A5051567021- Renal Transplantation Outcomes and Treatments
- Renal Diseases and Glomerulopathies
- Receptor Mechanisms and Signaling
- Renin-Angiotensin System Studies
- Pharmacological Effects and Toxicity Studies
- Organ Donation and Transplantation
- Transplantation: Methods and Outcomes
- Amyloidosis: Diagnosis, Treatment, Outcomes
- Polyomavirus and related diseases
- Hormonal Regulation and Hypertension
- Ion channel regulation and function
- Ion Transport and Channel Regulation
- Parathyroid Disorders and Treatments
- Neurological Complications and Syndromes
- Renal and Vascular Pathologies
- Nitric Oxide and Endothelin Effects
- Electrolyte and hormonal disorders
- Cancer Genomics and Diagnostics
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Dietary Effects on Health
- Ureteral procedures and complications
- SARS-CoV-2 and COVID-19 Research
- Advanced Drug Delivery Systems
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Glycogen Storage Diseases and Myoclonus
UC Davis Health System
2023
University of California Davis Medical Center
2020-2022
University of California, Davis
2018-2022
University of Michigan–Ann Arbor
1996-2017
Ann Arbor Center for Independent Living
2015
National Institutes of Health
2004-2014
Johns Hopkins Medicine
2012
Johns Hopkins University
2012
CDC Foundation
2011
Washington University in St. Louis
2008
The present study was undertaken to investigate the mRNA localization of two major kidney-specific Na-K-Cl transport proteins, bumetanide-sensitive cotransporter (NKCC2 in rabbit and BSC1 rat) thiazide-sensitive (TSC). NKCC2 from mouse has been shown exist three isoforms (designated A, B, F) that differ only a 96-bp region. divergent region each cloned rat kidney by polymerase chain reaction (PCR)-based strategy, isoform-specific primers were chosen. RNA cDNA prepared renal cortex medulla...
Niacinamide inhibits intestinal sodium/phosphorus transporters and reduces serum phosphorus in open-label studies. A prospective, randomized, double-blind, placebo-controlled crossover trial was performed for assessment of the safety efficacy niacinamide.Hemodialysis patients with levels > or =5.0 mg/dl were randomly assigned to 8 wk niacinamide placebo, titrated from 500 1500 mg/d. After a 2-wk washout period, switched alternative therapy. Vitamin D analogs calcimimetics held constant;...
To provide a frame of reference for studies renal divalent cation and phosphate metabolism, we assessed the cellular localization kidney calcium receptor (RaKCaR), parathyroid hormone-related protein (PTHrP), hormone/ (PTH/PTHrP) mRNA. The used using reverse transcription-polymerase chain reaction (RT-PCR) applied to cDNA prepared from dissected rat nephron segments primary cultures mouse juxtaglomerular granular cells. With species-specific primers, PCR products expected size were obtained...
Experiments were performed in a recently generated strain of mice with an angiotensin II AT1A-receptor null mutation (M. Ito, M. I. Oliverio, P. J. Mannon, C. F. Best, N. Maeda, O. Smithies, and T. coffman. Proc. Natl. Acad. Sci. USA 92: 3521-3525, 1995) to examine the effects chronic AT1A receptor deficiency on tubuloglomerular feeback (TGF) responses. All animals genotyped by polymerase chain reaction using primers designed amplify sequences from deleted gene neomycin resistance gene....
The attrition rate of functioning allografts beyond the first year has not improved despite immunosuppression, suggesting that nonimmune mechanisms could be involved. Notably, glomerulopathies may account for about 40% failed kidney engraftment, and glomerulosclerosis progression to ESRD are caused by podocyte depletion. Model systems demonstrate nephrectomy can precipitate hypertrophic stress triggers progressive depletion leading ESRD, this process is accompanied accelerated detachment...
Lower eGFR 1 year after kidney transplant is associated with shorter allograft and patient survival. We examined how practice changes in the past decade correlated time trends average at United States a cohort of 189,944 patients who received between 2001 2013. calculated for recipient each using appropriate Modification Diet Renal Disease equation depending on prevailing methodology creatinine measurement, used linear regression to model effects national post-transplant trend. Between...
Adjustments of renal vascular resistance in response to changes blood pressure are mediated by an interplay between the myocyte-inherent myogenic and kidney-specific tubuloglomerular feedback (TGF) mechanisms. Using mice with deletion A(1) adenosine receptor (A1AR) gene, we tested prediction that absence TGF, previously established result from A1AR deficiency, is associated a reduction efficiency autoregulation. In anesthetized wild-type (A1AR+/+) A1AR-deficient (A1AR-/-), glomerular...
