A5020202632- Neuroscience and Neuropharmacology Research
- Hearing, Cochlea, Tinnitus, Genetics
- Neural dynamics and brain function
- Biochemical Analysis and Sensing Techniques
- Photoreceptor and optogenetics research
- Photochromic and Fluorescence Chemistry
- Hearing Loss and Rehabilitation
- Animal Behavior and Reproduction
- Amphibian and Reptile Biology
- Connexins and lens biology
- Neuroscience and Neural Engineering
- Nicotinic Acetylcholine Receptors Study
- bioluminescence and chemiluminescence research
- Radical Photochemical Reactions
- Trace Elements in Health
- Neuroscience and Music Perception
- Neurobiology and Insect Physiology Research
- Ion channel regulation and function
- Pediatric health and respiratory diseases
- Retinal Development and Disorders
- Bat Biology and Ecology Studies
- Plant and animal studies
- Antimicrobial Peptides and Activities
- Acoustic Wave Phenomena Research
- Marine animal studies overview
University of Pittsburgh
2012-2023
Center for the Neural Basis of Cognition
2005-2022
Carnegie Mellon University
2011-2022
Institute of Neurobiology
2020
Swanson Center
2017
Eye and Ear Foundation
2008-2016
Philipps University of Marburg
2005-2006
University of Kansas
2000
Howard Hughes Medical Institute
1998
Duke University Hospital
1995-1998
Abstract Although the connections of auditory brainstem nuclei are well described in adult mammals, almost nothing is known concerning how and when these develop. The purpose present study was to describe development efferent projections cochlear nucleus (CN), first central relay station ascending pathway mammals. We used two tracers rats aged between embryonic day 15 (E15) postnatal 14 (P14; birth rat at E22 = P0). carbocyanine dye DiI applied into CN aldehyde‐fixed tissue. second tracer,...
In contrast to our knowledge about the anatomical development of mammalian central auditory system, its physiological properties is still poorly understood. order better understand developing brainstem, we made intracellular recordings in brainstem slices from perinatal rats examine synaptic transmission superior olivary complex, first binaural station ascending pathway. We concentrated on neurons lateral olive (LSO), which adults, are excited ipsilateral side and inhibited contralateral...
During brain development, endogenously generated coordinated neuronal activity regulates the precision of developing synaptic circuits (Shatz and Stryker, 1988; Weliky Katz, 1997). In neonatal neocortex, a form endogenous is present as locally restricted intercellular calcium waves that are mediated by gap junctions (Yuste et al., 1992). As in other non-neuronal systems, these fluctuations may basis functional cell assemblies (for review, seeWarner, 1992; Peinado 1993b). study, we...
Abstract During brain development, GABA and glycine switch from being depolarizing to hyperpolarizing neurotransmitters. This conversion results a gradual decrease in the chloride electrochemical equilibrium potential ( E Cl ) of developing neurons, which correlates an increase expression or activity potassium cotransporter, KCC2. However, evidence as whether KCC2 is sufficient, itself, induce this lacking. In order address question, we used gain‐of‐function approach by over‐expressing human...
Hearing loss leads to a host of cellular and synaptic changes in auditory brain areas that are thought give rise perception deficits such as temporal processing impairments, hyperacusis, tinnitus. However, little is known about possible circuit connectivity may underlie these hearing deficits. Here, we show mild result brief noise exposure pronounced reorganization local excitatory inhibitory circuits the mouse inferior colliculus. The exact nature reorganizations correlated with presence or...
Sound processing in the cochlea is modulated by cholinergic efferent axons arising from medial olivocochlear neurons brainstem. These contact outer hair cells mature and inner during development activate nicotinic acetylcholine receptors composed of α9 α10 subunits. The subunit necessary for mediating effects on as genetic deletion results functional de-efferentation cochlea. Cholinergic modulation spontaneous cochlear activity before hearing onset important maturation central auditory...
Cochlear outer hair cells (OHCs) are known to uniquely participate in auditory processing through their electromotility, and like inner cells, also capable of releasing vesicular glutamate onto spiral ganglion (SG) neurons: this case, the sparse Type II SG neurons. However, unlike signaling at cell-Type I neuron synapse, which is robust across a wide spectrum sound intensities, OHC-Type synapse weaker has been hypothesized occur only intense, possibly damaging levels. Here, we tested ability...
Sub-lethal activation of cell death processes initiate pro-survival signaling cascades. As intracellular Zn(2+) liberation mediates neuronal pathways, we tested whether a sub-lethal increase in free could also trigger neuroprotection. Neuronal transiently increased following preconditioning, and was both necessary sufficient for conferring excitotoxic tolerance. Lethal exposure to NMDA led delayed that contributed significantly excitotoxicity non-preconditioned neurons, but not tolerant...
The inferior colliculus (IC) in the mammalian midbrain is major subcortical auditory integration center receiving ascending inputs from almost all brainstem nuclei as well descending thalamus and cortex. In addition to these extrinsic inputs, IC also contains a dense network of local, intracollicular connections, which are thought provide gain control contribute selectivity for complex acoustic features. However, contrast organization afferents, development functional intrinsic connections...
Introducing 3-methoxy or 3,5-dimethoxy substituents on the 4-hydroxyphenacyl (pHP) photoremovable protecting group has been explored with two excitatory γ-amino acids, l-glutamic acid and butyric (GABA). These significantly extend absorption range of pHP chromophore, e.g., tail bands 2a,b above 400 nm, well beyond absorptions aromatic amino acids nucleotides. Irradiation releases rate constants ∼107 s-1 appearance efficiencies (Φapp) 0.03−0.04. The photoproducts are formed through excited...
Abstract Although hearing onset occurs relatively late during ontogeny of rats [around postnatal day (P) 12], anatomical brainstem connections are formed much earlier and present before birth, indicating that a substantial amount maturation without acoustic input. Electrical activity is thought to influence neuronal development, but the physiological properties auditory neurons perinatal barely known. The study focuses on development electrophysiological membrane in rat's superior olivary...