A5044401948- Microbial Natural Products and Biosynthesis
- Genomics and Phylogenetic Studies
- Geomagnetism and Paleomagnetism Studies
- Metalloenzymes and iron-sulfur proteins
- Magnetic and Electromagnetic Effects
- Plant Disease Resistance and Genetics
- Geology and Paleoclimatology Research
- Ammonia Synthesis and Nitrogen Reduction
- Wastewater Treatment and Nitrogen Removal
- Microbial Metabolic Engineering and Bioproduction
- Microbial Fuel Cells and Bioremediation
- Fungal and yeast genetics research
- Biofuel production and bioconversion
- Bacterial Genetics and Biotechnology
- Enzyme Catalysis and Immobilization
- Photosynthetic Processes and Mechanisms
- Legume Nitrogen Fixing Symbiosis
- Microbial Community Ecology and Physiology
- Esophageal Cancer Research and Treatment
- Electrocatalysts for Energy Conversion
- Algal biology and biofuel production
- Plant-Microbe Interactions and Immunity
- Geochemistry and Elemental Analysis
- Protist diversity and phylogeny
- Mycobacterium research and diagnosis
First Affiliated Hospital of Zhengzhou University
2019-2024
Guiyang Medical University
2024
Zhengzhou University
2008-2023
China Agricultural University
2013-2022
Nanjing University of Aeronautics and Astronautics
2020
Beijing Institute of Geology for Mineral Resources
2009-2017
University of Minnesota
2007-2015
Peking University People's Hospital
2013-2015
Peking University
2013-2015
Xiamen University
2014
Most biological nitrogen fixation is catalyzed by molybdenum-dependent nitrogenase, an enzyme complex comprising two component proteins that contains three different metalloclusters. Diazotrophs contain a common core of nif genes encode the structural subunits and components required to synthesize However, complement enable diazotrophic growth varies significantly amongst fixing bacteria archaea. In this study, we identified minimal gene cluster consisting nine in genome Paenibacillus sp....
We provide here a comparative genome analysis of 31 strains within the genus Paenibacillus including 11 new genomic sequences N2-fixing strains. The heterogeneity genomes (15 and 16 non-N2-fixing strains) was reflected in large size shell genome, which makes up approximately 65.2% genes pan genome. Large numbers transposable elements might be related to heterogeneity. discovered that minimal compact nif cluster comprising nine nifB, nifH, nifD, nifK, nifE, nifN, nifX, hesA nifV encoding...
Abstract Metabolic reprogramming has been observed in cancer metastasis, whereas metabolic changes required for malignant cells during lymph node metastasis of esophageal squamous cell carcinoma (ESCC) are still poorly understood. Here, we performed single-cell RNA sequencing (scRNA-seq) paired ESCC tumor tissues and nodes to uncover the microenvironment (TME) pathways. By integrating analyses scRNA-seq data with metabolomics plasma samples, found nicotinate nicotinamide metabolism pathway...
An improved strategy was developed for the high-density culture of Magnetospirillum gryphiswaldense strain MSR-1 and large-scale magnetosome production in both 7.5- 42-liter autofermentors. By using a nutrient-balanced feeding replacement carbon nitrogen sources to reduce accumulation Na(+) Cl(-) ions, we reduced factors that tend inhibit cell growth, particularly increase osmotic potential. Semicontinuous thereby achieved autofermentor first time. When cells were harvested at 36 73 h,...
Enzymatic biodiesel production by transesterification in solvent media has been investigated intensively, but glycerol, as a by-product, could block the immobilized enzyme and excess n-hexane, solution aid, would reduce productivity of enzyme. Esterification, solvent-free no-glycerol-release system for production, developed, two-step catalysis soybean oil, hydrolysis followed esterification, with Yarrowia lipolytica lipase is reported this paper.First, oil was hydrolyzed at 40°C 100 U broth...
ABSTRACT Avermectins produced by Streptomyces avermitilis are commercially important anthelmintic agents. The detailed regulatory mechanisms of avermectin biosynthesis remain unclear. Here, we identified SAV3619, a TetR-family transcriptional regulator designated AveT, to be an activator for both production and morphological differentiation in S. . AveT was shown indirectly stimulate affecting transcription the cluster-situated gene aveR directly repressed its own ( aveT ), adjacent pepD2...
