A5006395727- Photosynthetic Processes and Mechanisms
- Spectroscopy and Quantum Chemical Studies
- Photoreceptor and optogenetics research
- Algal biology and biofuel production
- Mosquito-borne diseases and control
- Viral Infections and Vectors
- X-ray Diffraction in Crystallography
- Microbial Community Ecology and Physiology
- Crystallization and Solubility Studies
- ATP Synthase and ATPases Research
- Nitric Oxide and Endothelin Effects
- Genomics and Phylogenetic Studies
- Blood Pressure and Hypertension Studies
- Plant-Microbe Interactions and Immunity
- Antioxidant Activity and Oxidative Stress
- Force Microscopy Techniques and Applications
- Renin-Angiotensin System Studies
- Molecular Junctions and Nanostructures
- Surface Chemistry and Catalysis
- Hormonal Regulation and Hypertension
- Enzyme Structure and Function
- High-Voltage Power Transmission Systems
- Advanced Electron Microscopy Techniques and Applications
- Cardiac Ischemia and Reperfusion
- Insect Resistance and Genetics
Huazhong Agricultural University
2024
West China Hospital of Sichuan University
2013-2024
Southwest University
2019-2024
The First People's Hospital of Guiyang
2024
Sichuan University
2013-2024
Nantong University
2024
Zhejiang Sci-Tech University
2020-2023
Thermo Fisher Scientific (Netherlands)
2020-2023
Qilu Hospital of Shandong University
2019-2023
Wuhan University of Science and Technology
2022-2023
Significance While the current COVID-19 pandemic continues, US Food and Drug Administration (FDA) has approved only one drug against virus—remdesivir. It is a nucleotide analogue inhibitor of SARS-CoV-2 RNA-dependent RNA polymerase; favipiravir another member same class. These nucleoside analogs were originally developed other viral polymerases, can be quickly repurposed should they prove efficacious. We used cryoEM to visualize how favipiravir-RTP binds replicating polymerase determine it...
Endothelin A (ETA) receptor blockade has prevented vascular remodeling in aldosterone and salt-induced hypertension. To evaluate effects of the ETA antagonist, BMS 182874, compared with spironolactone, on aldosterone-infused rats not exposed to a high salt diet, Sprague-Dawley were infused subcutaneously (0.75 microg/h) treated 182874 (40 mg. kg-1. d-1), or hydralazine (both 25 d-1) while receiving normal diet for 6 weeks. Aldosterone increased systolic BP (P<0.01), plasma endothelin...
Reaction center-light harvesting 1 (RC-LH1) complexes are the fundamental units of bacterial photosynthesis, which use solar energy to power reduction quinone quinol prior formation proton gradient that drives ATP synthesis. The dimeric RC-LH1-PufX complex Rhodobacter sphaeroides is composed 64 polypeptides and 128 cofactors, including 56 LH1 bacteriochlorophyll a (BChl a) molecules surround donate two RCs. 3D structure was determined 8 Å by X-ray crystallography, model built with...
Carotenoids protect the photosynthetic apparatus against harmful radicals arising from presence of both light and oxygen. They also act as accessory pigments for harvesting solar energy, are required stable assembly many light-harvesting complexes. In phototrophic bacterium Rhodobacter (Rba.) sphaeroides phytoene desaturase (CrtI) catalyses three sequential desaturations colourless carotenoid phytoene, extending number conjugated carbon–carbon double bonds, N, to nine producing yellow...
Lymphocyte activation gene 3 (LAG-3) is expressed on activated T cells, natural killer cells or B and functions to negatively regulate homeostasis of these cells. Anti-LAG-3 antibodies might be useful for antitumor immunotherapy. In this study, we characterized a novel anti-LAG-3 antibody, LBL-007, which was isolated from human antibody phage display library. LBL-007 found specifically bind LAG-3 antigen, but not CD4 mouse LAG-3. bound promoted interleukin-2 secretion. internalization...
Light-harvesting 2 (LH2) antenna complexes augment the collection of solar energy in many phototrophic bacteria. Despite its frequent role as a model for such complexes, there has been no three-dimensional (3D) structure available LH2 from purple phototroph Rhodobacter sphaeroides. We used cryo-electron microscopy (cryo-EM) to determine 2.1 Å resolution this antenna, which is cylindrical assembly nine αβ heterodimer subunits, each binds three bacteriochlorophyll (BChl) molecules and one...
