A5049154428- Acute Myocardial Infarction Research
- Cardiovascular Effects of Exercise
- Cardiomyopathy and Myosin Studies
- Viral Infections and Immunology Research
- Cell Adhesion Molecules Research
- Muscle Physiology and Disorders
- Signaling Pathways in Disease
- Monoclonal and Polyclonal Antibodies Research
- Cardiac electrophysiology and arrhythmias
- Adipose Tissue and Metabolism
- Molecular Biology Techniques and Applications
- Protease and Inhibitor Mechanisms
- Peptidase Inhibition and Analysis
- Blood properties and coagulation
- Integrated Circuits and Semiconductor Failure Analysis
- Cardiovascular Function and Risk Factors
- Cardiac Imaging and Diagnostics
- Biochemical and Structural Characterization
- Cardiac Ischemia and Reperfusion
- Advanced Proteomics Techniques and Applications
- Electrostatic Discharge in Electronics
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Pleural and Pulmonary Diseases
- Alzheimer's disease research and treatments
- Advanced Biosensing Techniques and Applications
Lomonosov Moscow State University
2013-2024
HyTest (Finland)
2016-2024
Abstract BACKGROUND Cardiac troponin T (cTnT) is an acknowledged biomarker of acute myocardial infarction (AMI) that known to be prone proteolytic degradation in serum. Such usually explained by the action μ-calpain, although there could other candidates for role. In current study, we explored hypothesis thrombin-mediated cTnT cleavage occurs as a result serum sample preparation. METHODS was studied using immunoblotting and mass spectrometry (MS) analysis. RESULTS The comparison isolated...
In the blood of patients with acute myocardial infarction (AMI), cardiac troponin I (cTnI) presents as an intact molecule a repertoire proteolytic fragments. The degradation cTnI might negatively influence its precise immunodetection. this study we identified fragments and calculated their ratio in at different times after AMI to discriminate most stable part(s) cTnI.Serial serum samples were collected from within 1 36 h onset chest pain both before stenting. immunoextracted analyzed by...
The measurement of cardiac isoforms troponin I (cTnI) and T (cTnT) is widely used for the diagnosis acute myocardial infarction (AMI). However, there are conflicting data regarding what forms cTnI cTnT present in blood AMI patients. We investigated as components complexes patients.Gel filtration techniques, sandwich fluoroimmunoassays, Western blotting were used.Plasma samples from patients with contained following complexes: (a) a cTnI-cTnT-TnC complex (ITC) composed full-size 37 kDa or its...
Abstract Background Current studies suggest that cardiac troponin (cTn) forms in the circulation may vary different clinical scenarios. Our aim was to design a combination of cTn assays specific main and evaluate their analytical performance. Methods We developed immunoassays for measuring (1) long-cTnT cTnI-cTnT-TnC (ITC) ternary complex, with cTnT long form without cleavage at C-terminal amino acids residue 189–223, designated “long-cTnT ITC complex assay;” (2) both plus short-cTnT...
Abstract Background Increased cardiac troponin (cTn) concentrations occur in acute myocardial injury and chronic diseases. Characterization of cTn composition the circulation may assist differentiating etiologies injury. Our goal was to study kinetics patients following type 1 infraction (T1MI), procedures, heart diseases establish relationship between clinical diagnosis. Methods Plasma samples were collected from 201 with T1MI, 78 undergoing surgeries, 218 cardiomyopathy or failure. Major...
Autoantibodies to cardiac troponins (TnAAbs) could negatively affect troponin I (cTnI) measurements by TnAAbs-sensitive immunoassays. We investigated the epitope specificity of TnAAbs and its influence on cTnI immunodetection in patients with acute myocardial infarction (AMI).The was studied immunoassays gel-filtration experiments. The endogenous 35 plasma samples from 15 AMI.The inhibitory effect observed only for ternary complex (I-T-C) not binary (I-C) or free cTnI. In same...
