-6 ether catalyst, by high-performance liquid chromatography. Methods:The excitation wavelength compound formed as a result derivatization process found λext.=325 nm emission λem.=390 nm.Optimum reaction conditions were carefully studied.Chromatographic separations 150 cm x4.6 mm, 5 µm I.D C18 column, mobile phase consisting...

Abstract A novel simple and cost effective HPLC technique was presented for the quantification of selexipag (SLP) in human plasma sample technique's applicability to a pharmacokinetic investigation. Chromatographic separation achieved with C18 (5 µm × 4.6 mm 150 mm) column, at 30 °C isocratic elution, mobile phase composed solution (acetonitrile), B (0.5% formic acid) (65:35 v/v) flow rate 1.2 mL min −1 . The linearity range is 10–150 ng As preparation step precipitated acetonitrile...

Meksiletin Hidroklorur akut ve kronik ventrIkuler aritmilerde kullanilan antiarit-mik etkili bir ilactir. Bu calismada, meksiletinin kapsullerde metiloranj ile Iyon cifti olu­ sumuna dayanan yeni spektrofotometrik yontem gelistirilmesi esas alinmistir. Olusan iyon ciftinin kloroformdaki maksimum absorpsiyonu 423 nm de gozlenmistir. Olcu eg­risi 1.1- 7.6 M.g.ml arasinda dogrusaldir. Gelistirilen meksiletin hidroklorur miktar tayinine uygulan­mistir. Sonuclar UV-spektrofotometrik elde edilen...