A5018265535- Genomics and Chromatin Dynamics
- Developmental Biology and Gene Regulation
- Epigenetics and DNA Methylation
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Physiological and biochemical adaptations
- Genetics, Aging, and Longevity in Model Organisms
- vaccines and immunoinformatics approaches
- Pluripotent Stem Cells Research
- RNA modifications and cancer
- Neurobiology and Insect Physiology Research
- SARS-CoV-2 and COVID-19 Research
- RNA Research and Splicing
- Reproductive Biology and Fertility
- Aquaculture Nutrition and Growth
- Advanced biosensing and bioanalysis techniques
- SARS-CoV-2 detection and testing
- Biosensors and Analytical Detection
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- RNA Interference and Gene Delivery
- Ubiquitin and proteasome pathways
- Evolution and Genetic Dynamics
- Prenatal Screening and Diagnostics
- Cardiac Fibrosis and Remodeling
- Bacillus and Francisella bacterial research
- Hippo pathway signaling and YAP/TAZ
Babraham Institute
2023-2025
Indian Institute of Science Education and Research Pune
2020-2024
Abstract Flying insects have invaded all the aerial space on Earth and this astonishing radiation could not been possible without a remarkable morphological diversification of their flight appendages. Here, we show that characteristic spatial expression profiles levels Hox genes Antennapedia ( Antp ) Ultrabithorax Ubx underlie formation two different organs in fruit fly Drosophila melanogaster . We further demonstrate appendage morphology is dependent specific doses. Interestingly, find wing...
Food is fundamental for the survival of organisms, governing growth, maintenance, and reproduction through provision essential macronutrients. However, access to food with optimum macronutrient composition, which will maximize evolutionary fitness an organism, not always guaranteed. This leads dietary mismatches potential impacts on organismal performance. To understand consequences such mismatches, we examined effects isocaloric diets varying in composition eight key traits spanning across...
Abstract Background During the latter stages of their development, mammalian oocytes under dramatic chromatin reconfiguration, transitioning from a non-surrounded nucleolus (NSN) to surrounded (SN) stage, and concomitant transcriptional silencing. Although NSN-SN transition is known be essential for developmental competence oocyte, less about accompanying molecular changes. Here we examine changes in transcriptome DNA methylation during NSN SN mouse oocytes. Results To study dynamics...
Nlrp5 encodes a core component of the subcortical maternal complex (SCMC) cytoplasmic protein structure unique to mammalian oocyte and cleavage-stage embryo. NLRP5 mutations have been identified in patients presenting with early embryo arrest, recurrent molar pregnancies imprinting disorders. Correct patterning DNA methylation over imprinted domains during oogenesis is necessary for faithful genes. It was previously shown that oocytes mutation human SCMC gene KHDC3L had globally impaired...
During mammalian embryo development, pluripotent epiblast cells diversify into the three primary germ layers, which will later give rise to all fetal and adult tissues. These processes involve profound transcriptional epigenetic changes that require precise coordination. Peptidylarginine deiminase IV (PADI4) is a regulator strongly associated with inflammation carcinogenesis but whose physiological roles are less well understood. We previously found Padi4 expression pluripotency. Here, we...
Developmental processes have to be robust but also flexible enough respond genetic and environmental variations. Different mechanisms been described explain the apparent antagonistic nature of developmental robustness plasticity. Here, we present a “self-sufficient” molecular model development particular flight organ that is under control Hox gene Ultrabithorax ( Ubx ) in fruit fly Drosophila melanogaster . Our based on candidate RNAi screen additional analyses all converge an autonomous...
<title>Abstract</title> <bold>Background:</bold> During the latter stages of their development, mammalian oocytes under dramatic chromatin reconfiguration, transitioning from a non-surrounded nucleolus (NSN) to surrounded (SN) stage, and concomitant transcriptional silencing. Although NSN-SN transition is known be essential for developmental competence oocyte, less about accompanying molecular changes. Here we examine changes in transcriptome DNA methylation during NSN SN mouse...
Abstract Food is fundamental for the survival of organisms, governing growth, maintenance, and reproduction through provision essential macronutrients. However, access to food with optimum macronutrient composition, which will maximize evolutionary fitness an organism, not always guaranteed. This leads dietary mismatches potential impacts on organismal performance. To understand consequences such mismatches, we examined effects isocaloric diets varying in composition eight key traits...
Abstract Background Comparative analysis of Chromatin immunoprecipitation followed by massively parallel sequencing (ChIP-seq), combined with transcriptomics data (RNA-seq), can provide critical insights into gene regulatory networks controlled transcription factors in a given biological context. While multiple programs exist, which need to be individually installed and executed generate such information, user-friendly tool low system requirements combines the various computational processes...
Abstract Hox genes encode Homeodomain-containing transcription factors, which specify segmental identities along the anterior-posterior axis. Functional changes in have been directly implicated evolution of body plans across metazoan lineage. The protein Ultrabithorax (Ubx) is expressed and required developing third thoracic (T3) segments holometabolous insects studied so far, particularly, order Coleoptera, Lepidoptera Diptera. Ubx function key to differential development second (T2) T3...
Abstract The wastewater-based epidemiology (WBE) of SARS-CoV-2 is a quick and cost-effective method tracking virus transmission. However, few studies reported on campus or in academic residential settings worldwide. In this study, we demonstrated the WBE approach to detect, monitor, evaluate genomic variants fragments sewage treatment plant (STP) located CSIR National Chemical Laboratory, Pune, India. Herein describe early warning capability WBE, with viral load rise water up 14 days before...
Modern response to pandemics, critical for effective public health measures, is shaped by the availability and integration of diverse epidemiological outbreak data. Tracking variants concern (VOC) integral understanding evolution SARS-CoV-2 in space time, both at local level global context. This potentially generates actionable information when integrated with
Abstract Background Comparative analysis of Chromatin immunoprecipitation followed by massively parallel sequencing (ChIP-seq), combined with transcriptomics data (RNA-seq), can provide critical insights into gene regulatory networks controlled transcription factors in a given biological context. While multiple programs exist, which need to be individually installed and executed generate such information, user-friendly tool low system requirements combines the various computational processes...
Summary The modern response to pandemics, critical for effective public health measures, is shaped by the availability and integration of diverse epidemiological outbreak data. Genomic surveillance has come forefront during coronavirus disease 2019 (COVID-19) pandemic at both local global scales identify variants concern. Tracking concern (VOC) integral understanding evolution severe acute respiratory syndrome 2 (SARS-CoV-2) in space time. Combining phylogenetics with data like case...