BldD generally functions as a repressor controlling morphological development of Streptomyces. In this work, evidences that also activates antibiotic production are provided. Streptomyces roseosporus (which produces daptomycin widely used for treatment human infections), deletion bldD notably reduced production, but enhanced sporulation. stimulated by directly activating transcription dpt structural genes and dptR3 encodes an indirect activator production), repressed its own gene....
ABSTRACT Magnetotactic bacteria (MTB) synthesize unique organelles termed “magnetosomes,” which are membrane-enclosed structures containing crystals of magnetite or greigite. Magnetosomes form a chain around MamK cytoskeletal filaments and provide the basis for ability MTB to navigate along geomagnetic field lines in order find optimal microaerobic habitats. Genomes species genus Magnetospirillum , addition gene encoding tubulin-like FtsZ protein (involved cell division), contain second “...
Magnetotactic bacteria (MTB) synthesize unique organelles, the magnetosomes, which are intracellular nanometer-sized, membrane-enveloped magnetite. The biomineralization of magnetosomes involves uptake large amounts iron. However, iron metabolism MTB is not well understood. genome magnetotactic bacterium Magnetospirillum gryphiswaldense strain MSR-1 contains two ferrous transport genes, feoB1 and feoB2. FeoB1 protein was reported to be responsible mainly for play an accessory role in...
ABSTRACT Daptomycin produced by Streptomyces roseosporus is an important lipopeptide antibiotic used to treat human infections caused Gram-positive pathogenic bacteria, including drug-resistant strains. The genetic basis for regulatory mechanisms of daptomycin production poorly known. Here, we characterized the dptR3 gene, which encodes a MarR family transcriptional regulator located adjacent known biosynthetic ( dpt ) genes. Deletion reduced significantly and delayed aerial mycelium...
Avermectins produced by Streptomyces avermitilis are effective anthelmintic agents. The autoregulatory signalling molecule that triggers avermectin biosynthesis is a novel butenolide-type molecule, avenolide, rather than common γ-butyrolactones (GBLs). We identified AvaR2, pseudo GBL receptor homologue, as an important repressor of and avenolide cell growth. AvaR2 directly repressed transcription aveR (the ave cluster-situated activator gene), aco (a key gene for biosynthesis), its own...
A moderate-temperature, astaxanthin-overproducing mutant strain (termed MK19) of Phaffia rhodozyma was generated in our laboratory. The intracellular astaxanthin content MK19 17-fold higher than that wild-type. TLC profile showed a band for an unknown carotenoid pigment between those β-carotene and astaxanthin. In the present study, we attempted to identify enhance synthesis by overexpression crtS gene encodes synthase (CrtS).A crtS-overexpressing constructed without antibiotic marker....
Ammonia is a major signal that regulates nitrogen fixation in most diazotrophs. Regulation of by ammonia the Gram-negative diazotrophs well-characterized. In these bacteria, this regulation occurs mainly at level nif (nitrogen fixation) gene transcription, which requires nif-specific activator, NifA. Although Gram-positive and diazotrophic Paenibacilli have been extensively used as bacterial fertilizer agriculture, how regulated response to availability bacteria remains unclear. An...
ABSTRACT The role of a tetR transcriptional regulatory gene ( SAV7471 ) in avermectin production the Gram-positive soil bacterium Streptomyces avermitilis was investigated by deletion, complementation, and overexpression experiments. Gene deletion open reading frame resulted overproduction. also SAV7472-SAV7473 transcripts, which encode protein unknown function flavoprotein possibly involved pantothenate coenzyme A (CoA) metabolism. EMSAs footprinting assays showed that can bind to two...
Most biological nitrogen fixation is catalyzed by molybdenum-dependent nitrogenase, an enzyme complex comprising two component proteins that contains three different metalloclusters.Diazotrophs contain a common core of nif genes encode the structural subunits and components required to synthesize metalloclusters.However, complement enable diazotrophic growth varies significantly amongst fixing bacteria archaea.In this study, we identified minimal gene cluster consisting nine in genome...