A5066945861- Cholinesterase and Neurodegenerative Diseases
- Computational Drug Discovery Methods
- Pesticide Exposure and Toxicity
- Lipid Membrane Structure and Behavior
- Nicotinic Acetylcholine Receptors Study
- Adenosine and Purinergic Signaling
- Enzyme function and inhibition
- Neurobiology and Insect Physiology Research
- Pancreatic function and diabetes
- Phosphodiesterase function and regulation
- Alzheimer's disease research and treatments
- Surfactants and Colloidal Systems
- Medicinal Plants and Neuroprotection
- Protein Interaction Studies and Fluorescence Analysis
- Chemical synthesis and alkaloids
- Electrochemical sensors and biosensors
- Neonatal Health and Biochemistry
- Electrochemical Analysis and Applications
- thermodynamics and calorimetric analyses
- Enzyme Catalysis and Immobilization
- Chemical Reaction Mechanisms
- Ion channel regulation and function
- Mitochondrial Function and Pathology
- Endoplasmic Reticulum Stress and Disease
- Analytical chemistry methods development
Universidad de Murcia
2006-2023
Instituto Murciano de Investigación Biosanitaria
2016
Abstract Serrated adenocarcinoma (SAC) is a recently recognized colorectal cancer (CRC) subtype accounting for 7.5 to 8.7% of CRCs. It has been shown that SAC poorer prognosis and different molecular immunohistochemical features compared with conventional carcinoma (CC) but, date, only one previous study analyzed its mRNA expression profile by microarray. Using microarray platform, we have studied the signature 11 SACs it 15 matched CC aim discerning functions which characterize biology...
Abstract Human brain acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE) were sequentially extracted, first with a Tris‐saline buffer (S 1 ) then 1% (w/v) Triton X‐100 2 ). About 20 30% of the AChE BuChE activities recovered in S most remaining enzymes . Main molecular forms about 10.5 12.0 S, G 4 BuChE, smaller amounts 4.5 5.5 forms, species measured Application X‐114 phase partitioning affinity chromatography on phenyl‐agarose to revealed that 25% none molecules displayed...
Despite the aberrant expression of cholinesterases in tumours, question their possible contribution to tumorigenesis remains unsolved. The identification kidney a cholinergic system has paved way functional studies, but details on renal are still lacking. To fill gap and determine whether abnormally expressed paired pieces normal cell carcinomas (RCCs) were compared for cholinesterase activity mRNA levels. In studies with papillary RCC (pRCC), conventional RCC, chromophobe oncocytoma,...
Besides esterase activity, acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE) hydrolyze o ‐nitroacetanilides through aryl acylamidase activity. We have reported that BuChE tetramers monomers of human blood plasma differ in ‐nitroacetanilide (ONA) hydrolysis. The homology quaternary structure folding subunits the prevalent species ( ) AChE forms fetal bovine serum prompted us to study amidase activities AChE. k cat / K m values for acetylthiocholine (ATCh), ONA its trifluoro...
The early-onset, irreversible, severe deficits of learning and memory in the senescence-accelerated mouse (SAM)-prone/8 (SAMP8) support its use as an animal model for human dementias early onset. Possible implication acetylcholinesterase (AChE) butyrylcholinesterase (BuChE) cognitive dysfunction SAMP8 mice was studied by comparing cholinesterase (ChE) expression brains their normal control, SAM-resistant/1 (SAMR1) mice. level ChE mRNAs same SAMR1 brains, which agreed with equal AChE activity...
Abstract Half of congenital muscular dystrophy cases arise from laminin α2 (merosin) deficiency, and merosin‐deficient mice ( Lama2dy ) exhibit a dystrophic phenotype. The abnormal development thymus in mice, the occurrence acetylcholinesterase (AChE) gland impaired distribution AChE molecules skeletal muscle mouse mutant prompted us to compare levels mRNAs enzyme species control mice. activity normal (mean ± SD 1.42 0.28 µmol acetylthiocholine/h/mg protein, U/mg) was decreased by ∼50% (0.77...
Apart from its esterase activity, butyrylcholinesterase (BuChE) displays aryl acylamidase (AAA) activity able to hydrolyze o-nitroacetanilide (ONA) and trifluoro-derivative (F-ONA). We report here that, despite amidase sites residing in the same protein, human samples depleted of acetylcholinesterase ratio varied depending on source BuChE. The much faster degradation ONA F-ONA by BuChE monomers (G1) colon kidney than tetramers (G4) suggests aggregation-driven differences AAA site between...