A5075923438- Wnt/β-catenin signaling in development and cancer
- Inflammatory mediators and NSAID effects
- Cellular Mechanics and Interactions
- Cardiomyopathy and Myosin Studies
- Peroxisome Proliferator-Activated Receptors
- Cancer-related Molecular Pathways
- Cancer Cells and Metastasis
- Eicosanoids and Hypertension Pharmacology
- Angiogenesis and VEGF in Cancer
- Cancer, Hypoxia, and Metabolism
- Protein Kinase Regulation and GTPase Signaling
- Nitric Oxide and Endothelin Effects
- Microtubule and mitosis dynamics
- Cell Adhesion Molecules Research
- Cardiac Fibrosis and Remodeling
- Receptor Mechanisms and Signaling
- Cancer, Stress, Anesthesia, and Immune Response
- Cardiovascular Effects of Exercise
- Protease and Inhibitor Mechanisms
- Peptidase Inhibition and Analysis
- Skin and Cellular Biology Research
- Cytokine Signaling Pathways and Interactions
- Blood Pressure and Hypertension Studies
- Ion channel regulation and function
- Ubiquitin and proteasome pathways
Kyushu University
2015-2024
University of Occupational and Environmental Health Japan
1995-2024
Osaka University
1996-2010
Chiba University
2010
University of Iowa
2010
Osaka Medical and Pharmaceutical University
2010
The University of Tokyo
2010
Fukuoka University
2003-2007
Zenyaku Kogyo (Japan)
2007
Kobe University
2007
Abstract —Alterations in the functions of vascular endothelial cells (ECs) induced by fluid shear stress may play a pivotal role both development and prevention diseases. We found that DNA synthesis bovine aortic human umbilical vein ECs, determined [ 3 H]thymidine incorporation, was inhibited steady laminar (5 30 dyne/cm 2 ). This growth inhibition due to associated with suppression cell transition from G 1 S phase cycle. Therefore, we studied -phase events find molecules responsible for...
We created knock-in mice in which a deletion of 3 base pairs coding for K210 cardiac troponin (cTn)T found familial dilated cardiomyopathy patients was introduced into endogenous genes. Membrane-permeabilized muscle fibers from mutant showed significantly lower Ca(2+) sensitivity force generation than those wild-type mice. Peak amplitude transient cardiomyocytes increased mice, and maximum isometric produced by intact not different that suggesting augmented to compensate decreased...
Nitric oxide-generating vasodilators inhibit vascular smooth muscle cell proliferation. To elucidate the mechanism underlying this process, we investigated effect of S-nitroso-N-acetylpenicillamine (SNAP), a nitric oxide-releasing agent, on cycle. When G0 cells were stimulated with fetal bovine serum and basic fibroblast growth factor, DNA synthesis assessed by [3H]thymidine incorporation started about 15 h later. SNAP dose-dependently inhibited incorporation, was maximal at 100 μM. This...
A deletion mutation ΔK210 in cardiac troponin T (cTnT) was recently found to cause familial dilated cardiomyopathy (DCM). To explore the effect of this on muscle contraction under physiological conditions, we determined Ca 2+ -activated force generation permeabilized rabbit fibers into which mutant and wild-type cTnTs were incorporated by using our TnT exchange technique. The free concentrations required for higher cTnT-exchanged than ones, with no statistically significant differences...
The activities of phosphatidylinositol 4,5-bisphosphate (PIP2) phosphodiesterase (PDE) and inositol 1,4,5,-trisphosphate (IP3) phosphatase in the particulate cytosol fractions prepared from porcine coronary artery smooth muscles were examined using 32P-labelled PIP2 IP3 as substrates, respectively. activity PDE, assessed production IP3, fraction was about 10-fold higher than that fraction. In absence MgCl2, PDE both showed no causal relation to free Ca2+ concentration physiological range...
Dilated cardiomyopathy (DCM), characterized by dilatation and dysfunction of the left ventricle, is an important cause heart failure. Many mutations in various genes, including cytoskeletal protein genes contractile have been identified DCM patients, but mechanisms how such lead to remain unknown.We established mouse model expressing a mutated cardiac alpha-actin gene, which has reported patients with DCM, (mActin-Tg). mActin-Tg mice showed gradual resulting death The number apoptotic...