High-resolution structures of reaction-center light-harvesting 1 complexes provide new insights into quinone dynamics.
Chlorophototrophic organisms have a charge-separating reaction centre (RC) complex that receives energy from dedicated light-harvesting (LH) antenna. In the purple phototrophic bacteria, these two functions are embodied by 'core' photosynthetic component, RC-LH1 complex. complexes sit within membrane bilayer, with central RC wholly or partly surrounded curved array of LH1 subunits bind series bacteriochlorophyll (BChl) and carotenoid pigments. Decades research shown absorption light...
The bacterium Rhodospirillum rubrum contains a simple photosynthetic system, in which the reaction center (RC) receives energy from light-harvesting (LH1) complex. We have used high-resolution atomic force microscopy (AFM) to image two-dimensional crystals of RC-LH1 complex R. rubrum. AFM topographs show that is ∼94 Å height, RC-H subunit protrudes cytoplasmic face membrane by 40 Å, and it sits 21 above highest point surrounding LH1 ring. In contrast, RC on periplasmic side at lower level...
A low dose (0.5 mg/kg) of lipopolysaccharide (LPS), administered 72 hours before 60-minute middle cerebral artery occlusion, induced a delayed neuroprotection proven by the significant decrease (–35%) brain infarct volume in comparison with control, whereas volumes remained unchanged rats treated 12, 24, or 168 ischemia. This neuroprotective effect LPS was only doses (0.25 to 1 mg/kg), this disappeared higher (2 mg/kg). The cortical part infarcted zone, not subcortical part. beneficial on...
Reaction centre light-harvesting 1 (RC–LH1) complexes are the essential components of bacterial photosynthesis. The membrane-intrinsic LH1 complex absorbs light and energy migrates to an enclosed RC where a succession electron proton transfers conserves as quinol, which is exported cytochrome bc1 complex. In some RC–LH1 variants quinols can diffuse through small pores in fully circular, 16-subunit ring, while others missing subunits create gap for quinol export. We used cryogenic microscopy...
The structure of a heptameric LH2 antenna complex reveals energy transfer pathways and basic assembly principles.
This investigation was motivated by a desire to get deeper insight into the mechanism of carotenoiod-to-bacteriochlorophyll (Car-to-BChl) energy transfer proceeding via carotenoid S1 state. (Here, we call 2Ag- and 1Bu+ states "the S2 states" according notation presently accepted.) To systematically examine effect conjugation length on rate efficiency Car(S1)-to-BChl(Qy) transfer, performed following experiments. (1) Subpicosecond time-resolved absorption spectroscopy employed measure...
Treatment of essential hypertensive patients with an AT1 angiotensin receptor antagonist has previously resulted in correction resistance artery structure and endothelial function, whereas a parallel group treated the beta-blocker atenolol there was no improvement altered vascular function. To test hypothesis that could present function if they were subjected to blockade renin-angiotensin system, we crossed over had been randomized treatment irbesartan, studied small before after...
We hypothesized that resistance arteries from diabetic patients with controlled hypertension have less remodeling than vessels untreated hypertensive subjects. Eight normotensive subjects (aged 44±3 years, 3 men; values are mean±SEM), 19 (46±2 9 men), and 23 type 2 diabetes mellitus under antihypertensive treatment (58±1 15 men) were studied. Resistance dissected gluteal subcutaneous tissue assessed on a pressurized myograph. Most (70%) being treated angiotensin-converting enzyme inhibitors....
A three-dimensional model of the dimeric reaction center-light harvesting I-PufX (RC-LH1-PufX) complex from Rhodobacter sphaeroides, calculated electron microscope single particle analysis negatively stained complexes, shows that two halves dimer molecule incline toward each other on periplasmic side, creating a remarkable V-shaped structure. The distribution negative stain is consistent with loose packing LH1 ring near 14th α/β pair, which could facilitate migration quinone and quinol...
Lowering elevated blood pressure (BP) in diabetic hypertensive individuals decreases cardiovascular events. We questioned whether remodeling of resistance arteries from patients would improve after 1 year tight BP control with addition either the angiotensin receptor blocker (ARB) valsartan or beta-blocker (BB) atenolol to previous therapy, which included angiotensin-converting enzyme inhibitors (ACEIs) and/or calcium channel blockers. Twenty-eight type 2 treated oral hypoglycemic and...