The vast majority of polyomavirus nephropathy (PVN) is due to BK virus, but rare cases result from JC virus reactivation. To date, only a handful biopsy-proven JC-PVN have been reported. Here, we describe the clinical and pathologic findings in 7 patients with JC-PVN.Search pathology archives at 2 institutions found JC-PVN. Clinical data were extracted electronic medical records, biopsies reviewed.Four diagnosed 6 y posttransplant or later. remaining 3 presented within approximately...
In the present study we have investigated whether presence of a second renin gene exerts an overriding influence on plasma such that mice with two genes consistently higher levels than only one gene.Plasma was determined as rate angiotensin I generation using radioimmunoassay (RIA) kit (plasma concentration, PRC) or without activity, PRA) addition purified rat angiotensinogen substrate.In male 129SvJ, DBA/2 and Swiss Webster mice, strains possessing both Ren-1 Ren-2, PRC (ng Ang mL(-1)...
Abstract Aim: By crossing aquaporin 1 (AQP1)−/− and adenosine receptor (A1AR)−/− mice, we generated an animal model that combines a proximal tubular absorption defect with absence of tubuloglomerular feedback (TGF) regulation glomerular filtration rate (GFR). The aim studies in these animals was to determine whether TGF‐induced reduction GFR is prerequisite for preventing potentially fatal fluid losses. Methods Results: In contrast AQP1 deficient AQP1/A1AR−/− mice were found have normal GFR....
Recent studies evaluated and proposed new race-neutral, creatinine-based glomerular filtration rate (GFR) estimation equations. The performance of these equations in diverse potential living kidney donors requires study.Cross-sectional study.637 from one tertiary hospital with serum creatinine concentration measurement GFR by iohexol plasma clearance between October 2016 December 2020.Creatinine-based Chronic Kidney Disease Epidemiology Collaboration (2009, CKDEPI09; 2021, CKDEPI21)...
Differences in genetic background between control mice and with targeted gene mutations have been recognized as a potential cause for phenotypic differences. In this study, we used A1AR-deficient C57Bl/6 SWR/J congenic to assess the influence of on effect A1AR-deficiency cardiovascular renal functional parameters.In A1AR+/+ A1AR-/- backgrounds, assessed blood pressure heart rate using radio-telemetry, plasma renin concentrations tubuloglomerular feedback.We did not detect significant...
Abstract Background Angiotensin II type‐1 receptor antibody (AT1RAb) has been reported to cause mediated rejection (AMR) in kidney transplant recipients possibly by contraction of renal arteries. We here report 2 with elevated AT1RAbs and negative HLA donor specific antibodies (DSA) anti‐major histocompatibility complex class I chain‐related gene A (MICA) Abs who received therapeutic plasma exchange (TPE) treatment followed IVIG. Case 1 Thirty‐eight‐year‐old patient second for end stage...
To assess the feasibility of using renin promoter for expressing Cre recombinase in juxtaglomerular (JG) cells only, we generated five independent transgenic mouse lines (designated hRen-Cre) under control a 12.2-kb human promoter. In kidneys adult mice mRNA (RT-PCR) was found renal cortex, with protein (immunohistochemistry) being localized afferent arterioles and to lower degree interlobular arteries. levels were regulated renin-typical fashion by changes oral salt intake, water...
You have accessJournal of UrologyTransplantation & Vascular Surgery: Renal Transplantation Surgery II1 Apr 2015MP85-19 TREATMENT OF TRANSPLANT URETER STRICTURES WITH URETERAL DILATION AND URETERONEOCYSTOSTOMY: OUTCOMES FROM A 14 YEAR EXPERIENCE John Stoffel, Randall Sung, Gary Faerber, James Shields, Stuart Wolf, and Yihung Huang StoffelJohn Stoffel More articles by this author , SungRandall Sung FaerberGary Faerber ShieldsJames Shields WolfStuart Wolf HuangYihung View All Author...
Introduction: The individual contribution of Class I and II DSA to AMR is well known, but the DQ-DSA unknown. However an association chronic has been reported in several cohort studies. Current single antigen assays allow detection antibodies against DQ with a specificity sensitivity not possible previous techniques. In this study, we identified group kidney transplant patients only anti-DQ studied relationship between antibody titer, IgG subclasses, ability these fix C1q presence on biopsy....
Gul, Hadia Lala; Sockolov, Macey; Kaur, Amanpreet; Occhipinti, Michelle; Bang, Heejung; Alnimri, Muna; Huang, Yihung; Chen, Ling-Xin; Howes, Katherine A. Author Information