Fluid shear stress induces cyclooxygenase (COX)-2 gene expression in vascular endothelial cells. We investigated the underlying mechanism of this induction.Exposure human umbilical vein cells to laminar physiological range (1 30 dyne/cm2) upregulated COX-2 but not COX-1, a constitutive isozyme COX. The mRNA began increase within 0.5 hour after loading and reached maximal level at 4 hours. Roles promoter region 3'-untranslated were evaluated by transient transfection luciferase reporter...
Ligands for peroxisome proliferator-activated receptor gamma, such as the thiazolidinedione class of antidiabetic drugs and 15-deoxy-Delta(12,14)-prostaglandin J(2) (15d-PGJ(2)), modulate various processes in atherogenesis. In search cells that generate prostaglandin D(2) (PGD(2)), metabolic precursor 15d-PGJ(2), we identified PGD(2) from culture medium endothelial cells. To study how production is regulated cells, investigated role fluid shear stress metabolism PGD(2). Endothelial expressed...
In search of chemical substances applicable for the treatment cancer and other proliferative disorders, we studied signal transduction <i>Dictyostelium</i>differentiation-inducing factors (DIFs) in mammalian cells mainly using HeLa cells. Although DIF-1 DIF-3 both strongly inhibited cell proliferation by inducing G<sub>0</sub>/G<sub>1</sub> arrest, was more effective than DIF-1. suppressed cyclin D1 expression at mRNA protein levels, whereas overexpression overrode DIF-3-induced cycle...
To elucidate the role of protein kinase C in vascular smooth muscle cell proliferation, we examined effects phorbol 12-myristate 13-acetate (PMA) on G1 events human arterial cells. About 15 h after G0 cells were stimulated with fetal bovine serum and basic fibroblast growth factor, [3H]thymidine incorporation started. PMA (10 nM) inhibited over 90% when added earlier than 3 stimulation, but had no effect 12 or later. phosphorylation retinoblastoma (pRb), which normally began at about 9 h....
1 To investigate the vasodilator actions of nitroglycerine and isoprenaline, effects these agents, dibutyryl cyclic AMP (db AMP) 8-bromo GMP (8-Br GMP) on intact muscle tissue, skinned rabbit mesenteric artery were investigated. 2 In porcine coronary artery, (>0.1 μm) increased production with no change in amount AMP, while isoprenaline significantly GMP. 3 or inhibited tonic component 39 mm [K]o-induced contraction to a greater extent than phasic component. Nitroglycerine 8-Br showed...
BACKGROUND AND PURPOSE Catechins, biologically active polyphenols in green tea, are known to have a protective effect against cardiovascular diseases. In this study, we investigated direct actions of tea catechins on cardiac muscle function explore their uses as potential drugs for disease. EXPERIMENTAL APPROACH The effects were systematically the force‐pCa relationship skinned fibres determine myofilament contractility. mechanisms action effective using troponin exchange techniques, quartz...
Background: It has not been fully elucidated whether antihypertensive medication adherence affects blood pressure (BP) control in hypertension cases. Aim: To investigate the association of to drug regimens and BP using data from Combination Pill Losartan Potassium Hydrochlorothiazide for Improvement Medication Compliance Trial (COMFORT) study. Design: An observational analysis a randomized controlled trial. Methods: A total 203 hypertensive subjects were randomly assigned daily regimen...
We previously reported that celecoxib, a selective COX-2 inhibitor, strongly inhibited human colon cancer cell proliferation by suppressing the Wnt/β-catenin signaling pathway. 2,5-Dimethylcelecoxib (DM-celecoxib), celecoxib analog does not inhibit COX-2, has also been to have an antitumor effect. In present study, we elucidated whether DM-celecoxib inhibits intestinal growth, and its underlying mechanism of action. First, compared effect with on lines HCT-116 DLD-1. suppressed T-cell factor...
Background: Patients with mild-to-moderate essential hypertension in the HOMED-BP trial were randomly allocated to first-line treatment a calcium channel blocker (CCB), angiotensin-converting enzyme inhibitor (ACEI) or angiotensin II receptor (ARB). Methods: We recruited 265 (93 for CCB, 71 ACEI and 101 ARB) patients who completed genomic study. Home blood pressure was measured 5 days off-treatment before randomization after 2–4 weeks of randomized drug treatment. Genotyping performed by...