Enzymes have replaced or decreased usage of toxic chemicals for industrial and medical applications leading toward sustainable chemistry. In this study, we report purification characterization a biofilm degrading protease secreted by Microbacterium sp. SKS10. The was identified as metalloprotease, Peptidase M16 using mass spectrometry. It showed optimum activity at 60°C, pH 12 retained its in the presence various salts organic solvents. enzyme able to degrade biofilms efficiently...

Proteases are one of the largest groups hydrolytic enzymes constituting about 60% total worldwide sales industrial due to their wide applications in detergent, leather, textile, food and pharmaceutical industry. Microbial proteases have been preferred over animal plant because fundamental features ease production. Bacillus infantis SKS1, an alkaline protease producing bacteria has isolated from garden soil north India identified using morphological, biochemical molecular methods. 16S rDNA...

Background: Chronic liver disease (CLD) is a major global health issue, with growing number of cases and significant impact on patient survival. The plays crucial role in lipid metabolism, including synthesizing regulating lipoproteins. study was done to determine the development more accurate predictive models for CLD progression, complementing existing tools such as Model End-Stage Liver Disease (MELD) Child-Pugh scores. Materials methods: A prospective observational cohort conducted at...

Background: In India, cirrhosis and chronic liver disease (CLD) were responsible for 2.1% of all fatalities. One the most significant clinical prognostic consequences is acute kidney Injury (AKI). this context, renal progression AKI a warning indication risk death. It has been noted that vascular constriction in patients increases resistive index (RRI). The study aims to evaluate predictive significance duplex Doppler ultrasonography group who are not azotemic. Materials Methods: was...

Abstract Lethality of Plasmodium falciparum caused malaria results from ‘cytoadherence’, which is mainly effected by exported erythrocyte membrane protein 1 (PfEMP1) family. Several P . proteins (exportome) including chaperones alongside cholesterol rich microdomains are crucial for PfEMP1 translocation to infected surface. An Hsp40 (heat shock 40) ‘PFA0660w’ functions as a co-chaperone ‘PfHsp70-x’, and these co-localize specialized intracellular mobile structures termed J-dots. Our studies...

We report the synthesis and crystal structure of a naphthalimide–methoxyquinoline (NI–HQ) based molecular rotor for visualizing sweat pores without degradation dsDNA present in sweat.

Superoxide dismutase (SOD) plays a central role in cellular defence against oxidative stress and is of pharmaceutical importance. The SOD from Potentilla atrosanguinea (Pa-SOD) unique enzyme as it possesses free-radical scavenging capability at temperatures ranging between 263 353 K. crystal structure recombinant Pa-SOD has been determined to 2.3 A resolution. active-site residues are well ordered additional water molecules present place bound copper ion. There significant difference the...

Plasmodium falciparum encodes a novel repertoire of the helical interspersed subtelomeric (PHIST) family exported proteins, which play diverse roles in infected red blood cells, contributing to malaria pathogenesis. PHIST proteins are central parasite biology and modify human erythrocytes by interacting with host proteins. Here, we have attempted understand localization function two unexplored PHISTc subfamily, PFD1140w PF11_0503, compared these well-characterized member, PFI1780w. We...

Superoxide dismutase (SOD) from Potentilla atrosanguinea (Wall. ex. Lehm.) was crystallized using 20% PEG 3350 and 0.2 M ammonium iodide diffraction data were collected to 2.36 Å resolution an in-house Cu Kα X-­ray source. Analyses show that with a redundancy of 3.2 sufficient determine the structure by SAD technique iodine anomalous signal. This is lower than in previous cases which protein structures determined iodines for phasing copper X-ray sources. Cocrystallization proteins halide...

The malarial parasite Plasmodium falciparum has two nucleosome assembly proteins, PfNapS and PfNapL (Chandra, B. R., Olivieri, A., Silvestrini, F., Alano, P., Sharma, A. (2005) Mol. Biochem. Parasitol. 142, 237-247). We show that both interact with histone oligomers but only is able to deposit histones onto DNA. This property of divalent cation-dependent ATP-independent. Deletion the terminal subdomains abolishes its capabilities, truncated protein retains ability bind histones. Both binding...

Abstract Background Ribosome biogenesis is an energy consuming and stringently controlled process that involves hundreds of trans-acting factors. Small nucleolar RNAs (snoRNAs), important components ribosome are non-coding guide involved in rRNA processing, nucleotide modifications like 2'-O-ribose methylation, pseudouridylation possibly gene regulation. snoRNAs ubiquitous diverse their genomic organization, mechanism transcription maturation. In vertebrates, most present introns protein...

Alkaline proteases from microbial sources have been found suitable for diverse industrial applications, with serine being the most common enzymes used in detergent industry. In present study, we purified and characterized an extracellular alkaline protease Microbacterium paraoxydans sp. SKS10. The was using ammonium sulfate precipitation followed by different chromatography techniques (fold purification 6.919). Km Vmax were determined to be 0.183 mg/mL 4.904 U/mL, respectively. This enzyme...

Plasmodium falciparum (Pf) proteins exported to infected erythrocytes are key effectors of malaria pathogenesis. These include the PfEMP1 (Pf erythrocyte membrane protein 1) family that affects malaria-related mortality through cytoadhesion and parasite immune evasion. Parasites also induce membranous structures called Maurer's clefts (MC) in compensate lack host synthetic export machinery. is mediated by a myriad including Pf skeleton binding 1 (PfSBP1) PF70, hypothetical 16 member. Here,...

Introduction:: MKT-077 and its derivatives are rhodacyanine inhibitors that hold potential in the treatment of cancer, neurodegenerative diseases malaria. These allosteric drugs act by inhibiting ATPase action heat shock proteins 70 kDa (HSP70). accumulates mitochondria displays differential activity against HSP70 homologs. Methods:: The four Plasmodium falciparum HSP70s (PfHSP70) present various subcellular locations to perform distinct functions. In study, we have used bioinformatics tools...

The apicomplexan pathogenic parasite

Background: Plasmodium falciparum (P. falciparum) heat shock proteins (PfHSP70s) are an important class of molecules critically involved in parasite survival during stress. Interaction between the cytosolic PfHSP70-1 and a crucial lipid modulator, Phosphatidylinositol 3 Phosphate (PI3P), stabilizes Digestive Vacuole (DV) to facilitate hemoglobin trafficking breakdown, turn impacting survival. Methods: PI3P binding on is facilitated by its C-terminal LID domain that controls substrate...