A5031379331- HIV Research and Treatment
- Mosquito-borne diseases and control
- HIV/AIDS Research and Interventions
- HIV/AIDS drug development and treatment
- Hematopoietic Stem Cell Transplantation
- Sexuality, Behavior, and Technology
- HIV-related health complications and treatments
- Intestinal and Peritoneal Adhesions
- T-cell and Retrovirus Studies
- Bacterial Identification and Susceptibility Testing
- Vector-Borne Animal Diseases
- RNA Interference and Gene Delivery
- Antimicrobial Resistance in Staphylococcus
- Uterine Myomas and Treatments
- Adenosine and Purinergic Signaling
- Endometriosis Research and Treatment
- Virus-based gene therapy research
- Animal Disease Management and Epidemiology
- Gender, Feminism, and Media
- Clostridium difficile and Clostridium perfringens research
- Immune Cell Function and Interaction
University of Michigan
2020-2024
Lakeland Health
2023
Corewell Health
2023
The University of Texas Health Science Center at Houston
1990
Assess the efficacy of staged interventions aimed to reduce inappropriate Clostridioides difficile testing and hospital-onset C. infection (HO-CDI) rates. Interrupted time series. Community-based. National Healthcare Safety Network (NHSN) metrics from January 2019 November 2022 were analyzed after three at a community-based healthcare system. Interventions included: (1) an electronic medical record (EMR) based hard stop requiring confirming ≥3 loose or liquid stools over 24 h, (2) infectious...
HIV-1 Vpr is necessary for maximal HIV infection and spread in macrophages. Evolutionary conservation of suggests an important yet poorly understood role macrophages pathogenesis. counteracts a previously unknown macrophage-specific restriction factor that targets reduces the expression Env. Here, we report macrophage mannose receptor (MR), targeting Env primary human monocyte-derived acts synergistically with Nef to target distinct stages MR biosynthetic pathway dramatically reduce...
Nef is an HIV-encoded accessory protein that enhances pathogenicity by down-regulating major histocompatibility class I (MHC-I) expression to evade killing cytotoxic T lymphocytes (CTLs). A potent inhibitor restores MHC-I needed promote immune-mediated clearance of HIV-infected cells. We discovered the plecomacrolide family natural products restored surface Nef-expressing primary cells with variable potency. Concanamycin (CMA) counteracted at subnanomolar concentrations did not interfere...
The human immunodeficiency virus (HIV)-encoded accessory protein Nef enhances pathogenicity by reducing major histocompatibility complex I (MHC-I) cell surface expression, protecting HIV-infected cells from immune recognition. Nef-dependent downmodulation of MHC-I can be reversed subnanomolar concentrations concanamycin A (1), a well-known inhibitor vacuolar ATPase, at below those that interfere with lysosomal acidification or degradation. We conducted structure–activity relationship study...
To inhibit endocytic entry of some viruses, cells promote acidification endosomes by expressing the short isoform human nuclear receptor 7 (NCOA7) which increases activity vacuolar ATPase (V-ATPase). While we found that HIV-1 infection primary T led to endosomes, NCOA7 levels were only marginally affected. Contrastingly, 50 kDa form sodium/hydrogen exchanger 6 (NHE6) greatly reduced. NHE6 overexpression and low concentrations V-ATPase inhibitor, concanamycin A, selectively reversed endosomal...
Abstract Background Clostridioides difficile infections are a frequent cause of nosocomial and large financial burden to the health care system. Molecular diagnostics such as PCR may frequently detect colonized patients lead overdiagnosis. Using electronic medical record (EMR) system, we implemented multiple interventions improve testing stewardship. Methods Over span 3 years, our Infection Control (IC) team separate interventions: in July 2019, hard stop was implemented, requiring providers...