Abstract Background Recent studies have shown that circular RNA (circRNA) is rich in microRNA (miRNA) binding sites. We previously demonstrated the antidepressant effect of ketamine related to abnormal expression various miRNAs brain. This study determined profile circRNAs hippocampus rats treated with ketamine. Methods The aberrantly expressed rat after injection were analyzed by microarray chip, and we further validated these quantitative reverse-transcription PCR (qRT-PCR). target genes...

As one of the model organisms Parkinson's disease (PD) research, zebrafish has its advantages, such as 87% homology with human genome and transparent embryos which make it possible to observe development dopaminergic neurons in real time. However, there is no midbrain system when compared mammals, location projection are seldom reported. In this study, Vmat2:GFP transgenic was used distribution We found that diencephalons (DC) 2 DC4 neuronal populations were detected at 24 h post...

Circular RNA (circRNA) has been recently identified as a critical regulator during carcinogenesis. However, the biological function and potential underlying mechanisms of circRNAs in lung cancer remain to be further elucidated.Here, we first evaluated differentially expressed between tumor matched adjacent nontumor tissues (3 pairs) patients via circRNA microarray. The expression top five dysregulated were tested cell lines circSCAP with concordant alteration microarray data was selected for...

Schistosoma japonicum (S. japonicum) is one of the etiological agents schistosomiasis, a widespread zoonotic parasitic disease. However, mechanism balanced co-existence between host immune system and S. as well their complex interaction remains unclear. In this study, 16S rRNA gene sequencing combined with metagenomic approach, UPLC-MS metabolic profiling was applied to demonstrate changes in gut microbiome community structure during schistosomiasis progression, functional interactions...

Schistosomiasis is a zoonotic and debilitating parasitic disease caused by Schistosoma japonicum. Praziquantel remains the choice for treating schistosomiasis, but its efficacy could be hampered emergence of resistance. In this study, using large-scale drug screening, we selected out myricetin, natural flavonol compound, having good anti-schistosome effect. We found that myricetin exhibited dose time-dependent insecticidal effect on S. japonicum in vitro, with an IC50 600 μM 24 h, inhibited...

Premature ovarian insufficiency (POI) has become one of the greatest health threats to reproduction women during their fertile age. Lycium barbarum polysaccharides (LBPs) are known for anti-aging and reproductive protective functions. Here, we investigated effect LBP on POI mice revealed its possible mechanism by a combination 16S rRNA sequencing metabolomics analysis. In current study, female C57BL/6J treated with D-galactose were used as model investigate reversal degenerative function....

Background Cancer-associated fibroblasts (CAFs) represent the predominant stromal component within tumour microenvironment (TME), exhibiting considerable heterogeneity and plasticity that significantly impact immune response metabolic reprogramming TME, thereby influencing progression. Consequently, investigating CAFs is of utmost importance. The objective this study to employ bibliometric analysis in order evaluate current state research on predict future areas emerging trends. Methods...

Objective: This study aims to assess the effectiveness of coupled plasma filtration and adsorption (CPFA) in patients with SAP through its effect on inflammatory mediators sublingual circulating blood volume. The hypothesis put test is that CPFA can achieve a satisfactory reduction enhance microcirculation very good clinical outcome. Methods: A cohort 112 admitted ICU our institution between January 2018 December 2022 was consecutively recruited. Participants were randomized or control group...

Triatoma rubrofasciata (T. rubrofasciata), one kind of triatomine insects, is the vector Trypanosoma cruzi cruzi), which lead to American trypanosomiasis. Although gut microbiome may play an essential role in development and susceptibility triatomine, there limited research on microbiota T. rubrofasciata. To elucidate effect vector's developmental stages environmental conditions microbiome, we employed 16S rRNA gene sequencing profile bacterial community diversity composition Significant...

Abstract Acute kidney injury (AKI) is a serious disorder associated with significant morbidity and mortality. AKI ischemia/reperfusion (hypoxia/reoxygenation, H/R) can be induced due to several reasons. Paeoniflorin (PF) traditional herbal medicine derived from Paeonia lactiflora Pall. It exerts diverse therapeutic effects, including anti-inflammatory, antioxidative, antiapoptotic, immunomodulatory properties; thus, it considered valuable for treating diseases. However, the effects of PF on...

Small cell lung cancer (SCLC) is a special kind of cancers, lymph or blood metastasis SCLC usually occurs in early stage. Studies breast and colon showed over expression SATB1 could promote tumor growth inhibit apoptosis. Therefore, we studied the SCLC.The level was analyzed tissues, metastatic lymphoid nodes adjacent normal tissues by immunohistochemistry. Meanwhile, small interfering SATB1-targeting RNA constructed transfected into human line NCI-H446 to evaluate effects SATB1-siRNA on...