Blood measurement of cardiac troponin T (cTnT) is one the most widespread methods acute myocardial infarction (MI) diagnosis. cTnT degradation may have a significant influence on precision immunodetection; however, there are no consistent data describing level and sites proteolysis in blood MI patients. In this study, we bordered major fragments quantified their relative abundance at different times after MI.Serial heparin plasma samples were collected from 37 patients 2-37 h following onset...
Interactions of key amyloidogenic proteins with SARS-CoV-2 may be one the causes expanding and delayed post-COVID-19 neurodegenerative processes. Furthermore, such abnormal effects can caused by their fragments circulating in body during vaccination. The aim our work was to analyze effect receptor-binding domain coronavirus S-protein (RBD) on alpha-synuclein amyloid aggregation. Molecular modeling showed that predicted RBD complex monomeric is stable over 100 ns molecular dynamics. Analysis...
Heparin is a highly charged polysaccharide used as an anticoagulant to prevent blood coagulation in patients with presumed myocardial infarction and prepare heparin plasma samples for laboratory tests. There are conflicting data regarding the effects of on measurement cardiac isoforms troponin I (cTnI) T (cTnT), which immunodiagnosis acute infarction. In this study, we investigated influence immunodetection human troponins.
Immunodetection of cardiac isoforms troponin I (cTnI) and T (cTnT) in blood samples is widely used for the diagnosis acute myocardial infarction. The complex (ITC-complex), comprising cTnI, cTnT, C (TnC), makes up a large portion troponins released into bloodstream after necrosis cardiomyocytes. However, stability ITC-complex has not been fully investigated. This study aimed to investigate samples. A native was incubated buffer solutions, serum, citrate, heparin, or EDTA plasma at various...
About half of the mutations that lead to hypertrophic cardiomyopathy (HCM) occur in MYBPC3 gene. However, molecular mechanisms pathogenicity point cardiac myosin-binding protein C (cMyBP-C) remain poorly understood. In this study, we examined effects D75N and P161S substitutions C0 C1 domains cMyBP-C on structural functional properties C0-C1-m-C2 fragment (C0-C2). Differential scanning calorimetry revealed these disorder tertiary structure C0-C2 molecule. Functionally, mutation reduced...
Pediatric dilated cardiomyopathy (DCM) is a rare heart muscle disorder leading to the enlargement of all chambers and systolic dysfunction. We identified novel de novo variant, c.88A>G (p.Lys30Glu, K30E), in
Tropomyosin (Tpm) mutations cause inherited cardiac diseases such as hypertrophic and dilated cardiomyopathies. We applied various approaches to investigate the role of troponin (Tn) especially T (TnT) in pathogenic effects Tpm cardiomyopathy-associated M8R, K15N, A277V, M281T, I284V located overlap junction neighboring dimers. Using co-sedimentation assay viscosity measurements, we showed that TnT1 (fragment TnT) stabilizes WT all mutants studied except M8R. However, isothermal titration...
We characterized a novel genetic variant c.292G > A (p.E98K) in the TPM1 gene encoding cardiac tropomyosin 1.1 isoform (Tpm1.1), found proband with phenotype of complex cardiomyopathy conduction dysfunction and slow progressive neuromuscular involvement. To understand molecular mechanism by which this mutation impairs function, we produced recombinant Tpm1.1 carrying an E98K substitution studied how affects structure molecule its functional properties. The results showed that N-terminal...
Adiponectin (Adn) is a protein that circulates in the blood several oligomeric forms, namely low-, medium-, and high-molecular-weight forms. Adn may serve as risk factor for type 2 diabetes mellitus (T2DM). The aims of this work were (1) to produce monoclonal antibodies (MAbs) specific different (2) design immunoassays suitable measuring forms present human blood, (3) investigate changes occur patients with T2DM. Gel filtration, fluoroimmunoassays, Western blotting utilized major techniques...