Hepatocellular carcinoma (HCC) has a poor prognosis due to the rapid disease progression and early metastasis. The metabolism program determines proliferation metastasis of HCC; however, metabolic approach treat HCC remains uncovered. Here, by analyzing liver cell single-cell sequencing data from patients healthy individuals, we found that 6-phosphogluconolactonase (PGLS), cytosolic enzyme in oxidative phase pentose phosphate pathway (PPP), expressing cells are associated with...

Renal interstitial fibrosis (RIF) is the leading cause of end-stage renal disease, partly because lack effective treatments. Curcumin, primary active ingredient in turmeric, reportedly exerts potent antifibrotic effects. This study investigated effects curcumin on RIF unilateral ureteral obstruction (UUO) rats and characterized underlying action mechanism. UUO were treated with for 7 14 d. was evaluated through haematoxylin-eosin staining, Masson type I III collagen expression. Autophagy...

Inflammatory bowel disease (IBD)-related inflammation is closely associated with the initiation and progression of colorectal cancer. IBD generally treated 5-aminosalicylic acid immune-modulating medication, but side effects limitations these therapies are emerging. Thus, development novel preventative or therapeutic approaches imperative. Here, we constructed a dextran sodium sulphate (DSS)-induced mouse model that was infected monosexual Schistosoma japonicum cercariae (mSjci) at day 1...

Probucol is a cholesterol-lowering drug with an anti-proliferative effect. Excessive growth of glomerular mesangial cells and overexpression transforming factor-β1 (TGF-β1) connective tissue factor (CTGF) are the pathological features diabetic nephropathy. In this study, human (HMCs) treated high glucose showed above-mentioned through activation Janus kinase 2 (JAK2)/signal transducers activators transcription (STAT) pathway. can suppress cell proliferation, down-regulate mRNA protein levels...

Breast cancer is the most common type of among females and adenosine triphosphate (ATP) binding cassette E1 (ABCE1) gene a member ATP‑binding (ABC) family. Studies in lung have shown that overexpression ABCE1 tumor cells promotes growth inhibits apoptosis. However, little known about whether associated with breast cancer. In present study, expression was assessed tissue adjacent normal using immunohistochemistry. Furthermore, small interfering (si)RNA targeting constructed transfected into...

In our previous experiment, we found that circRNA-089763 had abnormal levels in the plasma exosomes of patients with postoperative cognitive dysfunction (POCD) after cardiac surgery. Therefore, aim this study was to further investigate relationship between level and POCD elderly non-cardiac A prospective cohort conducted select undergoing elective total 72 were enrolled study, functions assessed one day before three days surgery by a series neuropsychological measurements. Next, divided into...

Wenzi Jiedu Recipe (WJR), traditional Chinese medicine (TCM) formula, has been proven to be clinically useful in the treatment of colorectal cancer (CRC). However, its underlying mechanisms are still elusive, which limits wider application. Thus, we aimed evaluate effect WJR on CRC and elucidate action.Network pharmacology was employed clarify "herb-active ingredient-target" network WJR. The 16S rDNA sequencing method used analyze changes gut microbes mediated by tumor-bearing mice with CRC....

Xiaoai Jiedu recipe (XJR), a classical prescription of traditional Chinese medicine (TCM), has been clinically proven to be effective in ameliorating colorectal cancer (CRC). However, its exact mechanism action is still elusive, limiting clinical application and promotion certain extent. This study aims evaluate the effect XJR on CRC further illustrate underlying action. We investigated anti-tumor efficacy vitro vivo experiments. An integrated 16S rRNA gene sequencing UPLC-MS based...

Our previous study showed that the plasma microRNA-221-3p level could serve as a biomarker for major depression or mood. This aimed to further investigate role of in postoperative cognitive dysfunction (POCD). Patients undergoing non-cardiac surgery were randomly assigned according inclusion and exclusion criteria. POCD was diagnosed by Z score method. The relative decided quantitative real-time polymerase chain reaction. Multiple logistic regression analysis receiver operating...

The present study aimed to explore the clinical characteristics of special adenine-thymine-rich sequence-binding protein 1 (SATB1) in lung adenocarcinoma and its role proliferation, invasion, migration apoptosis cell line A549. expression SATB1 was first studied tumor tissues adjacent non-tumor tissues. siRNA green fluorescent vector constructed transfected into A549, then a fluorescence microscope used transfection efficiency. Western blot analysis adopted measure silencing...